LOCUS Exported 1018 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION C-terminal tagging with mCherry2 using CRIPSR/Cas9. ACCESSION . VERSION . KEYWORDS pMK280 (mCherry2-NeoR) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1018) AUTHORS Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT TITLE Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. JOURNAL Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. PUBMED 27052166 REFERENCE 2 (bases 1 to 1018) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1018 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 7..26 /label=T7 /note="T7 promoter, forward primer" promoter 7..25 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 64..774 /codon_start=1 /product="derivative of the monomeric red fluorescent protein mCherry" /label=mCherry2 /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(209..227) /label=mCherry-R /note="mCherry, reverse primer" polyA_signal 892..1013 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(929..948) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 983..1002 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 cgcgcgtaat acgactcact atagggcgaa ttggagctcg gatccggtgc aggcgccgct 61 agcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc 121 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag 181 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg 241 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag 301 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caattgggag 361 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag 421 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc 481 gtaatgcagt gtcgtaccat gggctgggag gcctccactg agcggatgta ccccgaggac 541 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac 601 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac 661 gtcgacatca agttggacat cctttcccac aacgaggact acaccatcgt ggaacagtac 721 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaatctaga 781 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 841 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 901 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 961 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcg //