LOCUS       Exported               14070 bp ds-DNA     linear   SYN 14-JUN-2021
DEFINITION  Expresses channelrhodopsin fused to mCherry for monosynaptic circuit
            mapping and optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    N2c(dG)-hChR2-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14070)
  TITLE     Jessell Rabies Virus CVS-N2c DG
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14070
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             5499..5510
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(7302..7313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10510..10528)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10524..10571
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(10599..10615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10599..10615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10612..10634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10623..10639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10647..10677)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10692..10713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10830..10847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11001..11589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11081..11100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11760..12620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12383..12402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12621..12725)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12752..13207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12839..12858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13049..13070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13333..13355
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13347..13364
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13348..13364
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        13434..13737
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13738..13941
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13892..13912
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     13986..14005
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13986..14004
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcgtgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccgctagc ggcgcctagc cggtcatcct tttgacgatt
     3361 ccagtcccga ggataacctc ctctcgggat tggggggaat ctttggatcc agtagtcctc
     3421 cttgaactcc gtccaacagg gcagattcaa gagtcataag actttcatta atcatttcag
     3481 ttgatcagac atggtcgtgt agattctctt aatacgggag atcttctagc agtttcagtg
     3541 accaacggtg ctttcattct ccaggaactg ataccaaagg ttgtggacag gccaaggggt
     3601 acttcggatg actctgtgct tgggcacaga aagaggtcgt agtgtgcccc ccgatagcgg
     3661 actcgacatg aatcaactaa gaaaggcaat ctgcctccca tgatggacat aagcaatagt
     3721 tcacaaccat cttgcatctc agtgaagtgt acataactat tgagggctgg gtcatctaag
     3781 catttcagtc gagaaaaaaa ctgtagacca aaagaacaac tagcaacact tctcatccag
     3841 agacccatat caagatgcta gatccgggag aggtttatga tgaccctatt gatccaattg
     3901 agtcagaggc tgaacccaga ggaaccccca ctgtccccaa catcttgagg aactccgact
     3961 acaatctcaa ttctcctttg atagaggatc ctgccaaact aatgttagaa tggttgaaga
     4021 cagggaacag accttatcgg atgactttga cagacaattg ctccaggtct tacaaagttt
     4081 tgaaagatta tttcaagaaa gtagatttgg gttctctcaa agtgggcgga actgctgcac
     4141 agtcaatggt ttctctctgg ttgtgtggtg cccactctga atcaaacagg agccggagat
     4201 gtataaccga cttggcccat ttctattcca agtcatcccc catagagaag ctattgaatt
     4261 gtacgctagg aaacagaggc ctgagaatcc caccagaggg ggtgttaaat tgcctcgaga
     4321 gggtcaatta tgacaaggca tttgggaggt atctggccaa cacgtattcc tcttacttgt
     4381 ttttccatgt aatcacctta tacatgaatg ccttagactg ggaagaggaa aagaccatcc
     4441 tggcattatg gaaagatata acctcagtgg ataccgagaa ggacttggtc aaattcaaag
     4501 atcaaatatg gggactgttg attgtgacaa aggactttgt ttactctcag agttcaaact
     4561 gtctttttga cagaaactac acactgatgc taaaggatct tttcttgtct cgattcaact
     4621 ccttaatgat tctgctttct ccccctgagc cccgatactc agatgactta atatctcagc
     4681 tgtgccagct atacatcgct ggggatcaag tcttgtccat gtgtgggaac tccggctatg
     4741 aagtcatcaa aatattggag ccatatgtcg tgaacagttt ggtccagagg gcagagaagt
     4801 ttaggcctct catccacccc ttgggagact ttcctatgtt cataaaagac aaggtgaatc
     4861 aacttgaagg gacttttggt cccagtgcaa agaggttttt tagggtttta gatcaattcg
     4921 acaacataca tgacttagta tttgtgtatg gctgttacag acattggggg cacccctata
     4981 tagattatcg gaagggtctg tcgaaactat atgatcaagt tcacattaag aaagtaatag
     5041 ataagtccta ccaggagtgt ttagcaagtg acttggccag aaggatcctc agatggggat
     5101 ttgacaagta ctccaaatgg tatctagatt cgagattcct tgcccgagac caccccttga
     5161 ctccttatgt caagacccaa acatggccac ccaaacatat agtagacttg gtgggggaca
     5221 catggcataa gctcccgatc acgcagatct ttgaaattcc tgaatcaatg gacccgtcag
     5281 agatactgga tgacaaatca cattctttca ccagaacaag gttagcttct tggctgtcag
     5341 agaaccgagg ggggcctgtt cctagcgaga aggtcattat cacggccctg tctaagccac
     5401 ctgtcaatcc ccgagagttt ttgaaatcta tagacctcgg aggattgcca gatgaggatt
