LOCUS Exported 2542 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION SapTrap flexible linker C-terminal connector donor plasmid. ACCESSION . VERSION . KEYWORDS pMLS287 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2542) AUTHORS Schwartz ML, Jorgensen EM TITLE SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. JOURNAL Genetics. 2016 Feb 2. pii: genetics.115.184275. PUBMED 26837755 REFERENCE 2 (bases 1 to 2542) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2542 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 563..582 /label=T7 /note="T7 promoter, forward primer" promoter 563..581 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 606..622 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 607..623 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(715..731) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(715..731) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(768..788) /label=T3 /note="T3 promoter, forward primer" promoter complement(768..786) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(807..823) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(807..823) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(925..944) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 936..1745 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1003..1022) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1892..2480 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2381..2400 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc 601 ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgctcttcg 661 gcgggcagcg gtggcagtgg aggtaccggc ggaagcggta tgcgaagagc gggggatcca 721 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg 781 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctggcagctc tggcccgtgt 841 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac 901 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt 961 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 1021 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag 1081 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 1141 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 1201 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag 1261 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 1321 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc 1381 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta 1441 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg 1501 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 1561 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 1621 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 1681 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt 1741 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg 1801 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt 1861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 2041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 2101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 2161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 2221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 2281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 2341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 2401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 2461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 2521 gctggccttt tgctcacatg tt //