LOCUS Exported 21053 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter. ACCESSION . VERSION . KEYWORDS pEMS1538 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 21053) AUTHORS Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM TITLE Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. JOURNAL Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. PUBMED 30765420 REFERENCE 2 (bases 1 to 21053) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..21053 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..67 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" intron 2363..2495 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" regulatory 2545..2554 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2563..5610 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" primer_bind complement(2565..2587) /label=M13/pUC Forward /note="In lacZ gene" protein_bind 5614..5660 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." polyA_signal 5783..5864 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5820..5839) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 13509..13530 /label=F1ori-F /note="F1 origin, forward primer" promoter 13693..13797 /gene="bla" /label=AmpR promoter CDS 13798..14658 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(18865..18887) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 18973..19113 /label=bom /note="basis of mobility region from pBR322" primer_bind 19028..19047 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(19128..19145) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(19299..19887) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(19379..19398) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(20058..20918) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(20919..21023) /gene="bla" /label=AmpR promoter ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccac gcgtggcgcg ccggagaaga tcaagactca ggtgggtgga 181 gatgactgcc ccccatgcac acatttcctg gtgctccacc ccctcagcaa atgacttctg 241 ggtaagactg agggataaag cctatactgt tgatccttgc ccagctcaca gctgggatat 301 ttgggtagac ctcactagga ccctctcctt tacaccttag cctcccactg agagcattaa 361 gtgggcgaat tttgatgcct cccagctttc ccagctctta cctcctgccc ctggctattt 421 tctgttctta cctcctgctc cttgttttct gtccatagtg ggtgggggaa gcagaaagag 481 gaatgagaag gagagggatt gggatcttga ccccaacttc aaggccctca ccttctgtgg 541 agccctgagt ttggtccaca gggaacattc caacctctag tcattgctcc caccagattc 601 caggtccaca tcctgaaggc ttcatccctg tcccactagg atcctcccag gccctgggat 661 ccctcttttt ccctccacac tctgcctccc actcttctcc ctggctcctt tgcaccctcc 721 acgagcaaag atgaacactc cagaagttgc agcaaatgag ggtgaacatg ggccctgtag 781 aaccccagaa gtaggagttt ccagccatcc tcaatcaggc atcccaggac caggcactta 841 gcaggagacc tgcccattcc caggcctcct ccacccctgg gctcaagcag tgagagagga 901 ctttgagaat gccagttttg acagtacccg ttccatactg caaggctgca gaaggtgggc 961 ttctgggctg gagcctcatc ttcagccagc atcctcctta gcctgcctct ccagagccac 1021 gaagccccat aggtacccag agagactcca ggccacttgg gaaccacatc aacatctttg 1081 catgagttag aaataggaat ccttttcttg gagtggattc cagccttgta cagtgcacaa 1141 ccctgtctta acccctccac ctctccagcc tcgttcaggg atagccagaa tgggagtagt 1201 ggtgagggtg ggtggcagac tgggaggaca ggggatgggg aaggatggga gcagaatagt 1261 tgtacaggga agtagcaggg ggctcaggaa cccatcaggg aaagccagga cagattctca 1321 acatctttag aggccttcag cagtccaagc cactactaat ttatggggac tgaatgaatg 1381 agaaaaggag gggagggaga aggatgagag ggagagagaa ggctggaggg gagatgagag 1441 tcggagagaa aagcctagga ggtaaagaga gacctagaag ggggcgggga ggacagaaga 1501 gggggccacc catcataagt tgccaggaga gatggcccag caccaggcct gaggcttggg 1561 ggccccagaa aggcaggaaa cgggccaaag caagaaggag acggggaagg ggttcgggtc 1621 tctagggaag agcagagagt gggagaagag