LOCUS Exported 7535 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.. ACCESSION . VERSION . KEYWORDS Sema6b(L)-Fc-His SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7535) AUTHORS Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray TA, Kay JN, Wojtowicz WM TITLE An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. JOURNAL Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149. PUBMED 26633812 REFERENCE 2 (bases 1 to 7535) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7535 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 105..408 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 409..612 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 563..583 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 609..633 /label=LNCX /note="Human CMV promoter, forward primer" intron 748..1573 /label=CMV intron A /note="human cytomegalovirus intron A (Chapman et al., 1991)" CDS 4115..4132 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" polyA_signal 4158..4279 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4195..4214) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4249..4268 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4317..4334) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4488..5076) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4568..4587) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5247..6107) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5870..5889 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6108..6212) /gene="bla" /label=AmpR promoter primer_bind 6280..6298 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(6336..6358) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6360..6380) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6365..6695 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6546..6681 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6608..6627 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 6832..6851 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(6918..7373) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7055..7076) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7267..7286 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7499..7521 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 7513..7530 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 7514..7530 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa 61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt 301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac 361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga 721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc 781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc 841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac 901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca 961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta 1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc 1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt 1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc 1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac 1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt 1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta 1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg 1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg 1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg 1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgtggacccc 1621 gcgagtgccc cctccacgcc cggccctgtc gtttttcctg ctgttgctcc tgggggtcac 1681 ctatggcctt ttcccagaag aaccacctcc actcagcgtg gctcccaggg actacttgag 1741 ccactatccc gtgttcgtgg gcagcgggcc tggtcgtctg accgctgcgg agggtgctga 1801 ggacctcaac atccagagag tgctacgtgt taacaggaca ctgttcattg gggacagaga 1861 taacctgtac caagtagaac tggagccatc cacttccacg gagctgcgat atcagaggaa 1921 gctgacctgg cgctccaacc ccagtgacat cgatgtgtgt cgaatgaagg gcaagcaaga 1981 gggtgagtgt cggaactttg tcaaggtgct cctgcttcgc gatgagtcca cgctgttcgt 2041 gtgcggctcc aatgcgttca atcccatctg cgccaattac agtatggaca cactgcagct 2101 tcttggtgac agcatcagcg gtatggcccg ctgcccctat gaccccaaac atgccaatgt 2161 cgccctcttc tcagatggga tgctcttcac agccacggtg actgacttcc tagccatcga 2221 cgctgttatc taccgcagcc ttggggaccg gcccacactg cgcacagtaa agcacgactc 2281 caagtggttt aaagagccgt actttgtgca tgcagtggag tggggaagcc acgtctattt 2341 cttcttccgg gagatcgcca tggagtttaa ctacctggaa aaggtggtgg tgtctcgagt 2401 ggcccgtgtg tgcaagaatg acgtgggcgg ctcgccacga gtgctggaga agcagtggac 2461 ttccttcctg aaggcccggc tcaactgctc agtgccaggg gattcacact tctacttcaa 2521 cgtcctgcag gctgtgacgg gcgtggtgag cctgggtggt cgtccggtga tcctcgctgt 2581 cttctcaact cctagcaaca gcatccccgg ctcagccgtc tgcgcctttg acatgaacca 2641 agtggctgct gtgtttgaag gccgcttccg ggaacagaag tcgcctgagt cgatctggac 2701 accagtgcct gaagaccaag tgccacggcc caggcctggg tgctgcgcgg cacctggcat 2761 gcagtacaac gcgtccagtg ccctccctga cgagatcctc aactttgtaa agacccaccc 2821 actgatggat gaggcagtac cttccctggg tcactcgccg tggattgtga gaactctgat 2881 gcggcaccag ctgacccgag tggctgtgga tgtgggtgcg ggtccatggg gcaatcagac 2941 aatagtcttc cttggctccg aggctggcac agtcctcaaa tttcttgtga agcccaacgc 3001 cagtgtctca gggaccacag ggcccagcat ctttttggag gagtttgaga cctaccggcc 3061 agacaggtgt ggacgaccca gcagtgctgg tgagtggggg cagcgacttc tcagcctgga 3121 gctggatgca gcctcagggg gcctgctggc agccttcccc cgctgtgtgg ttcgtgttcc 3181 tgttgcccgt tgccagctgt actcggggtg catgaagaac tgtattggca gccaggatcc 3241 atactgcggg tgggcccctg atggctcctg catcttcctc agaccaggaa ctagtgccac 3301 gtttgagcaa gatgtgtccg gggccagcac ctctggctta ggtgactgca ctggactctt 3361 gcgcgccagc ctctcagatg accgcgcagg gctggtgtcg gtgaacctgc tagtgacggg 3421 cgcgcctgac aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc 3481 gtcagtcttc ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga 3541 ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta 3601 cgtggacggc gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag 3661 cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga 3721 gtacaagtgc aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa 3781 agccaaaggg cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct 3841 gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc 3901 cgtggagtgg gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct 3961 ggactccgac ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca 4021 gcaggggaac gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca 4081 gaagagcctc tccctgtctc cgggtaaagg cggccatcat caccatcacc attaatctag 4141 agatcttatt aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4201 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 4261 aactcatcaa tgtatcttat catgtctggt cgactctagc tagactcttc cgcttcctcg 4321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 4501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 4561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 4621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 4681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 4741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 4801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 4861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 4921 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 4981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 5041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5221 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5281 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5341 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5401 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 5461 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 5521 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 5581 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 5641 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 5701 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 5761 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 5821 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 5881 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 5941 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6001 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6061 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6241 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 6301 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 6361 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc 6421 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 6481 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 6541 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 6601 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 6661 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 6721 ttttcgtccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 6781 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 6841 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 6901 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 6961 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 7021 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 7081 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 7141 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 7201 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 7261 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 7321 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg 7381 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 7441 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 7501 cagtcacgac gttgtaaaac gacggccagt gaatt //