LOCUS Exported 8573 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses the extracellular region of the PlexinC1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.. ACCESSION . VERSION . KEYWORDS Plxnc1-Fc-His SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8573) AUTHORS Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray TA, Kay JN, Wojtowicz WM TITLE An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. JOURNAL Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149. PUBMED 26633812 REFERENCE 2 (bases 1 to 8573) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8573 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 105..408 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 409..612 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 563..583 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 609..633 /label=LNCX /note="Human CMV promoter, forward primer" intron 748..1573 /label=CMV intron A /note="human cytomegalovirus intron A (Chapman et al., 1991)" regulatory 1604..1613 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 5153..5170 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" polyA_signal 5196..5317 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5233..5252) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5287..5306 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5355..5372) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5526..6114) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5606..5625) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6285..7145) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6908..6927 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7146..7250) /gene="bla" /label=AmpR promoter primer_bind 7318..7336 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(7374..7396) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7398..7418) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7403..7733 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7584..7719 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7646..7665 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 7870..7889 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(7956..8411) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8093..8114) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 8305..8324 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8537..8559 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 8551..8568 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 8552..8568 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa 61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt 301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac 361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga 721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc 781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc 841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac 901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca 961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta 1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc 1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt 1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc 1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac 1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt 1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta 1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg 1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg 1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg 1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggaggtctc 1621 ccggaggaag acgccgccgc gccccccgta ccccgccgca ccgttgcccc tgatcgccta 1681 tctgctggcg ctggcagcgc ccgcccgggg cgcggacgag ccggtgtggc gatctgagca 1741 agccatcgga gccatcgcgg cgagccgggc ggacggcgtg ttcgtggcga gcggcagctg 1801 cctggaccag ctggactaca gcctgaagaa caggctctct cgcctatacc gggaccaagc 1861 gggcaactgc acggagcccg tctcgttggc gccccccgcg cggccccggc ccgggagcag 1921 cttcagcaag ctactgctgc cctaccgcga gggggcgacc ggcctcgagg ggctgctgct 1981 caccggctgg accttcgacc ggggcgcctg cgaggtgcgg cccctgggca acctgaaccg 2041 cagctccctg cgcaacggca ccgaggtggt gtcatgtcac ccgcagggtt cgacggctgg 2101 tgtggtgtac cgcgcgagtg gcaccgacct ctggtacctg gcggtggccg ccacctatgt 2161 gctgccggag ccggagaccg cgaaccgctg caaccccgca gcatccgatc gcgacacggc 2221 tatcgcgctc aagaacacgg aggggcgcag tctggccacg caggagctgg gccgcctgaa 2281 actgcgcggt agtgcgggca gcctgcactt cgtggacgcc tttctctgga acggcagtgt 2341 ctacttcccc tactatccct acaactacac gagtggcgct gccaccggct ggcccagcat 2401 ggcgcgcatc gcgcagagca cagaggtgct gttccagggc caggctgccc tcgactgcga 2461 ccacggccac cctgagggcc gccgcctact gctttcctcc agcctggtgg aggctgtgga 2521 catctgggcc ggcgtgttca gcgcggccac tggagagggc caggagaggc gctcccccgc 2581 caccacggct ctctgcctct tcaggatgag tgagatccag gcgcacgcca ggagctgcag 2641 ctgggacttt caagcgactg agcacaactg caaagaaggg gatcggcctg aacgggtcca 2701 gccaattgca tcgtctacac tgatccactc tgacctgacg tctgtttatg gcaccgtggt 2761 gatgaacagg actgttctgt tcttggggac cggagatggc cagttactga aggttgttct 2821 tggtgagaat ttgacttcaa actgtccaga ggttatttat gaaattaaag aagaaacgcc 2881 tgtgttctac aagctcgttc ctcatcccat gaagaacatc tacatctacc taacagctgg 2941 gaaggaggtg agaagaattc ctgttgcaaa ctgcagtaaa cggaaatcct gttcagagtg 3001 cttagcagcg gcagaccccc actgtggctg gtgcctcccg ttacaaaggt gcacttttca 3061 aggggattgc acacatgctg ggagctttga aaactggctg gatatttcat caggacctaa 3121 aaaatgcccg aaaattcaga tacttcgaag cctccgagag agaaccacag