LOCUS       Exported                 532 bp ds-DNA     linear   SYN 10-JUL-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-Wnt3-V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctc
      121 cgcggccgcc cccttcacca tggagcccca cctgctcggg ctgctcctcg gcctcctgct
      181 cggtggcacc agggtcctcg ctggctaccc aatttggtgg tccctggccc tgggccagca
      241 gtacacatct ctgggctcac agcccctgct ctgcggctcc atcccaggcc tggtccccaa
      301 gcaactgcgc ttctgccgca attacatcga gatcatgccc agcgtggccg agggcgtgaa
      361 gctgggcatc caggagtgcc agcaccagtt ccggggccgc cgctggaact gcaccaccat
      421 agatgacagc ctggccatct ttgggcccgt cctcgacaaa gccacccgcg agtcggcctt
      481 cgttcacgcc atcgcctcgg ccggcgtggc cttcgccgtc acccgctcct gc
//
