LOCUS Exported 20484 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428). ACCESSION . VERSION . KEYWORDS pEMS1626 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 20484) AUTHORS de Leeuw CN, Dyka FM, Boye SL, Laprise S, Zhou M, Chou AY, Borretta L, McInerny SC, Banks KG, Portales-Casamar E, Swanson MI, D'Souza CA, Boye SE, Jones SJ, Holt RA, Goldowitz D, Hauswirth WW, Wasserman WW, Simpson EM TITLE Targeted CNS Delivery Using Human MiniPromoters and Demonstrated Compatibility with Adeno-Associated Viral Vectors. JOURNAL Mol Ther Methods Clin Dev. 2014 Jan 8;1:5. PUBMED 24761428 REFERENCE 2 (bases 1 to 20484) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..20484 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 49..67 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" intron 1794..1926 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" regulatory 1976..1985 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1994..5041 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" primer_bind complement(1996..2018) /label=M13/pUC Forward /note="In lacZ gene" protein_bind 5045..5091 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." polyA_signal 5214..5295 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5251..5270) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12940..12961 /label=F1ori-F /note="F1 origin, forward primer" promoter 13124..13228 /gene="bla" /label=AmpR promoter CDS 13229..14089 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(18296..18318) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 18404..18544 /label=bom /note="basis of mobility region from pBR322" primer_bind 18459..18478 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(18559..18576) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(18730..19318) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(18810..18829) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(19489..20349) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(20350..20454) /gene="bla" /label=AmpR promoter ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctctca gcagattgaa gaagccctcc tggtctgggg agcccgcctg gggacagact 181 cgctcagtct ggttggccct tcagcttggg ggcccctccc caaacttcct agccagcttg 241 ctcacaccct gaccccgggg cctgccgtcc ccacttcctc agctctcacc atggtccccg 301 ccgatcctct ctgcagagct gttctgaatg agacatgagt ctccttccca atcccggccc 361 ccgccccagg ggccctggcc agcagtgcca cttcacgtgg taccgcttca agggacaggc 421 tccgatgcgt gtcccgccgt cctagattgg ggcctgatga gtgtggcctg ggagctggga 481 cacgaatcag ggaaacatgg cccaggagct acccccaggt cccagcatct cccatcaata 541 ggggtccaca cggagagccc tgccctctgc cctggggcct ggcactcaga cccccaagcc 601 caccagcccc tttctacagc cacaactggg tcagggggtc ctaggagact cactcgttaa 661 ttaggtgccc tacaaactaa ttagtcttgt caatcatggg ctctgagacc ttgagctggg 721 ggtggggtgg gggcagggcc ctctcacctc ggcacagggg cctgagcctt cctccgtctt 781 ctcctcctga tccggacact tcattggcat agagggagag agtgtgaact tggccctttg 841 tggaacagag gaggctcggg cagaggtggt gatagtgcag cccattcatt ctgagatgaa 901 acttccactg gtttccgtaa agacgtcttg gggagggaag ggaaggggat ggggacctcc 961 cagtggtatc ccctgcttgg gcactgaggg aaagccacag tggctcgggg taaaaggcag 1021 ggacatcctc tccccgcctg cctctgtccc cagggagtct cgcctcctgt tcccacctgg 1081 ggctagggtg atagaggaga ggagatagct caacctggca tttaggtggt gtgggaacag 1141 gagaccccag actttcttgt tttggggtct ggggcaggca accaggctcc agggacagtg 1201 agttgaagga agggtggctg ggagacccct tgacttgctg ccaaggagac agagctggag 1261 ctagggtggc gggtggtgtc tgaggcaggt gcagagaggg agggagggaa ggggcctttg 1321 actccaacct cctttttctt taccgactgc aggtggcagc tgcccttcca ggagccagtg 1381 ggggaacctg ggtggctggg tggggacacc tgcaagtcct ccctaagcca gctaccaccc 1441 tacactgttg gcctcccttc tccaactgtg gggacgctgc tcaggccttt tgtgacatca 1501 cacctgagag tccctggggt ccagtcattg ctgctgggca cagcgaggtc caagctcagg 1561 tcgccctgcc ccctacccac catgccagat ccagcatcgt tgtgggcaaa caattatctg 1621 gatgatcttt atggggctta agcttgggtg ggagcagatg gggcatgagc tggggatttg 1681 gggatggggg gaatccacac ccccacgtcc tggacgttta aaaggccctc tctggcactg 1741 ggccggggca gaggccagca gaaaagtgac tggagtccaa cgcgtggcgc gccgtaagta 1801 tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa 1861 gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt gcctttctct 1921 ccacaggaag ttcctattcc gaagttccta ttcttcaaaa ggtataggaa cttccgccac 1981 catggcggat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 2041 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 2101 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc 2161 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt 2221 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac 2281 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc 2341 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga 2401 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga 2461 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct 2521 cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg gaagatcagg atatgtggcg 2581 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga 2641 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt 2701 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga 2761 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg 2821 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga 2881 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga 2941 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct 3001 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg 3061 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt 3121 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg 3181 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa 3241 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt 3301 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca 3361 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt 3421 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc 3481 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct 3541 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa 3601 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg 3661 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc 3721 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa 3781 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca 3841 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt 3901 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc 3961 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga 4021 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa 4081 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga 4141 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga 4201 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt 4261 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt 4321 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa 4381 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca 4441 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca 4501 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat 4561 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct 4621 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga 4681 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga 4741 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt 4801 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca 