LOCUS       Exported                 772 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    pLX304
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26. ():.
  PUBMED    21706014
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(87..211)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             213..254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(349..369)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
       61 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
      121 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      181 tttctcgttc agctttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
      241 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
      301 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
      361 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
      421 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
      481 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
      541 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
      601 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
      661 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
      721 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg cc
//
