LOCUS Exported 6828 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS p181 pK7-HDAC1 (GFP) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6828) AUTHORS Tou L, Liu Q, Shivdasani RA TITLE Regulation of mammalian epithelial differentiation and intestine development by class I histone deacetylases. JOURNAL Mol Cell Biol. 2004 Apr . 24(8):3132-9. PUBMED 15060137 REFERENCE 2 (bases 1 to 6828) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6828 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" enhancer 250..629 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 630..833 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 784..804 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 830..854 /label=LNCX /note="Human CMV promoter, forward primer" promoter 1064..1082 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1064..1081 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 1095..1114 /label=pMT2-F /note="Synthetic intron, forward primer" primer_bind complement(1202..1230) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 1825..1845 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" CDS 1877..1894 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" regulatory 3364..3373 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 3377..3511 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3414..3433) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3468..3487 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 3580..3937 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3603..3623) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3788..3923 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3850..3869 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(3957..3974) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3957..3973) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3966..3988) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4186..4641 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4273..4292) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4483..4504 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4658..4677) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4777..4799 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4837..4855) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4923..5027 /gene="bla" /label=AmpR promoter CDS 5028..5888 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5246..5265) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6059..6647 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6548..6567 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6801..6818 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaattcg 241 agctcgcccg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 841 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc 901 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca 961 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac 1021 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata 1081 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct 1141 cggttctaag cttctgcagg tcgactctag aatgagtaaa ggagaagaac ttttcactgg 1201 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 1261 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 1321 tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg gtgttcaatg 1381 cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga 1441 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 1501 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 1561 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 1621 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 1681 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 1741 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 1801 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 1861 acatggcatg gatgaactag ttccgcgtgg atccagcaag atggcgcaga ctcagggcac 1921 caagaggaaa gtctgttact actacgacgg ggatgttgga aactactatt atggacaagg 1981 gcaccccatg aagcctcacc gaatccgcat gactcacaat ttgctgctca actatggtct 2041 ctaccgaaaa atggagatct accgtcctca caaagccaat gctgaggaga tgaccaagta 2101 ccacagtgat gactacatta aattcctgcg ttctattcgc ccagataaca tgtctgaata 2161 cagcaagcag atgcagagat tcaatgttgg tgaggactgt ccggtatttg atggcttgtt 2221 tgagttctgt cagttgtcca cgggaggctc tgtcgcaagt gctgtgaagc ttaataagca 2281 gcagacggac atcgctgtga actgggctgg gggcctgcac catgcaaaga agtctgaagc 2341 atccggcttc tgttacgtca atgacatcgt cttggccatc ctggaactgc taaagtacca 2401 ccagagggtg ctctatattg acattgatat tcaccatggc gatggcgtgg aagaggcctt 2461 ctatactaca gaccgggtca tgactgtgtc ctttcataaa tacggagagt acttcccagg 2521 aactggggac ctacgggaca ttggggctgg caaaggcaag tactatgctg tgaactaccc 2581 actgcgagac ggcattgacg acgaatccta tgaagccatc tttaagccag tcatgtccaa 2641 agtaatggag atgttccagc ctagtgcagt ggtcttacag tgtggctcag attccctgtc 2701 tggggaccgg ttaggttgct tcaatctgac catcaaagga cacgccaagt gtgtggagtt 2761 cgtgaagagt ttcaacttgc ccatgctgat gctgggagga ggtggctaca ccatccggaa 2821 tgttgctcgc tgctggactt acgaaacagc ggtggccctg gacacagaga tccctaatga 2881 gctgccctac aatgactact ttgaatactt tggaccggat ttcaagcttc acatcagccc 2941 ttctaacatg accaaccaga acactaacga gtacctggag aagatcaagc agcgtctctt 3001 tgagaacttg aggatgctgc cccatgcccc tggggtccag atgcaggcca tccctgagga 3061 cgccatcccc gaagagagtg gggatgaaga tgaggaggac cctgacaaac gcatctccat 3121 ctgctcctct gataaacgca ttgcctgtga ggaagagttc tcggactcag atgaggaggg 3181 agaaggtggt cgcaagaact cttctaactt caaaaaagcc aaaagagtta aaacagagga 3241 tgagaaagag aaagatcctg aagagaaaaa agaagtcaca gaagaagaga aaaccaagga 3301 ggagaagcca gaagccaaag gggtcaaaga agaggtcaag ttggcctgag aattcaatcg 3361 atggccgcca tggcccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 3421 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 3481 actcatcaat gtatcttatc atgtctggat cgggaattaa ttcggcgcag caccatggcc 3541 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct 3601 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 3661 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 3721 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3781 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 3841 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 3901 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctgttaacag cttggcactg 3961 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 4021 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 4081 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg 4141 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg 4201 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 4261 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 4321 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 4381 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga 4441 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 4501 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga 4561 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 4621 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat 4681 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 4741 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 4801 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 4861 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 4921 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 4981 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 5041 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 5101 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 5161 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 5221 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 5281 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 5341 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 5401 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 5461 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 5521 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 5581 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 5641 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 5701 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 5761 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 5821 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 5881 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 5941 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 6001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 6061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 6121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 6181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 6241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 6301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 6361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 6421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 6481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 6721 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 6781 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag //