LOCUS       Exported               10602 bp ds-DNA     circular SYN 23-JUN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CMV-GFP-NMHC II-A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Wei Q, Adelstein RS
  TITLE     Conditional expression of a truncated fragment of nonmuscle myosin 
            II-A alters cell shape but not cytokinesis in HeLa cells.
  JOURNAL   Mol Biol Cell. 2000 Oct . 11(10):3617-27.
  PUBMED    11029059
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 23, 2023 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      614..623
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             620..1336
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             620..1336
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(665..686)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(926..945)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1273..1294
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7397..7518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7434..7453)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7488..7507
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7525..7980)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7662..7683)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7874..7893
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8007..8111
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8113..8470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(8136..8156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8321..8456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8383..8402
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8505..9299
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8559..8578)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9169..9188
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9487..9506)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9531..9578
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9907..10495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10396..10415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      661 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      721 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      781 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      841 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      901 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      961 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1021 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1081 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1141 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1201 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1261 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1321 ggacgagctg tacaagtact cagatctcga gctcaagctt atggcacagc aagctgccga
     1381 taagtatctc tatgtggata aaaacttcat caacaatccg ctggcccagg ccgactgggc
     1441 tgccaagaag ctggtatggg tgccttccga caagagtggc tttgagccag ccagcctcaa
     1501 ggaggaggtg ggcgaagagg ccatcgtgga gctggtggag aatgggaaga aggtgaaggt
     1561 gaacaaggat gacatccaga agatgaaccc gcccaagttc tccaaggtgg aggacatggc
     1621 agagctcacg tgcctcaacg aagcctcggt gctgcacaac ctcaaggagc gttactactc
     1681 agggctcatc tacacctatt caggcctgtt ctgtgtggtc atcaatcctt acaagaacct
     1741 gcccatctac tctgaagaga ttgtggaaat gtacaagggc aagaagaggc acgagatgcc
     1801 ccctcacatc tatgccatca cagacaccgc ctacaggagt atgatgcaag accgagaaga
     1861 tcaatccatc ttgtgcactg gtgaatctgg agctggcaag acggagaaca ccaagaaggt
     1921 catccagtat ctggcgtacg tggcgtcctc gcacaagagc aagaaggacc agggcgagct
     1981 ggagcggcag ctgctgcagg ccaaccccat cctggaggcc ttcgggaacg ccaagaccgt
     2041 gaagaatgac aactcctccc gcttcggcaa attcattcgc atcaactttg atgtcaatgg
     2101 ctacattgtt ggagccaaca ttgagactta tcttttggag aaatctcgtg ctatccgcca
     2161 agccaaggaa gaacggacct tccacatctt ctattatctc ctgtctgggg ctggagagca
     2221 cctgaagacc gatctcctgt tggagccgta caacaaatac cgcttcctgt ccaatggaca
     2281 cgtcaccatc cccgggcagc aggacaagga catgttccag gagaccatgg aggccatgag
     2341 gattatgggc atcccagaag aggagcaaat gggcctgctg cgggtcatct caggggttct
     2401 tcagctcggc aacatcgtct tcaagaagga gcggaacact gaccaggcgt ccatgcccga
     2461 caacacagct gcccaaaagg tgtcccatct cttgggtatc aatgtgaccg atttcaccag
     2521 aggaatcctc accccgcgca tcaaggtggg acgggattac gtccagaagg cgcagactaa
     2581 agagcaggct gactttgcca tcgaggcctt ggccaaggcg acctatgagc ggatgttccg
     2641 ctggctggtg ctgcgcatca acaaggctct ggacaagacc aagaggcagg gcgcctcctt
     2701 catcgggatc ctggacattg ccggcttcga gatctttgat ctgaactcgt ttgagcagct
