LOCUS Exported 1005 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expresses OsTIR1 under the control of the Tet promoter. ACCESSION . VERSION . KEYWORDS pMK243 (Tet-OsTIR1-PURO) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1005) AUTHORS Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT TITLE Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. JOURNAL Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. PUBMED 27052166 REFERENCE 2 (bases 1 to 1005) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1005 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(172..918) /codon_start=1 /product="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" /label=Tet-On(R) 3G /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" ORIGIN 1 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 61 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 121 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctggaga tttacccggg 181 gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc 241 gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggtcggccc 301 gccgctttcg cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag 361 gaaggcaggt tcggctccct gccggtcgaa cagctcaatt gcttgtttca gaagtggggg 421 catagaatcg gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc 481 caatacgcag cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag 541 ggagaagcct tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc 601 tgttgggcgg gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg 661 gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagga 721 gtgggtatga tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt 781 cttcacgtgc cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt 841 cgtcaggcct tcgataccga ctccattgag taattccaga gcagagttta tgactttgct 901 cttgtccagt ctagacatgg taattcgatg atccccctgg ggagagaggt cggtgattcg 961 gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc ttgca //