4861 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct 4921 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc 4981 ggcggaattc cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata 5041 ataaaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt cagcggccgc 5101 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 5161 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 5221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5281 catttttttc actgcgaatt ctcgtaaaac tcttcatggc agtagttatt attctctctc 5341 tctctctttt tcttttttct tgagacagga tatttctctg ttgcccaggc tggagtgcag 5401 tggcacagtc ttggctcact gcagcctgga cctcctgggc tcaagccatc ctcccacctc 5461 agcctcccaa gtagctgggg ctacaggcac atggccacca ggcccagata atttttcatt 5521 tttgtagaga ctgagtctca ccatgttacc caggtttatt attctcattt tttagatgaa 5581 gagactgagg tccagagaag ctcaatgact tgcctagttt tacaaatctc ctgccatcac 5641 atacccctca gcgtccttaa taagagggag gccaccaact atgtgctggg cactgtggtg 5701 gatgctggag ctatagggtt gagtatataa gaaatggtgt tgctggagca actgttgctt 5761 gcttacctga cctatctgag aattaattag caggggaaca tatttttgtt ttcagattca 5821 atataagaac ttgtgtgggc aaaaataaag atcagtagta ataacagtag ttcccatttg 5881 ctgactgtac tgtcctaagt gcatatatat atacatacac acacgcatac ctatactcct 5941 ctaatactca aaatgatcct gtttatgtat tgttaatatg ctcattttat ttttaaattt 6001 ttatttattt ttatttttat ttatttttga gacggagtct cattctgtcg cggaggctga 6061 agtgcagtgg tgcgatctca gctcagtgcg acctccgcct cccgggttca agtgattctc 6121 ctgcctcagc ctccggacta gctgggatta caggcgcccg cctccacgcc cagctaattt 6181 ttgtattttt agtagagatg gggtttcgcc atgttggcca ggctggtctc gtactcctga 6241 ccttgagtga tccacctgcc tcggcctccc aaagtgctgg gattacaggc atgagccacc 6301 gcgccgggct aatatgctca ttttagtgag gcaaaaatag aggctcagag tctgatttgt 6361 acaaaactac agagcagtta agtgtcctct cagatgtgta ccctgatctg ggtgactcta 6421 ggactctagg tctcaactgt tacaaccagt taagggtttg gggaagcact gggccaagag 6481 tcaggaaaat ggaagccaca ggtagtgcaa ggtcttggga atgggacgtc tggtccaagg 6541 attcacgcga tgactggaac ccgaagagcc ggggcccggt ttacggccgc catgaagcaa 6601 cgcgcgccgg taggtttggg aatcagggag ccctctgaat aggagactga gttgggaggg 6661 aaaggggctt cgctggggga gcctcggctt cttctgggag aaaattccca cggctaccta 6721 gtgagcctgc aaactggtag gcgccggcgt aggcgcgcgg gcggggccgg gggcggggcc 6781 tgcggggcgt ggcggggcgg gcagagggcg gggcctgctt ctcctcagct tcaggcggct 6841 gcgacgagcc ctcaggcgaa cctctcggct ttcccgcgcg gcgccgcctc ttgctgcgcc 6901 tccgcctcct cctctgctcc gccaccggct tcctcctcct gagcagtcag cccgcgcgcc 6961 ggccggctcc gttatggcga cccgcagccc tggcgtcgtg gtgagcagct cggcctgccg 7021 gccctggccg gttcaggccc acgcggcagg tggcggccgg gccctgaggc gcgggatcga 7081 tccatgacac ccccccgccc cccatatctc actataattc actgtcaaat ggcttttatg 7141 atttggagtt tggggatact tttaaagaca gggtcttggt agctcctaga tggcttggga 7201 ttcacttagt aacccttgct ggcctaaaat tcaggaactt ctacctcatc ttcccaagta 7261 ctgggttagt agggatgtcc agcatattta gcttgcttta atgattttgc tgtacttttt 7321 tctgtgtgta caggtgcatg tatatttata aaactaacat aatatatttt acccccaaag 7381 taccataacc ctaattaatg ctattaaatg ttttgtatgc tgggctctgg gcatactaat 7441 ataaccaata ttatacaatg caaaacaatt ttggaatatc ttctgtaaaa ttatcttatt 7501 agtaagttgt aaccatctca gacttctaag tttgagtttg taaaggatca gttttttttg 7561 gtgacctaca cacacacaca cacacacaca cacacacaca cacacagggg ggtggattct 7621 gagaatttag agaatttaga tggttaatac cagtttttat ttttattctg tgtgatattt 7681 atttattaag atttatttat ttattacatg taggtacact gtatctgtct tcagacacac 7741 cagaagaggg catcagatct cgttacagat ggttgtgagt caccatgtgg ttgctgggat 7801 ttgaactcag gacctctgga agaacagtca gtgctcttaa ccgttgagcc atctcaccag 7861 ccccgtgata ttttatttaa cttactagaa tatactgtgt taaaacatat tttaggggat 