LOCUS       Exported                 592 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  3rd generation lentiviral plasmid for inducible expression of shRNA;
            puromycin selection. See manual for detailed protocols. No further 
            technical support is available from the depositing laboratory..
ACCESSION   .
VERSION     .
KEYWORDS    Tet-pLKO-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Wiederschain D, Wee S, Chen L, Loo A, Yang G, Huang A, Chen Y, 
            Caponigro G, Yao YM, Lengauer C, Sellers WR, Benson JD
  TITLE     Single-vector inducible lentiviral RNAi system for oncology target 
            validation.
  JOURNAL   Cell Cycle. 2009 Feb 1. 8(3):498-504.
  PUBMED    19177017
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        104..198
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     115..134
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
       61 atctcgacgg tatcgatcac gagactagcc tcgagcggcc gcaatatttg catgtcgcta
      121 tgtgttctgg gaaatcacca taaacgtgaa atccctatca gtgatagaga cttataagtt
      181 ccctatcagt gatagagaca ccggtgtgtt gtaaatgagc acacaaaata cacatgctaa
      241 aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt
      301 atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata
      361 aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca
      421 ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt
      481 ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt cattagttta
      541 acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac aa
//
