LOCUS       Exported                9326 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses humanized PcCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    pY017 (pcDNA3.1-hPcCpf1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     647..666
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        647..665
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      707..716
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4493..4540
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4547..4627
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4701..4718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4707..4931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4977..5405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5064..5083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5274..5295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5414..5434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5419..5748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5599..5734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5661..5680
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5815..6609
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5869..5888)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6479..6498
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6783..6904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6820..6839)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6874..6893
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6953..6969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6953..6969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6966..6988)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6977..6993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7001..7031)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7046..7067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7184..7201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7355..7943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7435..7454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8114..8974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8737..8756
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8975..9079)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9154..9173)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      661 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgcca ccatggacag
      721 cctgaaggat ttcaccaacc tgtaccccgt gtccaagaca ctgcggtttg agctgaagcc
      781 tgtgggcaag accctggaga atatcgagaa ggccggcatc ctgaaggagg atgagcacag
      841 agccgagagc taccggagag tgaagaagat catcgataca tatcacaagg tgttcatcga
      901 cagctccctg gagaacatgg ccaagatggg catcgagaat gagatcaagg ccatgctgca
      961 gtccttttgc gagctgtata agaaggacca caggaccgag ggagaggaca aggccctgga
     1021 taagatcagg gccgtgctga ggggcctgat cgtgggagcc ttcaccggcg tgtgcggccg
     1081 gcgggagaac acagtgcaga atgagaagta tgagagcctg tttaaggaga agctgatcaa
     1141 ggagatcctg ccagatttcg tgctgtctac agaggccgag tccctgccct tttctgtgga
     1201 ggaggccacc agaagcctga aggagttcga ctcctttaca tcttacttcg ccggctttta
     1261 tgagaaccgg aagaatatct actctaccaa gccccagagc acagccatcg cctatagact
     1321 gatccacgag aacctgccta agttcatcga taatatcctg gtgtttcaga agatcaagga
     1381 gccaatcgcc aaggagctgg agcacatcag ggcagacttc agcgccggcg gctacatcaa
     1441 gaaggatgag cgcctggagg acatcttttc cctgaactac tatatccacg tgctgtctca
     1501 ggccggcatc gagaagtaca atgccctgat cggcaagatc gtgaccgagg gcgatggcga
     1561 gatgaagggc ctgaacgagc acatcaacct gtataatcag cagaggggcc gcgaggaccg
     1621 gctgccactg ttcagacccc tgtataagca gatcctgtct gatagggagc agctgtccta
     1681 tctgccagag tctttcgaga aggacgagga gctgctgagg gccctgaagg agttttacga
     1741 tcacatcgca gaggacatcc tgggaaggac ccagcagctg atgacaagca tctccgagta
     1801 cgatctgtcc cggatctatg tgagaaacga tagccagctg accgacatct ccaagaagat
     1861 gctgggcgat tggaatgcca tctacatggc ccgggagaga gcctatgacc acgagcaggc
     1921 ccccaagcgc atcacagcca agtacgagag ggaccgcatc aaggccctga agggcgagga
     1981 gtctatcagc ctggccaacc tgaacagctg catcgccttc ctggacaacg tgagggattg
     2041 tcgcgtggac acctatctgt ctacactggg acagaaggag ggacctcacg gcctgagcaa
     2101 cctggtggag aacgtgttcg cctcctacca cgaggccgag cagctgctgt cttttcccta
     2161 tcctgaggag aacaatctga tccaggacaa ggataacgtg gtgctgatca agaacctgct
     2221 ggataatatc agcgacctgc agaggttcct gaagccactg tggggcatgg gcgatgagcc
     2281 cgacaaggat gagaggtttt acggcgagta caattatatc aggggcgccc tggaccaggt
     2341 catccctctg tataacaagg tgcggaatta tctgacccgc aagccatact ccacacgcaa
     2401 ggtgaagctg aacttcggca atagccagct gctgtccggc tgggatagga acaaggagaa
     2461 ggacaattct tgcgtgatcc tgcgcaaggg ccagaacttc tacctggcca tcatgaacaa
     2521 tcggcacaag cggagcttcg agaataagat gctgcccgag tataaggagg gcgagcctta
     2581 cttcgagaag atggattata agtttctgcc agaccccaac aagatgctgc ccaaggtgtt
     2641 cctgtctaag aagggcatcg agatctacaa gcctagccca aagctgctgg agcagtatgg
     2701 ccacggcacc cacaagaagg gcgatacctt cagcatggac gatctgcacg agctgatcga
     2761 cttctttaag cactccatcg aggcccacga ggattggaag cagttcggct ttaagttcag
     2821 cgacaccgcc acatacgaga acgtgagcag cttctaccgg gaggtggagg accagggcta
     2881 caagctgtct tttagaaagg tgtccgagtc ttacgtgtat agcctgatcg atcagggcaa
     2941 gctgtacctg ttccagatct ataacaagga ctttagccct tgttccaagg gcaccccaaa
     3001 tctgcacaca ctgtactggc ggatgctgtt cgatgagaga aacctggccg acgtgatcta
     3061 taagctggat ggcaaggccg agatcttctt tcgggagaag tccctgaaga atgaccaccc
     3121 aacccaccct gcaggcaagc ccatcaagaa gaagagccgg cagaagaagg gcgaggagag
     3181 cctgttcgag tacgatctgg tgaaggaccg gagatatacc atggataagt ttcagttcca
