LOCUS Exported 15841 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSADdeltaG-GFP-F2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15841) AUTHORS Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM TITLE New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. JOURNAL Neuron. 2011 Aug 25;71(4):617-31. PUBMED 21867879 REFERENCE 2 (bases 1 to 15841) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15841 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 4043..4052 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4049..4768 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPNNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4094..4115) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4355..4374) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4702..4723 /label=EGFP-C /note="EGFP, forward primer" CDS 6506..6517 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(11615..11632) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 11621..11845 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 11891..12319 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11978..11997) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 12188..12209 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(12328..12348) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 12333..12662 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 12513..12648 /label=SV40 ori /note="SV40 origin of replication" primer_bind 12575..12594 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 12710..12757 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 12776..13150 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 13280..13401 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(13317..13336) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 13371..13390 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(13450..13466) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(13450..13466) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(13463..13485) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 13474..13490 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(13498..13528) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13543..13564 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(13681..13698) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13852..14440) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13932..13951) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14611..15471) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 15234..15253 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(15472..15576) /gene="bla" /label=AmpR promoter primer_bind complement(15651..15670) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg 661 gctagattaa gcgtctgatg agtccgtgag gacgaaaccc ggagtcccgg gtcacgctta 721 acaaccagat caaagaaaaa acagacattg tcaattgcaa agcaaaaatg taacacccct 781 acaatggatg ccgacaagat tgtattcaaa gtcaataatc aggtggtctc tttgaagcct 841 gagattatcg tggatcaata tgagtacaag taccctgcca tcaaagattt gaaaaagccc 901 tgtataaccc taggaaaggc tcccgattta aataaagcat acaagtcagt tttgtcaggc 961 atgagcgccg ccaaacttaa tcctgacgat gtatgttcct atttggcagc ggcaatgcag 1021 ttttttgagg ggacatgtcc ggaagactgg accagctatg gaattgtgat tgcacgaaaa 1081 ggagataaga tcaccccagg ttctctggtg gagataaaac gtactgatgt agaagggaat 1141 tgggctctga caggaggcat ggaactgaca agagacccca ctgtccctga gcatgcgtcc 1201 ttagtcggtc ttctcttgag tctgtatagg ttgagcaaaa tatccgggca aaacactggt 1261 aactataaga caaacattgc agacaggata gagcagattt ttgagacagc cccttttgtt 1321 aaaatcgtgg aacaccatac tctaatgaca actcacaaaa tgtgtgctaa ttggagtact 1381 ataccaaact tcagattttt ggccggaacc tatgacatgt ttttctcccg gattgagcat 1441 ctatattcag caatcagagt gggcacagtt gtcactgctt atgaagactg ttcaggactg 1501 gtatcattta ctgggttcat aaaacaaatc aatctcaccg ctagagaggc aatactatat 1561 ttcttccaca agaactttga ggaagagata agaagaatgt ttgagccagg gcaggagaca 1621 gctgttcctc actcttattt catccacttc cgttcactag gcttgagtgg gaaatctcct 1681 tattcatcaa atgctgttgg