tgaccattgt 3181 gggaagcatc tccgcaagac actcggagtg cgtggtgaag aacgcagaca cgggcaagct 3241 gctctgccag gggagaagcc aactgaactg gacctgtgcc tgcaatatcc ccagcagacc 3301 cagctataat gtcttagttg tcaatgcgac cttctccttt ccgtcttgga acttatcaga 3361 aagattcaac ttcaccaact gtgcgtcatt aaaagaatgc ccggcgtgta tcagaagcgg 3421 ctgtgcttgg tgtaaacggg acaagaaatg catccacccc ttcaccccct gcgaaccatc 3481 agactatgag aggaatcagg agctctgtca agttgctgtg gagaagtcgc ccaaggactc 3541 aggaggagga agagtcaagg agagcaagag aaacagaact gacggggctg tgcaggtctt 3601 ctacattaag gccattgagc cacaaaaaat atcaacctta gggaaaagca atgtgattgt 3661 tactggagca aacttcaccc aggcgtcaaa tatcaccatg atcctgcgag gaaccagtac 3721 ctgcgagagg gatgtgatcc gggttagcca tgtgctaaat gacacgcata tgaaattctc 3781 acttccatca agccgcaaag aaatgaaaga cgtttgcatc cagtttgacg gtgggacctg 3841 ctcgtctgca ggggctctgt cctacattgc tctgccacat tgttccctga tagttcctgc 3901 caccacctgg atcagcggtg ggcaaaacat aaccatcatg ggcagaaatt ttgacgtcat 3961 cgacaacctc atcatttcac acgagctgaa gggaaacgca aatgtgaaca taaatgtctc 4021 tgaatactgc gcagcaactt tctgcaggtt tctagctcct aatttaaaga gctccaaagt 4081 acgtacaaac gtggccgtga agctgagggt ccaagacacc tacttggact gtggaacgct 4141 gcaatacctc gaggacccca gatttacagg gtaccgagtg gagtcagaga ttgatacaga 4201 attggaagta aaaattcaaa aagaaaatga caatttcaac atttccaagg atgacattga 4261 tattactctc ttccatgggg aaaataaaca atttaattgc agttttgaaa atattactag 4321 aaaccaggat ctcacaacca tcctttgcaa gatcaagagt atcaagaatg caaacaccat 4381 cgctagctcc tctaaaaaag tccgcgtcaa gctgggaaac ctggaactct atgtggaaca 4441 ggaatcagtt ccttccggcg cgcctgacaa aactcacaca tgcccaccgt gcccagcacc 4501 tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat 4561 gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga 4621 ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg 4681 ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga 4741 ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat 4801 cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt ataccctgcc 4861 cccatcccgg gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt 4921 ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa 4981 gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt 5041 ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct 5101 gcacaaccac tacacgcaga agagcctctc cctgtctccg ggtaaaggcg gccatcatca 5161 ccatcaccat taatctagag atcttattaa agcagaactt gtttattgca gcttataatg 5221 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 5281 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg actctagcta 5341 gactcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 5401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 5461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 5521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 5581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 5641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 5701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 5761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 5821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 5881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 5941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 6001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 6121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 6181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 6241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 6301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 6361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 6421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 6481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 6541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 6601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 6661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 6721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 6781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 6841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 6901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 6961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 7141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7261 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 7321 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga 7381 cacatgcagc tcccggagga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 7441 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 7501 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 7561 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 7621 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 7681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 7741 cgggagcttg tatatccatt ttcgtcccgg agacggtcac agcttgtctg taagcggatg 7801 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 7861 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 7921 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt 7981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 8041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 8101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 8161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 8221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 8281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 8341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 8401 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 8461 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 8521 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga att //