LOCUS       Exported                 962 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  C. elegans germline CRE expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    pMLS328
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(364..384)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(396..420)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttttcggatc cgccgcataa ccagtgaaac agcattgctg tcacttggtc gtggcagccc
       61 ggaccgacga tgaagcatgt ttagctggcc caaatgttgc tggatagttt ttactgccag
      121 accgcgcgcc tgaagatata gaagataatc gcgaacatct tcaggttctg cgggaaacca
      181 tttccggtta ttcaacttgc accatgccgc ccacgaccgg caaacggaca gaagcatttt
      241 ccaggtatgc tcagaaaacg cctggcgatc cctgaacatg tccatcaggt tcttgcgaac
      301 ctcatcactc gttgcatcga ccggtaatgc aggcaaattt tggtgtacgg tcagtaaatt
      361 ggacaccttc ctcttcttct tgggtgcgcc catggagcct gcttttttgt acaaacttgt
      421 gagcaaagtg tttcccaact gaaaaaaaaa caatttaatt taaaagtaag aagagtgcgg
      481 acggtagaga gaataaaagt cgaaaaaatt atcctagaaa cttctaatat ttttaaaaat
      541 taaaaaaata taaaaagtga ggtcacaaat aagacagtta gaacatttgt tgaaagccta
      601 aaataaaaat tcgactaaac agtgagaaca ggaaaaacgc tgggactgaa tgtttgacct
      661 cattataaat tcataaaatt taaatttcct aatctggata aaaacaaaga taaaaacaaa
      721 ataaaccaac aaaaaaccaa catgattagt cagatgacca gaaaactcac gttaatttca
      781 caaggcccaa aaaaatctct ccccctctcg ttgctgcctg cacatctaac tcctagcacg
      841 aaaatgtacc gtacaccatt tcaaacactc tcggcgggag tgttgcatac tttttctctc
      901 tggcagttta ttttcaataa attcttcatt tttatattgt ataatgtctt tattttatga
      961 aa
//
