LOCUS Exported 10081 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS tetO-TBX5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10081) AUTHORS Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, Christoforou N, Epstein JA, Gearhart JD TITLE Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. JOURNAL J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004. PUBMED 23591016 REFERENCE 2 (bases 1 to 10081) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10081 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(63..80) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(234..822) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(314..333) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(993..1853) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1616..1635 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1854..1958) /gene="bla" /label=AmpR promoter primer_bind complement(2033..2052) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 2224..2603 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2605..2803 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2758..2778 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 2821..3001 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 3048..3173 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3666..3899 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 4084..4128 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4426..4543 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 4596..4614 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4638..4656 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4680..4698 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4722..4740 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 4764..4782 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" promoter 4815..4853 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4850..4874 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind 4953..4972 /label=T7 /note="T7 promoter, forward primer" promoter 4953..4971 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 5003..5027 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6591..6615) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 6641..6682 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 6692..6709 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 6759..7347 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6812..6832) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7230..7241) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(7350..7366) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7351..7367) /label=pBluescriptKS /note="For pBluescript vector" LTR 7872..8052 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8078..8095) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8084..8308 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8354..8782 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8441..8460) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8651..8672 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8791..8811) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8796..9125 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8976..9111 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9038..9057 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9173..9220 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9239..9613 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9743..9864 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9780..9799) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9834..9853 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9913..9929) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9913..9929) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9926..9948) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9937..9953 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9961..9991) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10006..10027 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." ORIGIN 1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 1981 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct 2041 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg 2101 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga 2161 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc 2221 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 2281 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 2341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 2401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 2461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 2521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 2581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 2641 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 2701 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 2761 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact 2821 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 2881 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 2941 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 3001 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 3061 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 3121 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 3181 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 3241 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 3301 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 3361 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 3421 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 3481 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 3541 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 3601 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 3661 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 3721 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 3781 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 3841 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 3901 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 3961 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 4021 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 4081 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 4141 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 4201 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 4261 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 4321 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 4381 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 4441 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 4501 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 4561 cagcagagat ccagtttggt tatcgagttt accactccct atcagtgata gagaaaagtg 4621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact 4681 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga 4741 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc 4801 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag 4861 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc 4921 gggaccgatc cagcctccgc ggccccgaat tgtaatacga ctcactatag ggagacccaa 4981 gctggctagg taagcttgat caacaagttt gtacaaaaaa gcaggctcca ccatggccga 5041 cgcagacgag ggctttggcc tggcgcacac gcctctggag cctgacgcaa aagacctgcc 5101 ctgcgattcg aaacccgaga gcgcgctcgg ggcccccagc aagtccccgt cgtccccgca 5161 ggccgccttc acccagcagg gcatggaggg aatcaaagtg tttctccatg aaagagaact 5221 gtggctaaaa ttccacgaag tgggcacgga aatgatcata accaaggctg gaaggcggat 5281 gtttcccagt tacaaagtga aggtgacggg ccttaatccc aaaacgaagt acattcttct 5341 catggacatt gtacctgccg acgatcacag atacaaattc gcagataata aatggtctgt 5401 gacgggcaaa gctgagcccg ccatgcctgg ccgcctgtac gtgcacccag actcccccgc 5461 caccggggcg cattggatga ggcagctcgt ctccttccag aaactcaagc tcaccaacaa 5521 ccacctggac ccatttgggc atattattct aaattccatg cacaaatacc agcctagatt 5581 acacatcgtg aaagcggatg aaaataatgg atttggctca aaaaatacag cgttctgcac 5641 tcacgtcttt cctgagactg cgtttatagc agtgacttcc taccagaacc acaagatcac 5701 gcaattaaag attgagaata atccctttgc caaaggattt cggggcagtg atgacatgga 5761 gctgcacaga atgtcaagaa tgcaaagtaa agaatatccc gtggtcccca ggagcaccgt 5821 gaggcaaaaa gtggcctcca accacagtcc tttcagcagc gagtctcgag ctctctccac 5881 ctcatccaat ttggggtccc aataccagtg tgagaatggt gtttccggcc cctcccagga 5941 cctcctgcct ccacccaacc catacccact gccccaggag catagccaaa tttaccattg 6001 taccaagagg aaagaggaag aatgttccac cacagaccat ccctataaga agccctacat 6061 ggagacatca cccagtgaag aagattcctt ctaccgctct agctatccac agcagcaggg 6121 cctgggtgcc tcctacagga cagagtcggc acagcggcaa gcttgcatgt atgccagctc 6181 tgcgcccccc agcgagcctg tgcccagcct agaggacatc agctgcaaca cgtggccaag 6241 catgccttcc tacagcagct gcaccgtcac caccgtgcag cccatggaca ggctacccta 6301 ccagcacttc tccgctcact tcacctcggg gcccctggtc cctcggctgg ctggcatggc 6361 caaccatggc tccccacagc tgggagaggg aatgttccag caccagacct ccgtggccca 6421 ccagcctgtg gtcaggcagt gtgggcctca gactggcctg cagtcccctg gcacccttca 6481 gccccctgag ttcctctact ctcatggcgt gccaaggact ctatcccctc atcagtacca 6541 ctctgtgcac ggagttggca tggtgccaga gtggagcgac aatagcttgg acccagcttt 6601 cttgtacaaa gtggttgatc tagagggccc gcggttcgaa ggtaagccta tccctaaccc 6661 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac 6721 ccgctgatca gcctcaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa 6781 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 6841 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 6901 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 6961 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 7021 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 7081 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 7141 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 7201 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 7261 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 7321 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac 7381 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag 7441 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga 7501 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 7561 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct 7621 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg 7681 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag 7741 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag 7801 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc 7861 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 7921 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 7981 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 8041 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc 8101 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 8161 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 8221 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 8281 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct 8341 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 8401 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 8461 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 8521 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 8581 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 8641 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 8701 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 8761 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 8821 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 8881 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 8941 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 9001 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 9061 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 9121 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa 9181 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat 9241 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga 9301 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt 9361 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa 9421 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt 9481 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc 9541 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga 9601 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt 9661 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 9721 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 9781 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 9841 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 9901 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 9961 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 10021 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 10081 g //