LOCUS Exported 771 bp ds-DNA linear SYN 03-JUL-2021 DEFINITION CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.. ACCESSION . VERSION . KEYWORDS pdCas9-M-1B6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 771) AUTHORS Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, Linhardt RJ, Koffas MA TITLE Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. JOURNAL Nucleic Acids Res. 2016 Apr 13. pii: gkw231. PUBMED 27079979 REFERENCE 2 (bases 1 to 771) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..771 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 390..425 /label=DR /note="direct repeat for the Streptococcus pyogenes CRISPR/Cas system" repeat_region 456..491 /label=DR /note="direct repeat for the Streptococcus pyogenes CRISPR/Cas system" misc_feature complement(492..623) /label=crRNA leader /note="crRNA leader sequence for the Streptococcus pyogenes CRISPR/Cas system" ORIGIN 1 cgatgcgtcc ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga 61 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg 121 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat 181 agtgactggc gatgctgtcg gaatggacga tcacactact cttcttttgc ctattataac 241 atgaaaacgc tttaacagaa agaataggaa ggtatccgac tgctggtatt aaccctcttt 301 ctcaagttat catcggcaat gttgaatgga gtccattcaa aacagcatag ctctaaaaca 361 tggaactcaa caagtctcag tgtgctgaag ttttgggacc attcaaaaca gcatagctct 421 aaaacattta gtgagtcgta ttaatttcgc gggatgtttt gggaccattc aaaacagcat 481 agctctaaaa cctcgtagac tatttttgtc taaaaaattt cgtaatcgca ctatttgtct 541 cagctagact tcagtcttga aaagcccctg tattactgca tttattaaga gtattatacc 601 atatttttag ttattaagaa ataatcttca tctaaaatat acttcagtca cctcctagct 661 gactcaaatc aatgcgtgtt tcataaagac cagtgatgga ttgatggata agagtggcat 721 ctaaaacttc ttttgtagac gtatatcgtt tacgatcaat tgttgtatca a //