gaggagggag cggggatgcg ggcagagata 1681 aagggagcaa cggctgcagg gccgggcgcg cagcgacagg cgagagggag ggaagcggaa 1741 atttaaaagt gaagatgcag ataacgcagc ctggagacgg gaacccgggt ggggatagag 1801 ggagggaggg ggaggagagt gagcggcgag tggagcggga gccgactgga agaagggctc 1861 tagggagggg gctgtggctg ctggggtccg aggtggggcc gggtacacca gccccatcac 1921 tgtttgcaga gagtcaggga ggcggaaaag acacgcgctc taggctccca tcagggcaca 1981 tggcccggac ccatcccccg cgcgtctccc cggctgcggg gcgcgggggg ctgccgggtg 2041 cgcttggctg tggcgcggcg cgttggagac tttattgcga tgggacgata agaggggcgg 2101 gggcggggtc ctgggggccg aggcggcagc gctttaatta aaacggaaat tgcggccccg 2161 ggccgcgcgg gggccggagg gttccaagcg gccccttagc tggaagcgtt tctccaggac 2221 ccccccgcaa cccccgccac gcccgggctg ccccctcccg ccaggccctg ccggacccgg 2281 cgccgtcttc tcctccttgt cacccgcggt cgcttcgggc ggggatcggt gccaccgagc 2341 gcaaagcctg cctcggcgcg ccgtaagtat caaggttaca agacaggttt aaggagacca 2401 atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg 2461 tcttactgac atccactttg cctttctctc cacaggaagt tcctattccg aagttcctat 2521 tcttcaaaag gtataggaac ttccgccacc atggcggatc ccgtcgtttt acaacgtcgt 2581 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 2641 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 2701 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg 2761 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt 2821 tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt 2881 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta 2941 caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc 3001 aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc 3061 gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc 3121 agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg 3181 ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat 3241 ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta 3301 cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc 3361 ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac 3421 gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa 3481 ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag 3541 gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt 3601 aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag 3661 gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac 3721 catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat 3781 attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg 3841 gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc 3901 atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg 3961 atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg 4021 gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct 4081 gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc 4141 ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag 4201 gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg 4261 ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat 4321 acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat 4381 ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa 4441 accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg 4501 atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca 4561 caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc 4621 tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc 4681 agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc 4741 cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag 4801 cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa 4861 caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc 4921 gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc 4981 cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg 5041 ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa 5101 acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc 5161 gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg 5221 gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt 5281 tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa 5341 aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac 5401 ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat 5461 ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt 5521 ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac 5581 cattaccagt tggtctggtg tcaaaaataa taaaagttcc tattccgaag ttcctattct 5641 ctagaaagta taggaacttc agcggccgcg actctagatc ataatcagcc ataccacatt 5701 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa 5761 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 5821 caatagcatc acaaatttca caaataaagc atttttttca ctgcgaattc tcgtaaaact 5881 cttcatggca gtagttatta ttctctctct ctctcttttt cttttttctt gagacaggat 5941 atttctctgt tgcccaggct ggagtgcagt ggcacagtct tggctcactg cagcctggac 6001 ctcctgggct caagccatcc tcccacctca gcctcccaag tagctggggc tacaggcaca 6061 tggccaccag gcccagataa tttttcattt ttgtagagac tgagtctcac catgttaccc 6121 aggtttatta ttctcatttt ttagatgaag agactgaggt ccagagaagc tcaatgactt 6181 gcctagtttt acaaatctcc tgccatcaca tacccctcag cgtccttaat aagagggagg 6241 ccaccaacta tgtgctgggc actgtggtgg atgctggagc tatagggttg agtatataag 6301 aaatggtgtt gctggagcaa ctgttgcttg cttacctgac ctatctgaga attaattagc 6361 aggggaacat atttttgttt tcagattcaa tataagaact tgtgtgggca aaaataaaga 6421 tcagtagtaa taacagtagt tcccatttgc tgactgtact gtcctaagtg catatatata 6481 tacatacaca cacgcatacc tatactcctc taatactcaa aatgatcctg tttatgtatt 6541 gttaatatgc tcattttatt tttaaatttt tatttatttt tatttttatt tatttttgag 6601 acggagtctc attctgtcgc ggaggctgaa gtgcagtggt gcgatctcag ctcagtgcga 6661 cctccgcctc ccgggttcaa gtgattctcc tgcctcagcc tccggactag ctgggattac 6721 aggcgcccgc ctccacgccc agctaatttt tgtattttta gtagagatgg ggtttcgcca 6781 tgttggccag gctggtctcg tactcctgac cttgagtgat ccacctgcct cggcctccca 6841 aagtgctggg attacaggca tgagccaccg cgccgggcta atatgctcat tttagtgagg 6901 caaaaataga ggctcagagt ctgatttgta caaaactaca gagcagttaa gtgtcctctc 6961 agatgtgtac cctgatctgg gtgactctag gactctaggt ctcaactgtt acaaccagtt 7021 aagggtttgg ggaagcactg ggccaagagt caggaaaatg gaagccacag gtagtgcaag 7081 gtcttgggaa tgggacgtct ggtccaagga ttcacgcgat gactggaacc cgaagagccg 7141 gggcccggtt tacggccgcc atgaagcaac gcgcgccggt aggtttggga atcagggagc 7201 cctctgaata ggagactgag ttgggaggga aaggggcttc gctgggggag cctcggcttc 7261 ttctgggaga aaattcccac ggctacctag tgagcctgca aactggtagg cgccggcgta 7321 ggcgcgcggg cggggccggg ggcggggcct gcggggcgtg gcggggcggg cagagggcgg 7381 ggcctgcttc tcctcagctt caggcggctg cgacgagccc tcaggcgaac ctctcggctt 7441 tcccgcgcgg cgccgcctct tgctgcgcct ccgcctcctc ctctgctccg ccaccggctt 7501 cctcctcctg agcagtcagc ccgcgcgccg gccggctccg ttatggcgac ccgcagccct 7561 ggcgtcgtgg tgagcagctc ggcctgccgg ccctggccgg ttcaggccca cgcggcaggt 7621 ggcggccggg ccctgaggcg cgggatcgat ccatgacacc cccccgcccc ccatatctca 7681 ctataattca ctgtcaaatg gcttttatga tttggagttt ggggatactt ttaaagacag 7741 ggtcttggta gctcctagat ggcttgggat tcacttagta acccttgctg gcctaaaatt 7801 caggaacttc tacctcatct tcccaagtac tgggttagta gggatgtcca gcatatttag 7861 cttgctttaa tgattttgct gtactttttt ctgtgtgtac aggtgcatgt atatttataa 7921 aactaacata atatatttta cccccaaagt accataaccc taattaatgc tattaaatgt 7981 tttgtatgct gggctctggg catactaata taaccaatat tatacaatgc aaaacaattt 8041 tggaatatct tctgtaaaat tatcttatta gtaagttgta accatctcag acttctaagt 8101 ttgagtttgt aaaggatcag ttttttttgg tgacctacac acacacacac acacacacac 8161 acacacacac acacaggggg gtggattctg agaatttaga gaatttagat ggttaatacc 8221 agtttttatt tttattctgt gtgatattta tttattaaga