tcacgtgttc aatctcattc actttgtagg atgctatatg 1741 ggtcaagtca gatccctaaa tgcaacggtt attgctgcat gtgctcctca tgaaatgtct 1801 gttctagggg gctatctggg agaggaattc ttcgggaaag ggacatttga aagaagattc 1861 ttcagagatg agaaagaact tcaagaatac gaggcggctg aactgacaaa gactgacgta 1921 gcactggcag atgatggaac tgtcaactct gacgacgagg actacttttc aggtgaaacc 1981 agaagtccgg aggctgttta tactcgaatc atgatgaatg gaggtcgact aaagagatct 2041 cacatacgga gatatgtctc agtcagttcc aatcatcaag cccgtccaaa ctcattcgcc 2101 gagtttctaa acaagacata ttcgagtgac tcataagaag ttgaataaca aaatgccgga 2161 aatctacgga ttgtgtatat ccatcatgaa aaaaactaac acccctcctt tcgaaccatc 2221 ccaaacatga gcaagatctt tgtcaatcct agtgctatta gagccggtct ggccgatctt 2281 gagatggctg aagaaactgt tgatctgatc aatagaaata tcgaagacaa tcaggctcat 2341 ctccaagggg aacccataga ggtggacaat ctccctgagg atatggggcg acttcacctg 2401 gatgatggaa aatcgcccaa ccatggtgag atagccaagg tgggagaagg caagtatcga 2461 gaggactttc agatggatga aggagaggat cctagcttcc tgttccagtc atacctggaa 2521 aatgttggag tccaaatagt cagacaaatg aggtcaggag agagatttct caagatatgg 2581 tcacagaccg tagaagagat tatatcctat gtcgcggtca actttcccaa ccctccagga 2641 aagtcttcag aggataaatc aacccagact actggccgag agctcaagaa ggagacaaca 2701 cccactcctt ctcagagaga aagccaatca tcgaaagcca ggatggcggc tcaaattgct 2761 tctggccctc cagcccttga atggtcggct accaatgaag aggatgatct atcagtggag 2821 gctgagatcg ctcaccagat tgcagaaagt ttctccaaaa aatataagtt tccctctcga 2881 tcctcaggga tactcttgta taattttgag caattgaaaa tgaaccttga tgatatagtt 2941 aaagaggcaa aaaatgtacc aggtgtgacc cgtttagccc atgacgggtc caaactcccc 3001 ctaagatgtg tactgggatg ggtcgctttg gccaactcta agaaattcca gttgttagtc 3061 gaatccgaca agctgagtaa aatcatgcaa gatgacttga atcgctatac atcttgctaa 3121 ccgaacctct cccctcagtc cctctagaca ataaaatccg agatgtccca aagtcaacat 3181 gaaaaaaaca ggcaacacca ctgataaatc gatgaacctc ctacgtaaga tagtgaaaaa 3241 ccgcagggac gaggacactc aaaaatcctc tcccgcgtca gcccctctgg atgacgatga 3301 cttgtggctt ccaccccctg aatacgtccc gctgaaagaa cttacaggca agaagaacat 3361 gaggaacttt tgtatcaacg gaagggttaa agtgtgtagc ccgaatggtt actcgttcag 3421 gatcctgcgg cacattctga aatcattcga cgagatatat tctgggaatc ataggatgat 3481 cgggttagtc aaagtggtta ttggactggc tttgtcagga tctccagtcc ctgagggcct 3541 gaactgggta tacaaattga ggagaacctt tatcttccag tgggctgatt ccaggggccc 3601 tcttgaaggg gaggagttgg aatactctca ggagatcact tgggatgatg atactgagtt 3661 cgtcggattg caaataagag tgattgcaaa acagtgtcat atccagggca gagtctggtg 3721 tatcaacatg aacccgagag catgtcaact atggtctgac atgtctcttc agacacaaag 3781 gtccgaagag gacaaagatt cctctctgct tctagaataa cgtacgtcag attatatccc 3841 gcaaatttat cacttgttta cctctggagg agagaacata tgggctcaac tccaaccctt 3901 gggagcaata taacaaaaaa catgttatgg tgccattaaa ccgctgcatt tcatcaaagt 3961 caagttgatt acctttacat tttgatcctc ttggatgtga aaaaaactat taacatccct 4021 caaaggacct gcaggatcca tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg 4081 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 4141 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 4201 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg 4261 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 4321 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 4381 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 4441 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 4501 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 4561 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 4621 cggccccgtg ctgctgccca acaaccacta cctgagcacc cagtccgccc tgagcaaaga 4681 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 4741 tctcggcatg gacgagctgt acaagtaaag cggccgaccc gcggtagctt ttcagtcgag 4801 aaaaaaacat tagatcagaa gaacaactgg caacacttct caacctgaga cttacttcaa 4861 gatgctcgat cctggagagg tctatgatga ccctattgac ccaatcgagt tagaggctga 4921 acccagagga acccccattg tccccaacat cttgaggaac tctgactaca atctcaactc 4981 tcctttgata gaagatcctg ctagactaat gttagaatgg ttaaaaacag ggaatagacc 5041 ttatcggatg actctaacag acaattgctc caggtctttc agagttttga aagattattt 5101 caagaaggta gatttgggtt ctctcaaggt gggcggaatg