     5461 tgataattgg cctcaaacca aaggaacggg agttgaagat cgagggccga ttctttgctc
     5521 taatgtcatg gaatctaaga ttatattttg tcatcaccga aaagctcttg gccaactaca
     5581 ttttgccact ttttgacgca ctgactatga cagacaacct gaacaaggtg ttcaaaaagt
     5641 tgatcgacag ggtcaccggg caagggcttt tggactattc tagggtcaca tacgcatttc
     5701 acctggacta tgagaaatgg aacaatcatc aaagattgga gtcaacagag gatgtattct
     5761 ctgtcctaga tcaggtgttt ggattgaaga gggtgttttc tagaacacac gagttttttc
     5821 agaagtcctg gatctattat tcagacagat cagacctcat tgggttatgg gaggaccaaa
     5881 tatattgctt ggatatgtct aacggcccaa cctgctggaa tggccaagat ggcgggctag
     5941 agggcttgcg gcagaagggc tggagtctag tcagtttatt aatgatagat agagaatctc
     6001 aaaccaggaa cacaagaacc aagatactag ctcaaggaga caaccaggtt ctgtgtccta
     6061 catacatgtt gtcaccggga ttgtctcaag aggggcttct ctatgagtta gagagcatat
     6121 caaggaatgc actctcaata taccgagcta tcgaggaagg agcatctaag ctggggctga
     6181 tcatcaagaa ggaagagacc atgtgtagtt atgactttct catatatggg aagaccccct
     6241 tatttcgagg caacatattg gtgcctgaat ccaaaagatg ggcccgagtc tcttgcatct
     6301 ctaacgacca aatagtcaac ctcgccaata taatgtcgac agtatccacc aatgcgctga
     6361 cagtggcaca acactctcaa tctctgatca aacctatgag ggattttctg ctcatgtcag
     6421 tacaggcagt tttccactac ctgttgttta gcccaatcct aaaaggcaga gtttataaga
     6481 ttctgagtgc tgaaggggag agctttctcc tagccatgtc gcggataatc tacctagatc
     6541 cttctttggg aggggtgtct ggaatgtctc tcgggaggtt ccatatacgt cagttctcag
     6601 accctgtctc tgaagggttg tcattctgga gagagatctg gttaggctct catgagtcct
     6661 ggattcacgc gttgtgtcag gaggccggga accccgatct tggagagaga acactcgaga
     6721 gcttcactcg ccttttagaa gatcctacta ccttaaatat caaaggaggg gccagcccta
     6781 ccattctact caaggatgct atcagaaagg ctctgtacga cgaggtggac aaggtggaaa
     6841 attcagagtt tcgagaggca atcctgctgt ccaagaccca tagagataac tttatactct
     6901 ttttaaaatc tgttgagcct ctgttccctc gatttctcag tgagctcttc agttcgtctt
     6961 tcttgggaat accggagtca atcattggac tgatacaaaa ctcccggaca ataagaaggc
     7021 agtttagaaa gagtctctca agaactttag aagagtcctt ctacaactca gagatccacg
     7081 ggattaatcg gataacccag acacctcaaa gggtcggaag ggtgtggcct tgctcttcag
     7141 agagggcgga tctacttagg gagatctctt gggggaggaa agtggtaggc acgacagttc
     7201 ctcacccttc cgagatgttg gagttgtttc ccaaatcctc catttcctgc acttgtggag
     7261 caacaggggg aggcaatcct agagtctctg tatcagtact cccgtccttc gatcagtcat
     7321 ttttctcacg gggcccccta aagggatact tgggctcgtc cacctctatg tcaacccagc
     7381 tattccatgc atgggaaaaa gtcactaatg ttcatgtggt gaaaagggct ctatcgttaa
     7441 aagaatctat aaactggttc atcaatagga attccaattt ggctcaaact ctaattggaa
     7501 acatcatgtc tctgacaggc cctgacttcc ctctagaaga ggcccctgtt ttcaaacgga
     7561 cagggtcagc cttgcatagg ttcaagtctg ccagatacag cgaaggaggg tattcttctg
     7621 tttgccctaa ccttctctcc catatctctg ttagtacaga cactatgtct gatttgaccc
     7681 aaaacgggaa gaactatgat ttcatgtttc agccattgat gctttatgcg caaacatgga
     7741 catcggaact ggtacagagg gatacaagac ttagagactc cacgtttcac tggcaccttc
     7801 ggtgcaacag atgtgtgagg cccattgatg atataacact ggaaacttct cagatcttcg
     7861 agttcccgga tgtgtcaaaa aggatatcca ggatggtttc tggagctgtc cctcaatttc
     7921 agaagcttcc tgatatccgt ctaagaccag gtgattttga atccctaagt ggtagagaaa
     7981 agtctcgcca tatagggtca gctcaggggc tcttatactc aatcttagta gcaattcacg
     8041 actcaggata caatgatggg accatcttcc ctgtcaacat atacggcaaa gtttccccca
     8101 gagactattt gagagggctt gcaagaggga tcttgatagg gtcctcgatt tgcttcttga
     8161 cacgaatgac aaatattaac attaacagac ctcttgaatt gatctcaggg gtaatttcct
     8221 atattctcct gaggctggat aatcatccct ctctgtatat aatgctcaga gaaccgtctc
     8281 ttagaggaga aatattctct atccctcaga aaatccccgc cgcttaccca accactatga
     8341 gagaaggcaa cagatcgatt ttgtgttacc tccaacacgt gctacgctat gagcgagagg
     8401 caatcacggc gtccccggag aatgactggc tgtggatctt ctcagacttc agaagtgtga
     8461 aaatgacgta cttgaccctc attacttacc agtctcacct cctactccag agggttgaga
     8521 gaaacttgtc taagagtatg agagctactc tgcgacaaat gggttcctta atgaggcaag
     8581 tgctgggtgg gcacggagaa gataccttgg agtcagacga tgacattcaa cgattactaa
     8641 aagactcttt gcgaaagaca aggtgggtgg atcaagaggt gcgccatgca gctagaacca
     8701 tgaatggaga ttacagcccc gacaagaaag tatcccgtaa ggtaggatgt tcagaatggg
     8761 tctgctctgc tcaacagatt gccgtctcca cctcagccaa cccggcccct gtctcagagc
     8821 ttgacattag ggccctctct aagcggtttc aaaacccctt gatctcgggc ctgagagtgg
     8881 ttcagtgggc aaccggtgcc cattataagc ttaagcctat tctagatgat ctaaatgttt
     8941 tcccatctct ctgtcttgta attggagacg ggtcaggggg aatatcaagg gcagttctca
     9001 acatgtttcc agattctaag cttgtgttca acagcctatt ggaggtgaat gatctgatgg
     9061 cttccggaac acatccactg cctccttcag caatcatgag tggaggagat gacatcatct
     9121 ccagagtgat agactttgac tcaatatggg agaaaccgtc cgacctgagg aactcggcca
     9181 cctggagata cttccagtcg gttcaaaaac aggtcaacat gtcgtatgac ctcattattt
     9241 gtgatgcaga agttactgat attgcatcta tcaaccggat aactctgttg atgtctgatt
     9301 tcgcattgtc tatagatgga ccactttatc tggtcttcaa aacttacggg actatgctag
     9361 taaacccgga ctataaagct attcaacatc tgtcaagagc gttcccttcg gtcacagggt