7921 tggggagatg gcccattctg taaagtgtct gccatgtcat cttcaggacc tgcgtttgta 7981 accccagcat ggagaagaca gagacaggga gacccctggg cttcctggca agctagacta 8041 gcaaatttgg ttaactaggt tcaatgagag actcagtctc aaaacataag aagtgaattt 8101 tttagaccca atttcaatct ctgaactcca tacgtatgca catgtacctg catataaata 8161 tatatacaca cccataaaca catgcacaca caaaaaatgg ttcaaaagtt cttaaaatgt 8221 ataagagtag aaagtgtaga gccgggagtg gtggcgcacg cctttaatcc cagcacttgg 8281 gagcagaggc aggtggattt ctgagttcga ggccagcctg gtctacagag tgagttccag 8341 gacagccaga gctatacaga gaaatcctgc ctcaaaaaac caaaaataaa gtgtatatac 8401 taatctttca tgcctaaaat atttattttt gccacatttt tcatacctct ttaatatttt 8461 tctttttctc tttgtgcttc aaaggaaatt ttgtgtcatg ttttactttt cattcttgac 8521 tctattcagc agtaagacgc agcatggctt tcttagagaa gtataatcta ttacaaaacg 8581 gtaacattta actgctctac ataccctctt gtagctaaat tcctctgtta aactaagact 8641 tattatctaa acgtaattaa atgttgatat cttataatgc tccactttga aacagctgtc 8701 tttcttttgc agattagcga tgatgaacca ggttatgacc tagatttgtt ttgtatacct 8761 aatcattatg ccgaggattt ggaaaaagtg tttattcctc atggactgat tatggacagg 8821 ttagtaagac ctcgattgaa gttattttta gagggtgtgt gtgtggtgtg tgtgtgtgtg 8881 tgtgtgtgtg tgtgtgtgtg tgtgtaagag agagaggaga ggcatgtgtg atatatatgt 8941 gaagtgtatt gcatgtacat ggaatccaga gaaggacatt aggtgtccta ctctgttgct 9001 ctcttattcc cttgaatcag tgtctcttgc tgaacctggg gcatacatac tgatttggct 9061 aggctgcctg gctagcaaga cccagggatc tgcttgtctc tgccccccag tgctgacgtt 9121 acaagcaggt gcaaccacat ctggttttta tatgggtact gggggatctg aacttagggc 9181 ctgatgcgtg cccaagtgct ctcacccgcc aagccacctc tccagccaaa gggttttttt 9241 tcttgtatta attctaactt agcaactgtt gagtagttga tgtttgaata taagctaagc 9301 caacttatta gaataaatgt tttctatttt ttacttgtat gtgtgagtgc ctgcatgtat 9361 gtatgtgaag catatgtatt ccaggatgtc agaattgtga tggattccct ggaactggag 9421 ttacagacca ttgtgaacca ctgtgtatgg gtgctgggaa ctgaacttta ggtcctctgc 9481 aaaaacagta aggatcttaa ctgataaact atctttctag ccctaaggct ttctactttt 9541 aaagttcgtt tctctttctc tctctctctc tctctctctc tctctctctc cctctccctc 9601 cctcctcctc ctcctcctct ttcccccctc tcgcctcctc tttctctctc ccctccctct 9661 ctctctctca cacacacaca cagacagaga gaggagagag aaaggaagcc ttactaatta 9721 attacctagt tttagacata actagtggga atccatagta agtaaatcaa cattcttgaa 9781 gagaggttag aagaattggc tctcgttgtc catcgaggct gtataagatt attcttctgt 9841 tttctcagct taccatacat attttctacg tagacaatac caattcaaat acactgtacg 9901 taaagtatat gcgcacacac acacacacac acacacacac acacacacaa gttacatatt 9961 gtcatatagt actaaaattt atggactttt tcatattaat acatctagag ctatcatgtt 10021 ttttatcttt taaaaattac tttacggggc tacagagatg tctcagcagt taggagcact 10081 gactgctctt ccagaggtcc tgagttcaat tcccagcaac cacatggtgg ctcacaacca 10141 tctgtaatgg gatctgatcc actcttatgg tgtgtctgaa gacagtgaca gtgtactcat 10201 atacatgaaa taaatcttta aaaaattact ttaccatcag ggtatatata tatatatata 10261 tgtgtgtgtg tgtgtgtgtg tgtatgaagt atttgctatt tgagagggtc tcactgttta 10321 gctcagtctg gcttggaacg ttctgtatag cccagactgg cctcaactca gcagtccccc 10381 tgaaaggctc tcatatgtgg agattacagg tatgtgctac tgtgcccagc cctacatgtt 10441 actttgtttt tgaaataggg tatcaaatag cttagactgg cttctaagtc agtgtgtagc 10501 taaggatggt ctgagcttct gaacctgcta cttcaactcc tggtgtgcca tcatggccag 10561 tttgcacagt tgtgagagta gaactcagga cctcctatgt gttactagac atgttctcta 10621 ccagctgagc tacatcccaa ttcgcctaca tttactttta ttttttaaaa gatttattta 10681 tgtatacagc actctgcctg catgtacatc tgcatgccag aagagggcac cagatctcat 10741 catacatagt tgtgagccac catgtggttg ctggggattg aactcagggc ctctggaaga 10801 gcagtcagtg ctcttaacct ctgagccatc tctgtagccc cctacattta ttttaaagat 10861 tataggctat tctattcata gatgtgccat aactgattta accagatctt cattattttg 10921 tacttagtag