gctgcacagt caatgatttc 5161 tctctggtta tatggtgccc actctgaatc caacaggagc cggagatgta taacagactt 5221 ggcccatttc tattccaagt cgtcccccat agagaagctg ttgaatctca cgctaggaaa 5281 tagagggctg agaatccccc cagagggagt gttaagttgc cttgagaggg ttgattatga 5341 taatgcattt ggaaggtatc ttgccaacac gtattcctct tacttgttct tccatgtaat 5401 caccttatac atgaacgccc tagactggga tgaagaaaag accatcctag cattatggaa 5461 agatttaacc tcagtggaca tcgggaagga cttggtaaag ttcaaagacc aaatatgggg 5521 actgctgatc gtgacaaagg actttgttta ctcccaaagt tccaattgtc tttttgacag 5581 aaactacaca cttatgctaa aagatctttt cttgtctcgc ttcaactcct taatggtctt 5641 gctctctccc ccagagcccc gatactcaga tgacttgata tctcaactat gccagctgta 5701 cattgctggg gatcaagtct tgtctatgtg tggaaactcc ggctatgaag tcatcaaaat 5761 attggagcca tatgtcgtga atagtttagt ccagagagca gaaaagttta ggcctctcat 5821 tcattccttg ggagactttc ctgtatttat aaaagacaag gtaagtcaac ttgaagagac 5881 gttcggtccc tgtgcaagaa ggttctttag ggctctggat caattcgaca acatacatga 5941 cttggttttt gtgtttggct gttacaggca ttgggggcac ccatatatag attatcgaaa 6001 gggtctgtca aaactatatg atcaggttca ccttaaaaaa atgatagata agtcctacca 6061 ggagtgctta gcaagcgacc tagccaggag gatccttaga tggggttttg ataagtactc 6121 caagtggtat ctggattcaa gattcctagc ccgagaccac cccttgactc cttatatcaa 6181 aacccaaaca tggccaccca aacatattgt agacttggtg ggggatacat ggcacaagct 6241 cccgatcacg cagatctttg agattcctga atcaatggat ccgtcagaaa tattggatga 6301 caaatcacat tctttcacca gaacgagact agcttcttgg ctgtcagaaa accgaggggg 6361 gcctgttcct agcgaaaaag ttattatcac ggccctgtct aagccgcctg tcaatccccg 6421 agagtttctg aggtctatag acctcggagg attgccagat gaagacttga taattggcct 6481 caagccaaag gaacgggaat tgaagattga aggtcgattc tttgctctaa tgtcatggaa 6541 tctaagattg tattttgtca tcactgaaaa actcttggcc aactacatct tgccactttt 6601 tgacgcgctg actatgacag acaacctgaa caaggtgttt aaaaagctga tcgacagggt 6661 caccgggcaa gggcttttgg actattcaag ggtcacatat gcatttcacc tggactatga 6721 aaagtggaac aaccatcaaa gattagagtc aacagaggat gtattttctg tcctagatca 6781 agtgtttgga ttgaagagag tgttttctag aacacacgag ttttttcaaa aggcctggat 6841 ctattattca gacagatcag acctcatcgg gttacgggag gatcaaatat actgcttaga 6901 tgcgtccaac ggcccaacct gttggaatgg ccaggatggc gggctagaag gcttacggca 6961 gaagggctgg agtctagtca gcttattgat gatagataga gaatctcaaa tcaggaacac 7021 aagaaccaaa atactagctc aaggagacaa ccaggtttta tgtccgacat acatgttgtc 7081 gccagggcta tctcaagagg ggctcctcta tgaattggag agaatatcaa ggaatgcact 7141 ttcgatatac agagccgtcg aggaaggggc atctaagcta gggctgatca tcaagaaaga 7201 agagaccatg tgtagttatg acttcctcat ctatggaaaa acccctttgt ttagaggtaa 7261 catattggtg cctgagtcca aaagatgggc cagagtctct tgcgtctcta atgaccaaat 7321 agtcaacctc gccaatataa tgtcgacagt gtccaccaat gcgctaacag tggcacaaca 7381 ctctcaatct ttgatcaaac cgatgaggga ttttctgctc atgtcagtac aggcagtctt 7441 tcactacctg ctatttagcc caatcttaaa gggaagagtt tacaagattc tgagcgctga 7501 aggggagagc tttctcctag ccatgtcaag gataatctat ctagatcctt ctttgggagg 7561 gatatctgga atgtccctcg gaagattcca tatacgacag ttctcagacc ctgtctctga 7621 agggttatcc ttctggagag agatctggtt aagctcccaa gagtcctgga ttcacgcgtt 7681 gtgtcaagag gctggaaacc cagatcttgg agagagaaca ctcgagagct tcactcgcct 7741 tctagaagat ccgaccacct taaatatcag aggaggggcc agtcctacca ttctactcaa 7801 ggatgcaatc agaaaggctt tatatgacga ggtggacaag gtggaaaatt cagagtttcg 7861 agaggcaatc ctgttgtcca agacccatag agataatttt atactcttct taatatctgt 7921 tgagcctctg tttcctcgat ttctcagtga gctattcagt tcgtcttttt tgggaatccc 7981 cgagtcaatc attggattga tacaaaactc ccgaacgata agaaggcagt ttagaaagag 8041 tctctcaaaa actttagaag aatccttcta caactcagag atccacggga ttagtcggat 8101 gacccagaca cctcagaggg ttgggggggt gtggccttgc tcttcagaga gggcagatct 8161 acttagggag atctcttggg gaagaaaagt ggtaggcacg acagttcctc acccttctga 8221 gatgttggga ttacttccca agtcctctat ttcttgcact tgtggagcaa caggaggagg 8281 caatcctaga gtttctgtat cagtactccc gtcctttgat cagtcatttt tttcacgagg 8341 ccccctaaag ggatacttgg gctcgtccac ctctatgtcg acccagctat tccatgcatg 8401 ggaaaaagtc actaatgttc atgtggtgaa gagagctcta tcgttaaaag aatctataaa 8461 ctggttcatt actagagatt ccaacttggc