LOCUS       Exported                 769 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  J8AAV-HDR U6sgCtnnb1.1 template.
ACCESSION   .
VERSION     .
KEYWORDS    J8AAV-HDR Ctnnb1.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Yin H, Song CQ, Dorkin JR, Zhu LJ, Li Y, Wu Q, Park A, Yang J, 
            Suresh S, Bizhanova A, Gupta A, Bolukbasi MF, Walsh S, Bogorad RL, 
            Gao G, Weng Z, Dong Y, Koteliansky V, Wolfe SA, Langer R, Xue W, 
            Anderson DG
  TITLE     Therapeutic genome editing by combined viral and non-viral delivery 
            of CRISPR system components in vivo.
  JOURNAL   Nat Biotechnol. 2016 Feb 1. doi: 10.1038/nbt.3471.
  PUBMED    26829318
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        224..299
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccgctgtggt ggtggcacca gaagttttag agctagaaat
      241 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      301 tttttgtttt agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg
      361 cgccaattct gcagacaaat ggctttccct agagagggga cattctctag aagcaccgta
      421 tgcctacaat ctgtttctac tgagaagagc ttcctgacac cgtggctgct gtgtattttt
      481 agtgtatgcc atggtgaact ggcttttggt gtcggggcac atagcctagt ataggtagca
      541 gaatcacggt gacctgggtt aaaaacaaac ttaagtttta atgacttgat ggaatttttc
      601 agggtacctg aagctcagcg cacagctgct gtgacaccgc tgcgtggaca atggctactc
      661 aaggtttgtg attcagatgc ctgtctgagg atctgcctca tagccctgct gcctggccag
      721 actgcctttg ttctcttccc ttctgcacac taccactggc ttggtgaaa
//
