LOCUS Exported 7692 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBp-FGFR2c-WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7692) AUTHORS Liao RG, Jung J, Tchaicha JH, Wilkerson MD, Sivachenko A, Beauchamp EM, Liu Q, Pugh TJ, Pedamallu CS, Hayes DN, Gray NS, Getz G, Wong KK, Haddad RI, Meyerson M, Hammerman PS TITLE Inhibitor-sensitive FGFR2 and FGFR3 mutations in lung squamous cell carcinoma. JOURNAL Cancer Res. 2013 Jun 20. PUBMED 23786770 REFERENCE 2 (bases 1 to 7692) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7692 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 8..477 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 542..741 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 934..1350 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1318..1334 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" protein_bind 1378..1402 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind 1801..1812 /label=ZF binding site /bound_moiety="CCR5TC zinc-finger domain" /note="target sequence for the CCR5TC zinc-finger domain from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 2008; Gross et al., 2013)" protein_bind complement(3872..3896) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(3932..3952) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3937..4266 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4117..4252 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4179..4198 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4276..4875 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind complement(4276..4295) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4772..4792 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 4974..5567 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(5768..5785) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5939..6526) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6019..6038) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6704..7564) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7327..7346 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7565..7669) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacatcaca 1381 agtttgtaca aaaaagcagg caccatggtc agctggggtc gtttcatctg cctggtcgtg 1441 gtcaccatgg caaccttgtc cctggcccgg ccctccttca gtttagttga ggataccaca 1501 ttagagccag aagagccacc aaccaaatac caaatctctc aaccagaagt gtacgtggct 1561 gcgccagggg agtcgctaga ggtgcgctgc ctgttgaaag atgccgccgt gatcagttgg 1621 actaaggatg gggtgcactt ggggcccaac aataggacag tgcttattgg ggagtacttg 1681 cagataaagg gcgccacgcc tagagactcc ggcctctatg cttgtactgc cagtaggact 1741 gtagacagtg aaacttggta cttcatggtg aatgtcacag atgccatctc atccggagat 1801 gatgaggatg acaccgatgg tgcggaagat tttgtcagtg agaacagtaa caacaagaga 1861 gcaccatact ggaccaacac agaaaagatg gaaaagcggc tccatgctgt gcctgcggcc 1921 aacactgtca agtttcgctg cccagccggg gggaacccaa tgccaaccat gcggtggctg 1981 aaaaacggga aggagtttaa gcaggagcat cgcattggag gctacaaggt acgaaaccag 2041 cactggagcc tcattatgga aagtgtggtc ccatctgaca agggaaatta tacctgtgtg 2101 gtggagaatg aatacgggtc catcaatcac acgtaccacc tggatgttgt ggagcgatcg 2161 cctcaccggc ccatcctcca agccggactg ccggcaaatg cctccacagt ggtcggagga 2221 gacgtagagt ttgtctgcaa ggtttacagt gatgcccagc cccacatcca gtggatcaag 2281 cacgtggaaa agaacggcag taaatacggg cccgacgggc tgccctacct caaggttctc 2341 aaggccgccg gtgttaacac cacggacaaa gagattgagg ttctctatat tcggaatgta 2401 acttttgagg acgctgggga atatacgtgc ttggcgggta attctattgg gatatccttt 2461 cactctgcat ggttgacagt tctgccagcg cctggaagag aaaaggagat tacagcttcc 2521 ccagactacc tggagatagc catttactgc ataggggtct tcttaatcgc ctgtatggtg 2581 gtaacagtca tcctgtgccg aatgaagaac acgaccaaga agccagactt cagcagccag 2641 ccggctgtgc acaagctgac caaacgtatc cccctgcgga gacaggtaac agtttcggct 2701 gagtccagct cctccatgaa ctccaacacc ccgctggtga ggataacaac acgcctctct 2761 tcaacggcag acacccccat gctggcaggg gtctccgagt atgaacttcc agaggaccca 2821 aaatgggagt ttccaagaga taagctgaca ctgggcaagc ccctgggaga aggttgcttt 2881 gggcaagtgg tcatggcgga agcagtggga attgacaaag acaagcccaa ggaggcggtc 2941 accgtggccg tgaagatgtt gaaagatgat gccacagaga aagacctttc tgatctggtg 3001 tcagagatgg agatgatgaa gatgattggg aaacacaaga atatcataaa tcttcttgga 3061 gcctgcacac aggatgggcc tctctatgtc atagttgagt atgcctctaa aggcaacctc 3121 cgagaatacc tccgagcccg gaggccaccc gggatggagt actcctatga cattaaccgt 3181 gttcctgagg agcagatgac cttcaaggac ttggtgtcat gcacctacca gctggccaga 3241 ggcatggagt acttggcttc ccaaaaatgt attcatcgag atttagcagc cagaaatgtt 3301 ttggtaacag aaaacaatgt gatgaaaata gcagactttg gactcgccag agatatcaac 3361 aatatagact attacaaaaa gaccaccaat gggcggcttc cagtcaagtg gatggctcca 3421 gaagccctgt ttgatagagt atacactcat cagagtgatg tctggtcctt cggggtgtta 3481 atgtgggaga tcttcacttt agggggctcg ccctacccag ggattcccgt ggaggaactt 3541 tttaagctgc tgaaggaagg acacagaatg gataagccag ccaactgcac caacgaactg 3601 tacatgatga tgagggactg ttggcatgca gtgccctccc agagaccaac gttcaagcag 3661 ttggtagaag acttggatcg aattctcact ctcacaacca atgaggaata cttggacctc 3721 agccaacctc tcgaacagta ttcacctagt taccctgaca caagaagttc ttgttcttca 3781 ggagatgatt ctgttttttc tccagacccc atgccttacg aaccatgcct tcctcagtat 3841 ccacacataa acggcagtgt taaaacatag aacccagctt tcttgtacaa agtggtgatg 3901 taggaattcg ccagcacagt ggtcgacctg tggaatgtgt gtcagttagg gtgtggaaag 3961 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 4021 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4081 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 4141 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 4201 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 4261 tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4321 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4381 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4441 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4501 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 4561 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 4621 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 4681 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 4741 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 4801 ttcaccgtca ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc 4861 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga 4921 ccccatgcat cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa 4981 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa 5041 aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa 5101 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca 5161 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc 5221 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac 5281 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta 5341 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag 5401 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc 5461 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga 5521 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttcc gacttgtggt 5581 ctcgctgcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtcttcac 5641 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca 5701 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggcgctctt 5761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 5821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 5881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 5941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 6061 ctcctgttcc gaccctgccg cttaccggat accgtccgcc tttctccctt cgggaagcgt 6121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 6301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 6361 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 6421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 6481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 6541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 6601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gtttgcggcc 6661 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 6721 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 6781 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 6841 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 6901 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 6961 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 7021 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7081 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7141 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 7201 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 7261 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 7321 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 7381 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 7441 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 7501 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 7561 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 7621 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 7681 gaaaagtgcc ac //