tttatttatt tattacatgt 8281 aggtacactg tatctgtctt cagacacacc agaagagggc atcagatctc gttacagatg 8341 gttgtgagtc accatgtggt tgctgggatt tgaactcagg acctctggaa gaacagtcag 8401 tgctcttaac cgttgagcca tctcaccagc cccgtgatat tttatttaac ttactagaat 8461 atactgtgtt aaaacatatt ttaggggatt ggggagatgg cccattctgt aaagtgtctg 8521 ccatgtcatc ttcaggacct gcgtttgtaa ccccagcatg gagaagacag agacagggag 8581 acccctgggc ttcctggcaa gctagactag caaatttggt taactaggtt caatgagaga 8641 ctcagtctca aaacataaga agtgaatttt ttagacccaa tttcaatctc tgaactccat 8701 acgtatgcac atgtacctgc atataaatat atatacacac ccataaacac atgcacacac 8761 aaaaaatggt tcaaaagttc ttaaaatgta taagagtaga aagtgtagag ccgggagtgg 8821 tggcgcacgc ctttaatccc agcacttggg agcagaggca ggtggatttc tgagttcgag 8881 gccagcctgg tctacagagt gagttccagg acagccagag ctatacagag aaatcctgcc 8941 tcaaaaaacc aaaaataaag tgtatatact aatctttcat gcctaaaata tttatttttg 9001 ccacattttt catacctctt taatattttt ctttttctct ttgtgcttca aaggaaattt 9061 tgtgtcatgt tttacttttc attcttgact ctattcagca gtaagacgca gcatggcttt 9121 cttagagaag tataatctat tacaaaacgg taacatttaa ctgctctaca taccctcttg 9181 tagctaaatt cctctgttaa actaagactt attatctaaa cgtaattaaa tgttgatatc 9241 ttataatgct ccactttgaa acagctgtct ttcttttgca gattagcgat gatgaaccag 9301 gttatgacct agatttgttt tgtataccta atcattatgc cgaggatttg gaaaaagtgt 9361 ttattcctca tggactgatt atggacaggt tagtaagacc tcgattgaag ttatttttag 9421 agggtgtgtg tgtggtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtaagaga 9481 gagaggagag gcatgtgtga tatatatgtg aagtgtattg catgtacatg gaatccagag 9541 aaggacatta ggtgtcctac tctgttgctc tcttattccc ttgaatcagt gtctcttgct 9601 gaacctgggg catacatact gatttggcta ggctgcctgg ctagcaagac ccagggatct 9661 gcttgtctct gccccccagt gctgacgtta caagcaggtg caaccacatc tggtttttat 9721 atgggtactg ggggatctga acttagggcc tgatgcgtgc ccaagtgctc tcacccgcca 9781 agccacctct ccagccaaag ggtttttttt cttgtattaa ttctaactta gcaactgttg 9841 agtagttgat gtttgaatat aagctaagcc aacttattag aataaatgtt ttctattttt 9901 tacttgtatg tgtgagtgcc tgcatgtatg tatgtgaagc atatgtattc caggatgtca 9961 gaattgtgat ggattccctg gaactggagt tacagaccat tgtgaaccac tgtgtatggg 10021 tgctgggaac tgaactttag gtcctctgca aaaacagtaa ggatcttaac tgataaacta 10081 tctttctagc cctaaggctt tctactttta aagttcgttt ctctttctct ctctctctct 10141 ctctctctct ctctctctcc ctctccctcc ctcctcctcc tcctcctctt tcccccctct 10201 cgcctcctct ttctctctcc cctccctctc tctctctcac acacacacac agacagagag 10261 aggagagaga aaggaagcct tactaattaa ttacctagtt ttagacataa ctagtgggaa 10321 tccatagtaa gtaaatcaac attcttgaag agaggttaga agaattggct ctcgttgtcc 10381 atcgaggctg tataagatta ttcttctgtt ttctcagctt accatacata ttttctacgt 10441 agacaatacc aattcaaata cactgtacgt aaagtatatg cgcacacaca cacacacaca 10501 cacacacaca cacacacaag ttacatattg tcatatagta ctaaaattta tggacttttt 10561 catattaata catctagagc tatcatgttt tttatctttt aaaaattact ttacggggct 10621 acagagatgt ctcagcagtt aggagcactg actgctcttc cagaggtcct gagttcaatt 10681 cccagcaacc acatggtggc tcacaaccat ctgtaatggg atctgatcca ctcttatggt 10741 gtgtctgaag acagtgacag tgtactcata tacatgaaat aaatctttaa aaaattactt 10801 taccatcagg gtatatatat atatatatat gtgtgtgtgt gtgtgtgtgt gtatgaagta 10861 tttgctattt gagagggtct cactgtttag ctcagtctgg cttggaacgt tctgtatagc 10921 ccagactggc ctcaactcag cagtccccct gaaaggctct catatgtgga gattacaggt 10981 atgtgctact gtgcccagcc ctacatgtta ctttgttttt gaaatagggt atcaaatagc 11041 ttagactggc ttctaagtca gtgtgtagct aaggatggtc tgagcttctg aacctgctac 11101 ttcaactcct ggtgtgccat catggccagt ttgcacagtt gtgagagtag aactcaggac 11161 ctcctatgtg ttactagaca tgttctctac cagctgagct acatcccaat tcgcctacat 11221 ttacttttat tttttaaaag atttatttat gtatacagca ctctgcctgc atgtacatct 11281 gcatgccaga agagggcacc agatctcatc atacatagtt