     2761 gtgcatcaat tacaccaatg agaagctgca gcagctcttc aaccacacca tgttcatcct
     2821 ggagcaggag gagtaccagc gcgagggcat cgagtggaac ttcatcgact ttggcctcga
     2881 cctgcagccc tgcatcgacc tcattgagaa gccagcaggc cccccgggca ttctggccct
     2941 gctggacgag gagtgctggt tccccaaagc caccgacaag agcttcgtgg agaaggtgat
     3001 gcaggagcag ggcacccacc ccaagttcca gaagcccaag cagctgaagg acaaagctga
     3061 tttctgcatt atccactatg ccggcaaggt ggattacaaa gctgacgagt ggctgatgaa
     3121 gaacatggat cccctgaatg acaacatcgc cacactgctc caccagtcct ctgacaagtt
     3181 tgtctcggag ctgtggaagg atgtggaccg catcatcggc ctggaccagg tggccggcat
     3241 gtcggagacc gcactgcccg gggccttcaa gacgcggaag ggcatgttcc gcactgtggg
     3301 gcagctttac aaggagcagc tggccaagct gatggctacg ctgaggaaca cgaaccccaa
     3361 ctttgtccgc tgcatcatcc ccaaccacga gaagaaggcc ggcaagctgg acccgcatct
     3421 cgtgctggac cagctgcgct gcaacggtgt tctcgagggc atccgtatct gccgccaggg
     3481 cttccccaac agggtggtct tccaggagtt tcggcagaga tatgagatcc tgactccaaa
     3541 ctccattccc aagggtttca tggacgggaa gcaggcgtgc gtgctcatga taaaagccct
     3601 ggagctcgac agcaatctgt accgcattgg ccagagcaaa gtcttcttcc gtgccggtgt
     3661 gctggcccac ctggaggagg agcgagacct gaagatcacc gacgtcatca tagggttcca
     3721 ggcctgctgc aggggctacc tggccaggaa agcatttgcc aagcggcagc agcagcttac
     3781 cgccatgaag gtcctccagc ggaactgcgc tgcctacctg aagctgcgga actggcagtg
     3841 gtggcggctc ttcaccaagg tcaagccgct gctgcaggtg agccggcagg aggaggagat
     3901 gatggccaag gaggaggagc tggtgaaggt cagagagaag cagctggctg cggagaacag
     3961 gctcacggag atggagacgc tgcagtctca gctcatggca gagaaattgc agctgcagga
     4021 gcagctccag gcagaaaccg agctgtgtgc cgaggctgag gagctccggg cccgcctgac
     4081 cgccaagaag caggaattag aagagatctg ccatgaccta gaggccaggg tggaggagga
     4141 ggaggagcgc tgccagcacc tgcaggcgga gaagaagaag atgcagcaga acatccagga
     4201 gcttgaggag cagctggagg aggaggagag cgcccggcag aagctgcagc tggagaaggt
     4261 gaccaccgag gcgaagctga aaaagctgga ggaggagcag atcatcctgg aggaccagaa
     4321 ctgcaagctg gccaaggaaa agaaactgct ggaagacaga atagctgagt tcaccaccaa
     4381 cctcacagaa gaggaggaga aatctaagag cctcgccaag ctcaagaaca agcatgaggc
     4441 aatgatcact gacttggaag agcgcctccg cagggaggag aagcagcgac aggagctgga
     4501 gaagacccgc cggaagctgg agggagactc cacagacctc agcgaccaga tcgccgagct
     4561 ccaggcccag atcgcggagc tcaagatgca gctggccaag aaagaggagg agctccaggc
     4621 cgccctggcc agagtggaag aggaagctgc ccagaagaac atggccctca agaagatccg
     4681 ggagctggaa tctcagatct ctgaactcca ggaagacctg gagtctgagc gtgcttccag
     4741 gaataaagct gagaagcaga aacgggacct tggggaagag ctagaggcgc tgaaaacaga
     4801 gttggaggac acgctggatt ccacagctgc ccagcaggag ctcaggtcaa aacgtgagca
     4861 ggaggtgaac atcctgaaga agaccctgga ggaggaggcc aagacccacg aggcccagat
     4921 ccaggagatg aggcagaagc actcacaggc cgtggaggag ctggcggagc agctggagca
     4981 gacgaagcgg gtgaaagcaa acctcgagaa ggcaaagcag actctggaga acgagcgggg
     5041 ggagctggcc aacgaggtga aggtgctgct gcagggcaaa ggggactcgg agcacaagcg
     5101 caagaaagtg gaggcgcagc tgcaggagct gcaggtcaag ttcaacgagg gagagcgcgt
     5161 gcgcacagag ctggccgaca aggtcaccaa gctgcaggtg gagctggaca acgtgaccgg
     5221 gcttctcagc cagtccgaca gcaagtccag caagctcacc aaggacttct ccgcgctgga
     5281 gtcccagctg caggacactc aggagctgct gcaggaggag aaccggcaga agctgagcct
     5341 gagcaccaag ctcaagcagg tggaggacga gaagaattcc ttccgggagc agctggagga
     5401 ggaggaggag gccaagcaca acctggagaa gcagatcgcc accctccatg cccaggtggc
     5461 cgacatgaaa aagaagatgg aggacagtgt ggggtgcctg gaaactgctg aggaggtgaa
     5521 gaggaagctc cagaaggacc tggagggcct gagccagcgg cacgaggaga aggtggccgc
     5581 ctacgacaag ctggagaaga ccaagacgcg gctgcagcag gagctggacg acctgctggt
     5641 ggacctggac caccagcgcc agagcgcgtg caacctggag aagaagcaga agaagtttga
     5701 ccagctcctg gcggaggaga agaccatctc tgccaagtat gcagaggagc gcgaccgggc
     5761 tgaggcggag gcccgagaga aggagaccaa ggctctgtcg ctggcccggg ccctggagga
     5821 agccatggag cagaaggcgg agctggagcg gctcaacaag cagttccgca cggagatgga
     5881 ggaccttatg agctccaagg atgatgtggg caagagtgtc cacgagctgg agaagtccaa
     5941 gcgggcccta gagcagcagg tggaggagat gaagacgcag ctggaagagc tggaggacga
     6001 gctgcaggcc accgaagatg ccaagctgcg gttggaggtc aacctgcagg ccatgaaggc
     6061 ccagttcgag cgggacctgc agggccggga cgagcagagc gaggagaaga agaagcagct
     6121 ggtcagacag gtgcgggaga tggaggcaga gctggaggac gagaggaagc agcgctcgat
     6181 ggcagtggcc gcccggaaga agctggagat ggacctgaag gacctggagg cgcacatcga
     6241 ctcggccaac aagaaccggg acgaagccat caaacagctg cggaagctgc aggcccagat
     6301 gaaggactgc atgcgcgagc tggatgacac ccgcgcctct cgtgaggaga tcctggccca