tcaagctcta attaggaaca ttatgtctct 8521 gacaggccct gatttccctc tagaggaggc ccctgtcttc aaaaggacgg ggtcagcctt 8581 gcataggttc aagtctgcca gatacagcga aggagggtat tcttctgtct gcccgaacct 8641 cctctctcat atttctgtta gtacagacac catgtctgat ttgacccaag acgggaagaa 8701 ctacgatttc atgttccagc cattgatgct ttatgcacag acatggacat cagagctggt 8761 acagagagac acaaggctaa gagactctac gtttcattgg cacctccgat gcaacaggtg 8821 tgtgagaccc attgacgacg tgaccctgga gacctctcag atcttcgagt ttccggatgt 8881 gtcgaaaaga atatccagaa tggtttctgg ggctgtgcct cacttccaga ggcttcccga 8941 tatccgtctg agaccaggag attttgaatc tctaagcggt agagaaaagt ctcaccatat 9001 cggatcagct caggggctct tatactcaat cttagtggca attcacgact caggatacaa 9061 tgatggaacc atcttccctg tcaacatata cggcaaggtt tcccctagag actatttgag 9121 agggctcgca aggggagtat tgataggatc ctcgatttgc ttcttgacaa gaatgacaaa 9181 tatcaatatt aatagacctc ttgaattggt ctcaggggta atctcatata ttctcctgag 9241 gctagataac catccctcct tgtacataat gctcagagaa ccgtctctta gaggagagat 9301 attttctatc cctcagaaaa tccccgccgc ttatccaacc actatgaaag aaggcaacag 9361 atcaatcttg tgttatctcc aacatgtgct acgctatgag cgagagataa tcacggcgtc 9421 tccagagaat gactggctat ggatcttttc agactttaga agtgccaaaa tgacgtacct 9481 atccctcatt acttaccagt ctcatcttct actccagagg gttgagagaa acctatctaa 9541 gagtatgaga gataacctgc gacaattgag ttctttgatg aggcaggtgc tgggcgggca 9601 cggagaagat accttagagt cagacgacaa cattcaacga ctgctaaaag actctttacg 9661 aaggacaaga tgggtggatc aagaggtgcg ccatgcagct agaaccatga ctggagatta 9721 cagccccaac aagaaggtgt cccgtaaggt aggatgttca gaatgggtct gctctgctca 9781 acaggttgca gtctctacct cagcaaaccc ggcccctgtc tcggagcttg acataagggc 9841 cctctctaag aggttccaga accctttgat ctcgggcttg agagtggttc agtgggcaac 9901 cggtgctcat tataagctta agcctattct agatgatctc aatgttttcc catctctctg 9961 ccttgtagtt ggggacgggt caggggggat atcaagggca gtcctcaaca tgtttccaga 10021 tgccaagctt gtgttcaaca gtcttttaga ggtgaatgac ctgatggctt ccggaacaca 10081 tccactgcct ccttcagcaa tcatgagggg aggaaatgat atcgtctcca gagtgataga 10141 tcttgactca atctgggaaa aaccgtccga cttgagaaac ttggcaacct ggaaatactt 10201 ccagtcagtc caaaagcagg tcaacatgtc ctatgacctc attatttgcg atgcagaagt 10261 tactgacatt gcatctatca accggatcac cctgttaatg tccgattttg cattgtctat 10321 agatggacca ctctatttgg tcttcaaaac ttatgggact atgctagtaa atccaaacta 10381 caaggctatt caacacctgt caagagcgtt cccctcggtc acagggttta tcacccaagt 10441 aacttcgtct ttttcatctg agctctacct ccgattctcc aaacgaggga agtttttcag 10501 agatgctgag tacttgacct cttccaccct tcgagaaatg agccttgtgt tattcaattg 10561 tagcagcccc aagagtgaga tgcagagagc tcgttccttg aactatcagg atcttgtgag 10621 aggatttcct gaagaaatca tatcaaatcc ttacaatgag atgatcataa ctctgattga 10681 cagtgatgta gaatcttttc tagtccacaa gatggttgat gatcttgagt tacagagggg 10741 aactctgtct aaagtggcta tcattatagc catcatgata gttttctcca acagagtctt 10801 caacgtttcc aaacccctaa ctgacccctc gttctatcca ccgtctgatc ccaaaatcct 10861 gaggcacttc aacatatgtt gcagtactat gatgtatcta tctactgctt taggtgacgt 10921 ccctagcttc gcaagacttc acgacctgta taacagacct ataacttatt acttcagaaa 10981 gcaagtcatt cgagggaacg tttatctatc ttggagttgg tccaacgaca cctcagtgtt 11041 caaaagggta gcctgtaatt ctagcctgag tctgtcatct cactggatca ggttgattta 11101 caagatagtg aagactacca gactcgttgg cagcatcaag gatctatcca gagaagtgga 11161 aagacacctt cataggtaca acaggtggat caccctagag gatatcagat ctagatcatc 11221 cctactagac tacagttgcc tgtgaaccgg atactcctgg aagcctgccc atgctaagac 11281 tcttgtgtga tgtatcttga aaaaaacaag atcctaaatc tgaacctttg gttgtttgat 11341 tgtttttctc atttttgttg tttatttgtt aagcgtgggt cggcatggca tctccacctc 11401 ctcgcggtcc gacctgggca tccgaaggag gacgcacgtc cactcggatg gctaagggag 11461 aagggcgaat tccagcacac tggcggccgt tactagtgga tccgagctcg gtaccgagct 11521 cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc 11581 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 11641 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 11701 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 11761 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 11821 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 11881 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 11941 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 12001 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 12061 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 12121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 12181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 12241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 12301 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 12361 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 12421 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 12481 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 12541 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 12601 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 12661 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta 12721 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc 12781 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt 12841 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt 12901 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac 12961 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt 13021 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg 13081 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga 13141 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg 13201 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 13261 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 13321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 13381 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 13441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 13501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 13561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 13621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 13681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 13741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 13801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 13861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 13921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 13981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 14041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 14101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 14161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 14221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 14281 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 14341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 14401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 14461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 14521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 14581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 14641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 14701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 14761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 14821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 14881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 14941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 15001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 15061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 15121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 15181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 15241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 15301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 15361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 15421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 15481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 15541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 15601 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc 15661 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag 15721 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag 15781 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt 15841 t //