LOCUS       Exported                 993 bp ds-DNA     linear   SYN 10-JUN-2021
DEFINITION  Express flag-tagged mouse Tet1 catalytic domain - catalytic dead 
            mutant..
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3-Flag-Tet1 CD Mut
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Ito S, Shen L, Dai Q, Wu SC, Collins LB, Swenberg JA, He C, Zhang Y
  TITLE     Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 
            5-carboxylcytosine.
  JOURNAL   Science. 2011 Sep 2;333(6047):1300-3. doi: 10.1126/science.1210597. 
            Epub 2011 Jul 21.
  PUBMED    21778364
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          116..688
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             734..757
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tagagtcgat cctgagaact tcagggtgag
      121 tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg
      181 gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc
      241 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
      301 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt
      361 taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca
      421 aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat
      481 taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat
      541 tggtagaaac aactacatcc tggtcatcat cctgcctttc tctttatggt tacaatgata
      601 tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca
      661 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggttt gtgctgtcga
      721 ccccaagctt atggactaca aagacgatga cgacaaggga tccgaagctg caccctgtga
      781 ctgtgatgga ggtacacaaa aagaaaaagg cccatattat acacaccttg gggcaggacc
      841 aagtgtggct gctgtcaggg agctcatgga gactaggttt ggccagaagg ggaaggcaat
      901 ccggattgag aagatagtgt tcacggggaa ggaagggaag agctctcagg gctgcccggt
      961 cgccaagtgg gtgatcagaa gaagtggtcc tga
//