     6361 ggccaaagag aacgagaaga agctgaagag catggaggcc gagatgatcc agttgcagga
     6421 ggaactggca gccgcggagc gtgccaagcg ccaggcccag caggagcggg atgagctggc
     6481 tgacgagatc gccaacagca gcggcaaagg agccctggcg ttagaggaga agcggcgtct
     6541 ggaggcccgc atcgcccagc tggaggagga gctggaggag gagcagggca acacggagct
     6601 gatcaacgac cggctgaaga aggccaacct gcagatcgac cagatcaaca ccgacctgaa
     6661 cctggagcgc agccacgccc agaagaacga gaatgctcgg cagcagctgg aacgccagaa
     6721 caaggagctt aaggtcaagc tgcaggagat ggagggcact gtcaagtcca agtacaaggc
     6781 ctccatcacc gccctcgagg ccaagattgc acagctggag gagcagctgg acaacgagac
     6841 caaggagcgc caggcagcct gcaaacaggt gcgtcggacc gagaagaagc tgaaggatgt
     6901 gctgctgcag gtggatgacg agcggaggaa cgccgagcag tacaaggacc aggccgacaa
     6961 ggcatctacc cgcctgaagc agctcaagcg gcagctggag gaggccgaag aggaggccca
     7021 gcgggccaac gcctcccgcc ggaaactgca gcgcgagctg gaggacgcca ctgagacggc
     7081 cgatgccatg aaccgcgaag tcagctccct aaagaacaag ctcaggcgcg gggacctgcc
     7141 gtttgtcgtg ccccgccgaa tggcccggaa aggcgccggg gatggctccg acgaagaggt
     7201 agatggcaaa gcggatgggg ctgaggccaa acctgccgaa taagtcgact ctaatcacta
     7261 gtgcggccga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     7321 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     7381 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7441 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7501 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     7561 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     7621 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     7681 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     7741 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     7801 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     7861 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     7921 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     7981 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8041 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8101 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     8161 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8221 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8281 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8341 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8401 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8461 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     8521 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     8581 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     8641 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     8701 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     8761 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     8821 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     8881 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     8941 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     9001 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     9061 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     9121 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     9181 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     9241 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     9301 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     9361 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     9421 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     9481 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     9541 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     9601 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     9661 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     9721 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     9781 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9841 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9901 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9961 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    10021 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    10081 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    10141 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    10201 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    10261 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    10321 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    10381 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    10441 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    10501 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    10561 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gc
//