     9421 ttgtaaccca agtaacttca tccttttctt ctgagctata cctccggttc tctaaacgag
     9481 gaaagttttt cagggacgcc gagtacttga cctcttccac ccttcgagag atgagccttg
     9541 tgttgttcaa ttgcagcagc cccaaaagtg agatgcagag agctcgttcc ttaaactatc
     9601 aagacctggt aaggggattt cctgaagaga tcatatcaaa tccttacaac gaaatgatca
     9661 taactctgat tgacaatgat gtagagtcct tcctagtcca caagatggtg gatgatcttg
     9721 agctacagag gggaactctg tctaaagtgg ctatcattat atccatcatg atcgtttttt
     9781 ccaatagagt cttcaacatt tccaaacctt tgactgaccc cttgttctat ccaccgtctg
     9841 atcctaaaat cctgaggcac ttcaacatat gttgcagtac tatgatgtat ctatctaccg
     9901 ctttaggcga cgtccctaac ttcgcaagac ttcatgacct gtataacaga cctataactt
     9961 gttacttcag aaaacaagtt attcgaggga atatttatct atcttggagt tggtccgatg
    10021 ataccccagt gttcaagaga gtagcctgta attctagctt gagtctgtca tctcactgga
    10081 tcaggttgat ctacaagata gtgaagacta ccagactcat tggcagcata aaagacctat
    10141 caggagaggt agaacgacat cttcatgggt ataacagatg gatcaccctc gaggatatcc
    10201 gatctagatc atccctacta gattacagtt gtttgtaagc cggatattac cgaaagcctg
    10261 tgcatgctaa aattcttgta tgatgcatct tgaaaaaaac aagatcttga atccggacct
    10321 ctggttgttt gattgttttt tccatcttta ttgttttttt gttaagcgtg ggtcggcatg
    10381 gcatctccac ctcctcgcgg tccgacctgg gcatccgaag gaggacgcac gtccactcgg
    10441 atggctaagg gaggggcggg gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
    10501 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
    10561 ggggtttttt gctgaaagtc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
    10621 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10681 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10741 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10801 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10861 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10921 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10981 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11041 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11101 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11161 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11221 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11281 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11341 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11401 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    11461 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11521 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11581 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11641 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11701 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11761 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11821 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11881 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11941 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12001 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12061 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12121 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12181 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12241 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12301 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12361 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12421 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12481 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12541 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12601 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12661 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12721 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
    12781 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    12841 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    12901 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    12961 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    13021 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    13081 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    13141 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    13201 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    13261 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    13321 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcctagttat
    13381 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    13441 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    13501 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    13561 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    13621 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    13681 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
    13741 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
    13801 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
    13861 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
    13921 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
    13981 gaaattaata cgactcacta tagggagacc caagctggct agattaagcg tctgatgagt
    14041 ccgtgaggac gaaacccggc gtaccgggtc
//