agtatattag agggaatgaa tcttaaatac acattttgac acttgctcag 10981 ctttagacaa gtaaaaatca ctggtcaagg aaatggtgct tagtggatgc ttctgaactg 11041 tcaaattgtc ctccaacaag ttacatcatt ttatacttcc aacgttatgt gagagttcat 11101 ttttctttag gctcttctag tattttatat ttgagaaata aaatttaacc aaaatttgcc 11161 aatctagtaa gcttgttttt atttgtatct ttaaatttta tgatagatgt tatagtgtac 11221 tctcctctcc cctcccctcc cctctcctcc tcagcaggat cttgttatgt agccctaagg 11281 gatctaatac ttactctgta gtccaggctg tccttcagct catgagacac accatcatgc 11341 ttggcatgtt tcttgttctg aaaggtatct ggttttaatt ttggttgagc tggtgtgttg 11401 gtgtatatct gtaatctcag cacaggcaag tagagacaag gattatgatt acagtccagc 11461 ctgggctatt tagagggatt ctctttaatc tttcctcatg ccccaaaatc ttacctttgg 11521 tatatgaaaa atagtctcca cttctgcaaa atattgcttt atgaagtaag aattaattcc 11581 cttcatagag acaaggaatg tgtcctgtaa aagtttaatg tgtaagaagt atttgttata 11641 aaagataaat attcagaatc ttctttttaa ttcctgattt tatttctata ggactgaaag 11701 acttgctcga gatgtcatga aggagatggg aggccatcac attgtggccc tctgtgtgct 11761 caaggggggc tataagttct ttgctgacct gctggattac attaaagcac tgaatagaaa 11821 tagtgataga tccattccta tgactgtaga ttttatcaga ctgaagagct actgtgtaag 11881 tataattaac ttataattaa aaaaataggg ccattctagt tttatctata ttttttttaa 11941 acttgtgcaa actatgctac ataatcaact tacttgatta tgtaatatat atagtaatag 12001 ttggaattac tattattaca tatttatttt tctgtgctgg ggtagaaccc agggaactaa 12061 gtactgtaat aaccgagctt cattcatcct agtcattttt aatttttttt tttttttttg 12121 ttttttcgag acaaggtttc tctgtgtagc cctggctgtc ctggaactca ctctgtagac 12181 caggctggcc tcgaactcag aaatctgcct gcctctgcct cccagtgctg agattaatga 12241 cgtgcaccac cactgcctgg cattttttta aagttttaat ttgagaccct ttctcactaa 12301 cttgctcaca ctatcctcga actcattacg tgggctggtc tagaaatttc agtcaacttg 12361 cctcagcttc tcaagtagct gggattacag gcaggtgcta ccacttctgg ccatgatcat 12421 tgttgttgtt gctattatta ttattattat ttcactgata cttcatatca caacacagaa 12481 tagtaactgt agttcatgac caacaaaaaa gttgtacagt ttctctgttg tcttattttg 12541 atctggattt tgatttgttt catggcactg ggcaggctaa tctcaaactg catggctcaa 12601 atagtccttc tgtcttaatc tcccatgttc tggaagtata ggtatcacta cacctagctg 12661 tattttcata ttttaaaaaa aacgcctaag aattgctatt gaatgaggcg gagagatggc 12721 tcagcagtca caagtacttg tagaggactg gggtttggtt actagcacct caaagatggc 12781 ttacaattat ctgtagctcc aattccagag gatctgtgtg gtaaagctgc atgtatatgg 12841 gcatgcaggt aaaactgcta aacatataaa aatagctcag tcttaagaga attggtcgac 12901 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 12961 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 13021 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 13081 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 13141 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 13201 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 13261 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 13321 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 13381 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 13441 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 13501 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 13561 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 13621 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 13681 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 13741 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 13801 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 13861 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 13921 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 13981 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 14041 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 14101 