LOCUS       Exported                1049 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Expresses OsTIR1 under the control of the Tet promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pMK243 (Tet-OsTIR1-PURO)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..377
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    54..72
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    90..108
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    126..144
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    162..180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    198..216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ttctctctcg agtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat
       61 cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag
      121 tttactccct atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga
      181 acgtatctac agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat
      241 cagtgataga gaacgtataa gctttgctta tgtaaaccag ggcgcctata aaagagtgct
      301 gattttttga gtaaacttca attccacaac acttttgtct tataccaact ttccgtacca
      361 cttcctaccc tcgtaaagaa ttcgcggccg cacgcgtgcc accatgacat actttcctga
      421 agaggtcgtc gaacacattt ttagcttcct gcctgcacag agagatagaa acacagtgag
      481 cctggtctgc aaagtgtggt acgagatcga acgcctgagc cggagaggag tgttcgtcgg
      541 caactgctat gctgtgagag caggcagggt cgccgctagg tttccaaatg tgcgcgcact
      601 gaccgtcaag gggaaacccc acttcgccga ctttaacctg gtgccccctg attggggagg
      661 atacgccggc ccttggatcg aggcagccgc tcgcggctgt catggactgg aggaactgcg
      721 catgaagcga atggtggtct ctgacgaaag tctggagctg ctggctcgga gcttccctag
      781 gtttcgcgca ctggtgctga tttcttgcga aggcttcagc accgatggac tggcagccgt
      841 ggcctcccac tgtaagctgc tgcgggagct ggacctccag gagaatgaag tggaggatag
      901 aggccccaga tggctgtctt gcttcccaga ctcatgtacc agcctggtgt ccctgaactt
      961 tgcctgcatc aaaggcgaag tgaatgctgg gtccctggag cggctggtct caagaagccc
     1021 caacctgagg tctctgcggc tgaaccgga
//
