LOCUS       Exported                 840 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 
            promoter, and tet-incucibile RPR1 promoter with NotI cloning site 
            adjacent to gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Smith JD, Suresh S, Schlecht U, Wu M, Wagih O, Peltz G, Davis RW, 
            Steinmetz LM, Parts L, St Onge RP
  TITLE     Quantitative CRISPR interference screens in yeast identify 
            chemical-genetic interactions and new rules for guide RNA design.
  JOURNAL   Genome Biol. 2016 Mar 8;17(1):45. doi: 10.1186/s13059-016-0900-9.
  PUBMED    26956608
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    448..466
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        539..614
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      627..689
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctggc cttttgctcg
      121 ggggatctgc caattgaaca taacatggta gttacatata ctagtaatat ggttcggcac
      181 acattaaaag tataaaaact atctgaatta cgaattacat atattggtca taaaaatcaa
      241 tcaatcatcg tgtgttttat atgtctctta tctaagtata agaatatcca tagttaatat
      301 tcacttacgc taccttttaa cctgtaatca ttgtcaacag gatatgttaa cgacccacat
      361 tgataaacgc tagtatttct ttttcctctt cttattggcc ggctgtctct atactcccct
      421 atagtctgtt tcttttcgtt tcgattgtcc ctatcagtga tagagatggc gcacatggta
      481 cgctgtggtg ctcgcggctg ggaacgaaac tctgggagct gcgattggca gcggccgcgt
      541 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      601 caccgagtcg gtgctttttt ctcgagccat atccaacttc caatttaatc tttctttttt
      661 aattttcact tatttgcgat acagaaagaa aaaagcgata gtaactattg aattttgttt
      721 ggatttggtt agattagata tggtttctct ttatatttac atgctaaaaa tgggctacac
      781 cagagataca taattagata tatatacgcc agtacacctt atcggcccaa gccttgtccc
//
