LOCUS       Exported                8749 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    MigR1-AE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Yan M, Burel SA, Peterson LF, Kanbe E, Iwasaki H, Boyapati A, Hines 
            R, Akashi K, Zhang DE
  TITLE     Deletion of an AML1-ETO C-terminal NcoR/SMRT-interacting region 
            strongly induces leukemia development.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Dec 7. 101(49):17186-91.
  PUBMED    15569932
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1974..1992
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2030..2052)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2152..2171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2365..2387
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2515..2534
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2734..3250
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3314..3655
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3722..4138
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4068..4090
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4106..4122
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4173..4199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6492..7044
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6636..6653)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6863..6882
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7056..7775
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7101..7122)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7362..7381)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7709..7730
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             7877..8391
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8553..8569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8577..8607)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8622..8643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     2041 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     2101 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2161 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     2221 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2281 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     2341 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa
     2401 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     2461 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     2521 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     2581 tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct
     2641 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
     2701 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     2761 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
     2821 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
     2881 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
     2941 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     3001 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     3061 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
     3121 tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa
     3181 tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg
     3241 gtctttcatt tggaggttcc accgagattt ggagacccct gcccagggac caccgacccc
     3301 cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt
     3361 ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat
     3421 ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg
     3481 tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa
     3541 tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
     3601 tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg
     3661 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta
     3721 gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg
     3781 gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc
     3841 agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat
     3901 cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc
     3961 ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt
     4021 acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc
     4081 tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg
     4141 aattagatct ctcgaggttc tagaccatgg catacccata cgacgtgcct gactacgcct
     4201 cccgtatccc cgtagatgcc agcacgagcc gccgcttcac gccgccttcc accgcgctga
     4261 gcccaggcaa gatgagcgag gcgttgccgc tgggcgcccc ggacgccggc gctgccctgg
     4321 ccggcaagct gaggagcggc gaccgcagca tggtggaggt gctggccgac cacccgggcg
     4381 agctggtgcg caccgacagc cccaacttcc tctgctccgt gctgcctacg cactggcgct
     4441 gcaacaagac cctgcccatc gctttcaagg tggtggccct aggggatgtt ccagatggca
     4501 ctctggtcac tgtgatggct ggcaatgatg aaaactactc ggctgagctg agaaatgcta
     4561 ccgcagccat gaagaaccag gttgcaagat ttaatgacct caggtttgtc ggtcgaagtg
     4621 gaagagggaa aagcttcact ctgaccatca ctgtcttcac aaacccaccg caagtcgcca
     4681 cctaccacag agccatcaaa atcacagtgg atgggccccg agaacctcga aatcgtactg
     4741 agaagcactc cacaatgcca gactcacctg tggatgtgaa gacgcaatct aggctgactc
     4801 ctccaacaat gccacctccc ccaactactc aaggagctcc aagaaccagt tcatttacac
     4861 cgacaacgtt aactaatggc acgagccatt ctcctacagc cttgaatggc gccccctcac
     4921 cacccaatgg cttcagcaat gggccttcct cttcttcctc ctcctctctg gctaatcaac
     4981 agctgccccc agcctgtggt gccaggcaac tcagcaagct gaaaaggttc cttactaccc
     5041 tgcagcagtt tggcaatgac atttcacccg agataggaga aagagttcgc accctcgttc
     5101 tgggactagt gaactccact ttgacaattg aagaatttca ttccaaactg caagaagcta
     5161 ctaacttccc actgagacct tttgtcatcc catttttgaa ggccaacttg cccctgctgc
     5221 agcgtgagct cctccactgc gcaagactgg ccaaacagaa ccctgcccag tacctcgccc
     5281 agcatgaaca gctgcttctg gatgccagca ccacctcacc tgttgactcc tcagagctgc
     5341 ttctcgatgt gaacgaaaac gggaagaggc gaactccaga cagaaccaaa gaaaatggct
     5401 ttgacagaga gcctttgcac tcagaacatc caagcaagcg accatgcact attagcccag
     5461 gccagcggta cagtccaaat aacggcttat cctaccagcc caatggcctg cctcacccta
     5521 ccccacctcc acctcagcat taccgtttgg atgatatggc cattgcccac cactacaggg
     5581 actcctatcg acaccccagc cacagggacc tcagggacag aaacagacct atggggttgc
     5641 atggcacacg tcaagaagaa atgattgatc acagactaac agacagagaa tgggcagaag
     5701 agtggaaaca tcttgaccat ctgttaaact gcataatgga catggtagaa aaaacaaggc
     5761 gatctctcac cgtactaagg cggtgtcaag aagcagaccg ggaagaattg aattactgga
     5821 tccggcggta cagtgacgcc gaggacttaa aaaaaggtgg cggcagtagc agcagccact
     5881 ctaggcagca gagtcccgtc aacccagacc cagttgcact agacgcgcat cgggaattcc
     5941 ttcacaggcc tgcgtctgga tacgtgccag aggagatctg gaagaaagct gaggaggccg
     6001 tcaatgaggt gaagcgccag gcgatgacgg agctgcagaa ggccgtgtct gaggcggagc
     6061 ggaaagccca cgacatgatc acaacagaga gggccaagat ggagcgcacg gtcgccgagg
     6121 ccaaacggca ggcggcggag gacgcactgg cagttatcaa tcagcaggag gattcaagcg
     6181 agagttgctg gaattgtggc cgtaaagcga gtgaaacctg cagtggctgt aacacagccc
     6241 gatactgtgg ctcattttgc cagcacaaag actgggagaa gcaccatcac atctgtggac
     6301 agaccctgca ggcccagcag cagggagaca cacctgcagt cagctcctct gtcacgccca
     6361 acagcggggc tgggagcccg atggacacac caccagcagc cactccgagg tcaaccaccc
     6421 cgggaacccc ttccaccata gagacaaccc ctcgctagtg actctagaac gaattccgcc
     6481 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     6541 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     6601 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     6661 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     6721 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     6781 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     6841 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     6901 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     6961 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     7021 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
     7081 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     7141 gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     7201 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     7261 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     7321 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     7381 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     7441 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     7501 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc
     7561 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     7621 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     7681 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     7741 ggatcactct cggcatggac gagctgtaca agtaatgaat taattaagaa ttatcaagct
     7801 tatcgatacc gtcgacctgc agccaagctt atcgataaaa taaaagattt tatttagtct
     7861 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     7921 gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta
     7981 ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     8041 ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag
     8101 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     8161 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     8221 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg
     8281 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     8341 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag
     8401 tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac
     8461 tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga
     8521 cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat tccacacaac
     8581 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgct
//