gtgagccacc atgtggttgc 11341 tggggattga actcagggcc tctggaagag cagtcagtgc tcttaacctc tgagccatct 11401 ctgtagcccc ctacatttat tttaaagatt ataggctatt ctattcatag atgtgccata 11461 actgatttaa ccagatcttc attattttgt acttagtaga gtatattaga gggaatgaat 11521 cttaaataca cattttgaca cttgctcagc tttagacaag taaaaatcac tggtcaagga 11581 aatggtgctt agtggatgct tctgaactgt caaattgtcc tccaacaagt tacatcattt 11641 tatacttcca acgttatgtg agagttcatt tttctttagg ctcttctagt attttatatt 11701 tgagaaataa aatttaacca aaatttgcca atctagtaag cttgttttta tttgtatctt 11761 taaattttat gatagatgtt atagtgtact ctcctctccc ctcccctccc ctctcctcct 11821 cagcaggatc ttgttatgta gccctaaggg atctaatact tactctgtag tccaggctgt 11881 ccttcagctc atgagacaca ccatcatgct tggcatgttt cttgttctga aaggtatctg 11941 gttttaattt tggttgagct ggtgtgttgg tgtatatctg taatctcagc acaggcaagt 12001 agagacaagg attatgatta cagtccagcc tgggctattt agagggattc tctttaatct 12061 ttcctcatgc cccaaaatct tacctttggt atatgaaaaa tagtctccac ttctgcaaaa 12121 tattgcttta tgaagtaaga attaattccc ttcatagaga caaggaatgt gtcctgtaaa 12181 agtttaatgt gtaagaagta tttgttataa aagataaata ttcagaatct tctttttaat 12241 tcctgatttt atttctatag gactgaaaga cttgctcgag atgtcatgaa ggagatggga 12301 ggccatcaca ttgtggccct ctgtgtgctc aaggggggct ataagttctt tgctgacctg 12361 ctggattaca ttaaagcact gaatagaaat agtgatagat ccattcctat gactgtagat 12421 tttatcagac tgaagagcta ctgtgtaagt ataattaact tataattaaa aaaatagggc 12481 cattctagtt ttatctatat tttttttaaa cttgtgcaaa ctatgctaca taatcaactt 12541 acttgattat gtaatatata tagtaatagt tggaattact attattacat atttattttt 12601 ctgtgctggg gtagaaccca gggaactaag tactgtaata accgagcttc attcatccta 12661 gtcattttta attttttttt ttttttttgt tttttcgaga caaggtttct ctgtgtagcc 12721 ctggctgtcc tggaactcac tctgtagacc aggctggcct cgaactcaga aatctgcctg 12781 cctctgcctc ccagtgctga gattaatgac gtgcaccacc actgcctggc atttttttaa 12841 agttttaatt tgagaccctt tctcactaac ttgctcacac tatcctcgaa ctcattacgt 12901 gggctggtct agaaatttca gtcaacttgc ctcagcttct caagtagctg ggattacagg 12961 caggtgctac cacttctggc catgatcatt gttgttgttg ctattattat tattattatt 13021 tcactgatac ttcatatcac aacacagaat agtaactgta gttcatgacc aacaaaaaag 13081 ttgtacagtt tctctgttgt cttattttga tctggatttt gatttgtttc atggcactgg 13141 gcaggctaat ctcaaactgc atggctcaaa tagtccttct gtcttaatct cccatgttct 13201 ggaagtatag gtatcactac acctagctgt attttcatat tttaaaaaaa acgcctaaga 13261 attgctattg aatgaggcgg agagatggct cagcagtcac aagtacttgt agaggactgg 13321 ggtttggtta ctagcacctc aaagatggct tacaattatc tgtagctcca attccagagg 13381 atctgtgtgg taaagctgca tgtatatggg catgcaggta aaactgctaa acatataaaa 13441 atagctcagt cttaagagaa ttggtcgact ttttcgccct ttgacgttgg agtccacgtt 13501 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 13561 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 13621 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 13681 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 13741 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 13801 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 13861 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 13921 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 13981 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 14041 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 14101 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 14161 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 14221 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 14281 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 14341 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 14401 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 14461 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 14521 