LOCUS Exported 773 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.. ACCESSION . VERSION . KEYWORDS pET28a-SnoopCatcher SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 773) AUTHORS Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M TITLE Programmable polyproteams built using twin peptide superglues. JOURNAL Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. PUBMED 26787909 REFERENCE 2 (bases 1 to 773) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..773 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 34..51 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 61..78 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" primer_bind complement(518..536) /label=T7 Term /note="T7 terminator, reverse primer" terminator 532..579 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(703..722) /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc 61 ctggtgccgc gcggcagcca tatgaagccg ctgcgtggtg ccgtgtttag cctgcagaaa 121 cagcatcccg actatcccga tatctatggc gcgattgatc agaatgggac ctatcaaaat 181 gtgcgtaccg gcgaagatgg taaactgacc tttaagaatc tgagcgatgg caaatatcgc 241 ctgtttgaaa atagcgaacc cgctggctat aaaccggtgc agaataagcc gattgtggcg 301 tttcagattg tgaatggcga agtgcgtgat gtgaccagca ttgtgccgca ggatattccg 361 gctacatatg aatttaccaa cggtaaacat tatatcacca atgaaccgat accgccgaaa 421 taataaaagc ttgcggccgc actcgagcac taccaccacc accactgaga tccggctgct 481 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa 541 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc 601 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 661 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 721 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggg //