LOCUS Exported 3997 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides the CDS of the Renilla protein as a level 0 GoldenBraid part. ACCESSION . VERSION . KEYWORDS pRenilla (GB0056) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3997) AUTHORS Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. JOURNAL Plant Physiol. 2013 Jul;162(3):1618-31. PUBMED 23669743 REFERENCE 2 (bases 1 to 3997) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3997 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(12..947) /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" promoter complement(1047..1065) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(1048..1065) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(1083..1099) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1083..1099) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1096..1118) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1107..1123 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1131..1161) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1176..1197 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1314..1331) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1485..2073) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1565..1584) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2244..3104) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2867..2886 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3105..3209) /gene="bla" /label=AmpR promoter rep_origin complement(3287..3742) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3424..3445) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3636..3655 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3868..3890 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3882..3899 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3883..3899 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3906..3925 /label=T7 /note="T7 promoter, forward primer" promoter 3906..3924 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 3955..3964 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3959..3968 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ctcgaagctc attattgttc atttttgaga actcgctcaa cgaacgattt gatatatttt 61 cccatttcat caggtgcatc ttcttgcgaa aaatgaagac cttttacttt gacaaattca 121 gtattaggaa acttcttggc accttcaaca atagcattgg aaaagaatcc tgggtccgat 181 tcaataaaca tttttggtaa atcatcactt gcacgtagat aagcattata attcctaaca 241 atttgtacaa cgtcaggttt accacctttt actaacggga tttcacgagg ccatgataat 301 gttggacgac gaacttcacc tttctctttg aatggttcaa gatatgctgc aaattcttct 361 ggttctaact ttctcatgat ttttgatggc aacatggttt ccacgaagaa gttattctcc 421 aaaaccattt tttctccttc ttcagatttg atcaacgcaa tatcttcttc aatatcaggc 481 cattcatccc atgattcaat cacatctact acactttcag cgtgaactat tgctttgatc 541 ttatcttgat gctcatagct ataatgaaat gccaaacaag caccccaatc atggccgaca 601 aaaatgatct tctttggtaa attaagaagt tcaaaccatg cagtaagata tttgtaatga 661 tcaagtaacc tataagaacc attaccagat ttgcctgatt tgcccatacc aataaggtct 721 ggtataatac accgcgctac tggctcaata tgtggcacaa catgtcgcca taaataagaa 781 gaggccgcgt taccatgtaa aaaaataaca gcattttctg catgtttttc tgaatcataa 841 taattaataa atgaatcaag aacattcatt tgtttacatc tggcccacca ctgcggacca 901 gttatcatcc gtttcctttg ttctggatca taaactttcg aagtcattcg agaccactca 961 tcgcccaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca 1021 acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca 1081 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 1141 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 1201 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 1261 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 1321 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 1381 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 1441 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 1501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 1561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 1621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 1681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 1741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 1801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 1861 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 1921 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 1981 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 2041 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 2101 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 2161 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 2221 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 2281 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 2341 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg 2401 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 2461 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 2521 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 2581 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 2641 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 2701 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 2761 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 2821 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 2881 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 2941 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 3001 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 3061 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 3121 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 3181 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg 3241 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt 3301 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 3361 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 3421 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 3481 aaaaccgtct atcagggcgc tggcccacta cgtgaaccat caccctaatc aagttttttg 3541 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 3601 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 3661 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 3721 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc 3781 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 3841 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 3901 aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc 3961 atggcggccg cgggaattcg attgggcgat gagtggt //