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 14581 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 14641 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 14701 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 14761 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 14821 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 14881 ccgctaccag gtcgagtagg gataacaggg taatggatcc tcgttgggca cctgcactca 14941 tgtacgcagg cccacatata gatgtacaca cgcatatcat tcaaaataga aattatttat 15001 acaagtagtg cgttggagag ctggctcagg agttgttaat gcttgctacc cttgcaaagg 15061 acccaagttc aatttccagc acctgtgttg ggcatctcac aaccacatgt aaatctagtt 15121 tcaggggatt tcacccctct tctgaactct tcaggcactt atacccacaa atggacatct 15181 ccacatagag acacatactt ttaaaaaagt aatctagggt ctagagaaat ggttatagac 15241 cacggactgg gatcagttcc cagaagccac atcctgtggc tcacaattgc ctgtgaatca 15301 agttctagat ctggtggcct cgtctagcct gcagtcatca acaaacacat gcacacatcc 15361 atacacacat aatttaaaag aaagcgaaag taacctataa tgctaaacaa agcacattct 15421 cagtgtagac tgcagtggtg atgaaccttg aaaatatatt aatagaagcc agacacgtaa 15481 gtcacaaatg gtataactca atttatatga aattcccaga gagtccaaag ttaaaaaaaa 15541 caaaaaggca gattattggt tgtcagtagc tacagagatg ggacattgaa gatattaata 15601 ggtatagggt tgaggattca tggcctttcc ctcttccatg ttagcatgtc tattgttgtt 15661 aaccttattc aactcatgtt agggcaatca tattgatgag acttaatgag tatagcttga 15721 cattcctaga agacagagtc tcacatcaga ctctctgatc ctctgactct tagaatcttt 15781 caacaccctc ttctgaaatg atctctgagc ctttggttgt gactgaatta tgagaaagaa 15841 ttttatgttg aatgaatttt acttcagcat ttttacaaaa ctgaagctgg ggatggcgtt 15901 tcatgcgtgc agtccctacc atttgcctat aagcctggag gaggcagaag tagaattagg 15961 cttttcaggg acatcttcag ctgcatcatg agcttgagga caacttgagc tacataagat 16021 tcccatcccc acccccacac acacacacac atctagtatg tacctagtat gcagtctttt 16081 catatgttta aaatgtagaa aggtatatgt gaagtctccc ttttccccac aacttagtcc 16141 ctccaaagta ttgcatcagt ttccttgtac atgattttat gcatatataa gatacactat 16201 ttctctctct ctctctctcc ttccctcctt ccctccaacc tctgggtatg agttgagggt 16261 tatgtatgtt tcaagttgta ataagaattg ccactcaaac atttcatccc attttaagtt 16321 aagtttgtct gattttccac tttagaaggg cagccatgat ggctcatgcc tataatccca 16381 tcctagcact tgtaagccta atacataaga ctggaggatt gtcatcagct tgaggctagc 16441 ctggtctacc taatgagttc cagccagcca agagtagaat ataagaccct gtctccaaaa 16501 aatgtaccaa aggggctgga ggggtaactc agtggttaag aacagtagct gctcttccag 16561 aggacctggg ttcaattccc agcacccaca tggcagcttg caactatctg taactgtcca 16621 gctttgggat atccgacacc attataccaa ttacataaaa tgaaaaaatt gtaaaatgtg 16681 ctaaaatgag tggggagggg gagcatggtg tggttctgtg ggcttgggat tttgccatga 16741 atccatgact atatctcagc aatatgtgga ttatttctgg aaacctgtgg ggcatgggtg 16801 tatgggaaga ggggagagag agagagcccg catccagcca gagttcctgt gctctgggca 16861 ggcggacgtg ggaggactgc tggacgattt ccacttggcc ctgggtgggc atctggctgt 16921 gtgaagccac tgaccccact cagcgggggg tagataaggg gcatttccca taccaggttc 16981 tcagactcca gcattccacg ctggatacag cagaagagct gagaacagaa taggggcccc 17041 agggagacac tctcgtccca gagataaaga aggcagaggg agagagattc ctacgcaggc 17101 gagaggcctt agtctgggcc ttgatgaaca gagacggtct atggttttag agctttattg 17161 tagaaaggca gggggaaaga gagaaggtag aaagagagag accagccatg gccaagagga 17221 gagaaggggg gaaaggagag agagaagaaa ggctagagag taagaaaggt taaagagaca 17281 gaagtaaaag cttagagtaa gagagagaga gagagcaaga gggagagtaa gaaaagtaaa 17341 agcttaagag agcgaggagg ggccagcccc tctaatagtg ggcttgttat cttgttgttg 17401 ctaggtaact gtgaagcgtc tagcctgaag gccagaagct tgggacattg tctatgtgac 17461 tgaaagccat gagcctctac tgtgggggct gtggaggtgg taacttggac aggagccagg 17521 ggttccagga gacatgaggg aatgccttct gtcccatgta ggtaggaatt accaccaccg 17581 gctttcaccg tagttcactg gagactgttt gtacatagcc cattgcccca cagaaaccag 