LOCUS Exported 3153 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS p6xUAS (GB0179) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3153) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3153) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3153 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(204..222) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(205..222) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(240..256) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(240..256) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(253..275) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 264..280 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(288..318) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 333..354 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(471..488) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(642..1230) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(722..741) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1401..2261) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2024..2043 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2262..2366) /gene="bla" /label=AmpR promoter rep_origin complement(2444..2899) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2581..2602) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2793..2812 /label=F1ori-R /note="F1 origin, reverse primer" CDS complement(2876..244) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDWAMSG LGDGGQYSARRTVLRSEDSTPLGGQYSARRTVLRSRDHSSPIEFPRPPWRPGACDVGPN SPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLN GEWTRPVAAH" primer_bind 3025..3047 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3039..3056 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3040..3056 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3063..3082 /label=T7 /note="T7 promoter, forward primer" promoter 3063..3081 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3112..3121 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3116..3125 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgggagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct 61 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gtccccgaga ccactcatcg 121 cccaatcact agtgaattcg cggccgcctg caggtcgacc atatgggaga gctcccaacg 181 cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg 241 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 301 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 361 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 421 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 481 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 541 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 601 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 661 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 721 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 781 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 841 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 901 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 961 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 1021 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 1081 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 1141 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 1201 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 1261 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 1321 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 1381 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 1441 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 1501 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 1561 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 1621 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 1681 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 1741 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 1801 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 1861 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 1921 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 1981 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 2041 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 2101 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 2161 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 2221 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 2281 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 2341 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg 2401 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat 2461 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 2521 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 2581 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 2641 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 2701 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 2761 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 2821 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 2881 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat 2941 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 3001 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat 3061 tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg 3121 gcggccgcgg gaattcgatg ggcgatgagt ggt //