LOCUS Exported 1029 bp ds-DNA linear SYN 02-JUL-2021 DEFINITION NLSPUFc pCR8 gateway donor vector for insertion of N-terminal domain (SgrAI or AgeI with ClaI) and C-terminal domain (FseI PacI). Grow in dcm- cells (e.g., NEB C3040) to prepare vector for ClaI digest. ACCESSION . VERSION . KEYWORDS pAC1408-pCR8-4xNLS_PUFc_2xNLS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1029) AUTHORS Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, Taghbalout A, Wang H TITLE Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. JOURNAL Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. PUBMED 26768771 REFERENCE 2 (bases 1 to 1029) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 2, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1029 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 12..111 /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS complement(193..213) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(217..237) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa 61 ttgatgagca attatttttt ataatgccaa ctttgtacaa gaaagctggg tcgaattctt 121 aattaatcag gccggccctg atcctccacc tccgcttccg ccgccaccgg aaccgcctcc 181 cccgcggccg cctacctttc ttttcttttt aggatccact ttgcgcttct tctttgggtc 241 ccctaagtca acaccgttct tcatgtagta cttctccagc ttggccagaa tgtgcttgcc 301 ataggtgtac ttacgaagag ttgcgatgtg gggccggatc ttatgcatga cgatcttccg 361 ctggcctggc tccgccacgt caatcatctt ctggaccacg tagttggcat actggtcctt 421 catcatggtg tataaggcac tgtggggacc gtcgttcatg gtgcacacct cgtcgatgag 481 cacagcgcgc tccgtacgtg aggcgtgagt aacacacttc tgcacaacat tgtttgcaaa 541 tttgtgctga ctcaatacaa gtacattgcc tcggatttct gctacaattt tgcttttatc 601 ctcaggacga ccgtgctcca gtacatgttc gattacataa cttccatatt gatcctgtac 661 cagctgctct gtgtgctggt gaagctcctc taaaataggg agtgtctggt cagggagaca 721 gtgctccagg attctctgaa tcactcggca gccataagga tgtgtggata aggcaaatac 781 ctgtccctta aacgcgtcga tgataaattg caaagactgg ggctgtacac attcaatgca 841 tttctgaacc acgtgattgc cattctgatc tttcacacac ttcaagacat ggccatctag 901 ttcccgaacc atctcattct gctggtctga aggaataaac tcaagagctt tctcgataac 961 acggctgcca tacatctgta gtgccaatga caggacgtgg cctcgaatcc gttctgccaa 1021 agccagctt //