17641 gcactcactg tttcatttgc ataagaatta gaaggcttta ttttggctgg gattggtgac 17701 ttgccccaga aattccagct tttgggaagc cgaatcctgg agaaccagga gttcaaggtc 17761 atcctcaggt acctcatttg ttcaagacca gcctgtgcta ccatgagacc ttgtccctac 17821 tcccctcaac cccaaaacac gctgttttca ttttataggt tgtactaacc atggaggatg 17881 gtcatttgcc tttctcttga tttcagaagc gtagcgcggg ctttgcacca tgatctagtg 17941 tttccagata ggcttgcctc tccacagcca ggcttatcct gcttcagtcc catgtttggc 18001 aagggcaaca tatgactgaa gaacttgcat agccttatgg tgtatcatga ctcttagccc 18061 cagtttataa ctgtctaaca gctggtggct tttgtgaaaa aggaaagcat catttgaact 18121 tttaactttt cagctgtttg ttttgacttt gcataaatac tgagactagc taggaaatga 18181 gcttttgttt catttgctaa aaatgatttt tttttcatga ggcaatttca agcaatttga 18241 atttagcaat ctctacttgt ctgaaaagaa gttattaatt aaaaaaaaac aaacaaacag 18301 gaacctatta tgctggctag tcacatgaaa gaatccaaat gaacacagtc aaggctgaat 18361 caacacatga aattattttc taattcccca aattacattt atgctaatta gtatgtagta 18421 aaacatttta attgtttcca atgttaaaca ttacagaaat aattttgatg ttttcctaat 18481 aacccagcct ttgatcatgt ttgcatttat ttgattgttg tcagtttatt aaataattaa 18541 gtaagagcaa caagataaaa tgacttcatg agagtccttt tctgtaactt agaacgtcag 18601 tagtcatagg aactgcggtc gttcccttac ctttcagaag taggctaaag agttgaacgc 18661 aaaggtggtt tttcgaatct tcctgatcct taagccccca ctaggtttat attctatatt 18721 tgatatcgga ttcgaacccc tcgagcttag gatccggaac ccttaatata actaagttat 18781 taggtccctc gaagaggttc actagtactg gccattgcgg ccgcttcgag gctgcctcgc 18841 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 18901 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 18961 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 19021 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 19081 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 19141 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 19201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 19261 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 19321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 19381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 19441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 19501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 19561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 19621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 19681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 19741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 19801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 19861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 19921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 19981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 20041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 20101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 20161 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 20221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 20281 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 20341 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 20401 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 20461 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 20521 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 20581 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 20641 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 20701 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 20761 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 20821 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 20881 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 20941 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 21001 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgg //