LOCUS Exported 635 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.. ACCESSION . VERSION . KEYWORDS pLKO.1-blast SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 635) AUTHORS Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, Martin-Belmonte F, Mostov KE TITLE A molecular network for de novo generation of the apical surface and lumen. JOURNAL Nat Cell Biol. 2010 Oct 3. ():. PUBMED 20890297 REFERENCE 2 (bases 1 to 635) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..635 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 114..354 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 114..134 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 285..304 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_feature 425..542 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg 61 gatctcgacg gtatcgatca cgagactagc ctcgagcggc cgcccccttc accgagggcc 121 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg 181 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat 241 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta 301 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca 361 ccggtccgca ggtatgcacg cgtgaattct cgacctcgag acaaatggca gtattcatcc 421 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca 481 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt 541 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg 601 ttgcgccttt tccaaggcag ccctgggttt gcgca //