     3241 cgtgccaatc acaatgaact ttaagtgctc tgccggcagc aaggtgaacg acatggtgaa
     3301 tgcccacatc agggaggcca aggacatgca cgtgatcggc atcgataggg gcgagcgcaa
     3361 tctgctgtat atctgcgtga tcgacagccg cggcaccatc ctggatcaga tctccctgaa
     3421 cacaatcaat gacatcgatt atcacgatct gctggagtcc agggacaagg atcgccagca
     3481 ggagcacagg aactggcaga ccatcgaggg catcaaggag ctgaagcagg gctacctgtc
     3541 tcaggccgtg caccgcatcg ccgagctgat ggtggcctat aaggccgtgg tggccctgga
     3601 ggacctgaac atgggcttca agcggggcag acagaaggtg gagagcagcg tgtaccagca
     3661 gtttgagaag cagctgatcg acaagctgaa ttatctggtg gataagaaga agcggcccga
     3721 ggacatcgga ggcctgctga gagcctacca gttcaccgcc cctttcaaga gctttaagga
     3781 gatgggcaag cagaacggct ttctgttcta tatccctgcc tggaacacat ccaatatcga
     3841 cccaaccaca ggcttcgtga acctgtttca cgtgcagtac gagaatgtgg ataaggccaa
     3901 gagcttcttt cagaagttcg acagcatctc ctacaaccct aagaaggatt ggtttgagtt
     3961 cgcctttgac tataagaact tcaccaagaa ggccgagggc tctaggagca tgtggattct
     4021 gtgcacccac ggctcccgga tcaagaactt cagaaattct cagaagaatg gccagtggga
     4081 tagcgaggag tttgccctga ccgaggcctt caagtccctg tttgtgcggt acgagatcga
     4141 ttataccgcc gacctgaaaa ccgccatcgt ggacgagaag cagaaggatt tctttgtgga
     4201 cctgctgaag ctgttcaagc tgaccgtgca gatgagaaac tcctggaagg agaaggacct
     4261 ggattacctg atctctccag tggccggcgc cgatggcagg ttctttgaca cacgcgaggg
     4321 caataagagc ctgcccaagg acgcagatgc aaacggagcc tataatatcg ccctgaaggg
     4381 cctgtgggca ctgaggcaga tcagacagac ctccgagggc ggcaagctga agctggccat
     4441 ctctaacaag gagtggctgc agtttgtgca ggagagatcc tacgagaagg acaaaaggcc
     4501 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
     4561 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
     4621 ctatgcctaa gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     4681 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4741 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4801 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4861 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4921 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4981 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5041 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5101 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5161 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5221 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5281 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5341 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5401 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5461 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5581 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5641 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5701 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5761 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     5821 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5881 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5941 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     6001 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6061 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6121 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6181 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6241 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6301 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     6361 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6421 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6481 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6541 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6601 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6661 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6721 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6781 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6841 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6901 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6961 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7021 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7081 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7141 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7201 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7981 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8041 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8101 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8161 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8221 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8281 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8341 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8401 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8461 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8521 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8581 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8641 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8701 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8761 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8821 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8881 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8941 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9001 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9061 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     9121 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9181 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     9241 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     9301 gcgttttgcg ctgcttcgcg atgtac
//