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 14161 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 14221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 14281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca ggtcgagtag ggataacagg 14341 gtaatggatc ctcgttgggc acctgcactc atgtacgcag gcccacatat agatgtacac 14401 acgcatatca ttcaaaatag aaattattta tacaagtagt gcgttggaga gctggctcag 14461 gagttgttaa tgcttgctac ccttgcaaag gacccaagtt caatttccag cacctgtgtt 14521 gggcatctca caaccacatg taaatctagt ttcaggggat ttcacccctc ttctgaactc 14581 ttcaggcact tatacccaca aatggacatc tccacataga gacacatact tttaaaaaag 14641 taatctaggg tctagagaaa tggttataga ccacggactg ggatcagttc ccagaagcca 14701 catcctgtgg ctcacaattg cctgtgaatc aagttctaga tctggtggcc tcgtctagcc 14761 tgcagtcatc aacaaacaca tgcacacatc catacacaca taatttaaaa gaaagcgaaa 14821 gtaacctata atgctaaaca aagcacattc tcagtgtaga ctgcagtggt gatgaacctt 14881 gaaaatatat taatagaagc cagacacgta agtcacaaat ggtataactc aatttatatg 14941 aaattcccag agagtccaaa gttaaaaaaa acaaaaaggc agattattgg ttgtcagtag 15001 ctacagagat gggacattga agatattaat aggtataggg ttgaggattc atggcctttc 15061 cctcttccat gttagcatgt ctattgttgt taaccttatt caactcatgt tagggcaatc 15121 atattgatga gacttaatga gtatagcttg acattcctag aagacagagt ctcacatcag 15181 actctctgat cctctgactc ttagaatctt tcaacaccct cttctgaaat gatctctgag 15241 cctttggttg tgactgaatt atgagaaaga attttatgtt gaatgaattt tacttcagca 15301 tttttacaaa actgaagctg gggatggcgt ttcatgcgtg cagtccctac catttgccta 15361 taagcctgga ggaggcagaa gtagaattag gcttttcagg gacatcttca gctgcatcat 15421 gagcttgagg acaacttgag ctacataaga ttcccatccc cacccccaca cacacacaca 15481 catctagtat gtacctagta tgcagtcttt tcatatgttt aaaatgtaga aaggtatatg 15541 tgaagtctcc cttttcccca caacttagtc cctccaaagt attgcatcag tttccttgta 15601 catgatttta tgcatatata agatacacta tttctctctc tctctctctc cttccctcct 15661 tccctccaac ctctgggtat gagttgaggg ttatgtatgt ttcaagttgt aataagaatt 15721 gccactcaaa catttcatcc cattttaagt taagtttgtc tgattttcca ctttagaagg 15781 gcagccatga tggctcatgc ctataatccc atcctagcac ttgtaagcct aatacataag 15841 actggaggat tgtcatcagc ttgaggctag cctggtctac ctaatgagtt ccagccagcc 15901 aagagtagaa tataagaccc tgtctccaaa aaatgtacca aaggggctgg aggggtaact 15961 cagtggttaa gaacagtagc tgctcttcca gaggacctgg gttcaattcc cagcacccac 16021 atggcagctt gcaactatct gtaactgtcc agctttggga tatccgacac cattatacca 16081 attacataaa atgaaaaaat tgtaaaatgt gctaaaatga gtggggaggg ggagcatggt 16141 gtggttctgt gggcttggga ttttgccatg aatccatgac tatatctcag caatatgtgg 16201 attatttctg gaaacctgtg gggcatgggt gtatgggaag aggggagaga gagagagccc 16261 gcatccagcc agagttcctg tgctctgggc aggcggacgt gggaggactg ctggacgatt 16321 tccacttggc cctgggtggg catctggctg tgtgaagcca ctgaccccac tcagcggggg 16381 gtagataagg ggcatttccc ataccaggtt ctcagactcc agcattccac gctggataca 16441 gcagaagagc tgagaacaga ataggggccc cagggagaca ctctcgtccc agagataaag 16501 aaggcagagg gagagagatt cctacgcagg cgagaggcct tagtctgggc cttgatgaac 16561 agagacggtc tatggtttta gagctttatt gtagaaaggc agggggaaag agagaaggta 16621 gaaagagaga gaccagccat ggccaagagg agagaagggg ggaaaggaga gagagaagaa 16681 aggctagaga gtaagaaagg ttaaagagac agaagtaaaa gcttagagta agagagagag 16741 agagagcaag agggagagta agaaaagtaa aagcttaaga gagcgaggag gggccagccc 16801 ctctaatagt gggcttgtta tcttgttgtt gctaggtaac tgtgaagcgt ctagcctgaa 16861 ggccagaagc ttgggacatt gtctatgtga ctgaaagcca tgagcctcta ctgtgggggc 16921 tgtggaggtg gtaacttgga caggagccag gggttccagg agacatgagg gaatgccttc 16981 tgtcccatgt aggtaggaat taccaccacc ggctttcacc gtagttcact ggagactgtt 17041 tgtacatagc ccattgcccc acagaaacca ggcactcact gtttcatttg cataagaatt 17101 agaaggcttt attttggctg ggattggtga cttgccccag aaattccagc ttttgggaag 17161 ccgaatcctg gagaaccagg agttcaaggt catcctcagg tacctcattt gttcaagacc 17221 agcctgtgct accatgagac cttgtcccta ctcccctcaa ccccaaaaca cgctgttttc 17281 attttatagg ttgtactaac catggaggat ggtcatttgc ctttctcttg atttcagaag 17341 cgtagcgcgg gctttgcacc atgatctagt gtttccagat aggcttgcct ctccacagcc 17401 aggcttatcc tgcttcagtc ccatgtttgg caagggcaac atatgactga agaacttgca 17461 tagccttatg gtgtatcatg actcttagcc ccagtttata actgtctaac agctggtggc 17521 ttttgtgaaa aaggaaagca tcatttgaac ttttaacttt tcagctgttt gttttgactt 17581 tgcataaata ctgagactag ctaggaaatg agcttttgtt tcatttgcta aaaatgattt 17641 ttttttcatg aggcaatttc aagcaatttg aatttagcaa tctctacttg tctgaaaaga 17701 agttattaat taaaaaaaaa caaacaaaca ggaacctatt atgctggcta gtcacatgaa 17761 agaatccaaa tgaacacagt caaggctgaa tcaacacatg aaattatttt ctaattcccc 17821 aaattacatt tatgctaatt agtatgtagt aaaacatttt aattgtttcc aatgttaaac 17881 attacagaaa taattttgat gttttcctaa taacccagcc tttgatcatg tttgcattta 17941 tttgattgtt gtcagtttat taaataatta agtaagagca acaagataaa atgacttcat 18001 gagagtcctt ttctgtaact tagaacgtca gtagtcatag gaactgcggt cgttccctta 18061 cctttcagaa gtaggctaaa gagttgaacg caaaggtggt ttttcgaatc ttcctgatcc 18121 ttaagccccc actaggttta tattctatat ttgatatcgg attcgaaccc ctcgagctta 18181 ggatccggaa cccttaatat aactaagtta ttaggtccct cgaagaggtt cactagtact 18241 ggccattgcg gccgcttcga ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 18301 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 18361 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt 18421 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact 18481 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 18541 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 18601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 18661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 18721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 18781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 18841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 18901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 18961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 19021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 19081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 19141 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 19201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 19261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 19321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 19381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 19441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 19501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 19561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 19621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 19681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 19741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 19801 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 19861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 19921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 19981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 20041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 20101 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 20161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 20221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 20281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 20341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 20401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 20461 tccccgaaaa gtgccacctg acgg //