LOCUS Exported 2751 bp ds-DNA linear SYN 06-SEP-2021 DEFINITION Cloning vector containing N2c Glycoprotein and histone GFP. ACCESSION . VERSION . KEYWORDS pcDNA-H2B-EGFP-P2A-N2c(G) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2751) AUTHORS Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, Jessell TM, Losonczy A TITLE Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer and Neuronal Viability. JOURNAL Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 10.1016/j.neuron.2016.01.004. PUBMED 26804990 REFERENCE 2 (bases 1 to 2751) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 6, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2751 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..375 /codon_start=1 /gene="HIST1H2BJ" /product="human histone H2B" /label=H2B /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG ELAKHAVSEGTKAITKYTSAK" regulatory 388..397 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 394..1110 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 394..1110 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(439..460) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(700..719) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1047..1068 /label=EGFP-C /note="EGFP, forward primer" CDS 1120..1176 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" ORIGIN 1 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag 61 gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat 121 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc 181 atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg 241 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg 301 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc catcaccaag 361 tacaccagcg ctaaggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc 421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 481 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 541 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 601 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 661 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 781 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 841 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 901 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 961 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1021 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1081 atcactctcg gcatggacga gctgtacaag ggaagcggag ctactaactt cagcctgctg 1141 aagcaggctg gagacgtgga ggagaaccct ggacctatgg ttcctcaggt tcttttgttt 1201 gtactccttc tgggtttttc gttgtgtttc gggaagttcc ccatttacac gataccagac 1261 gaacttggtc cctggagccc tattgacata caccatctca gctgtccaaa taacctggtt 1321 gtggaggatg aaggatgtac caacctgtcc gagttctcct acatggaact caaagtggga 1381 tacatctcag ccatcaaagt gaacgggttc acttgcacag gtgttgtgac agaggcagag 1441 acctacacca actttgttgg ttatgtcaca accacattca agagaaagca tttccgcccc 1501 accccagacg catgtagagc cgcgtataac tggaagatgg ccggtgaccc cagatatgaa 1561 gagtccctac acaatccata ccccgactac cactggcttc gaactgtaag aaccaccaaa 1621 gagtccctca ttatcatatc cccaagtgtg acagatttgg acccatatga caaatccctt 1681 cactcaaggg tcttccctgg cggaaagtgc tcaggaataa cggtgtcctc tacctactgc 1741 tcaactaacc atgattacac catttggatg cccgagaatc cgagaccaag gacaccttgt 1801 gacattttta ccaatagcag agggaagaga gcatccaacg ggaacaagac ttgcggcttt 1861 gtggatgaaa gaggcctgta taagtctcta aaaggagcat gcaggctcaa gttatgtgga 1921 gttcttggac ttagacttat ggatggaaca tgggtcgcga tgcaaacatc agatgagacc 1981 aaatggtgcc ctccagatca gttggtgaat ttgcacgact ttcgctcaga cgagattgag 2041 catctcgttg tggaggagtt agtcaagaaa agagaggaat gtctggacgc attagagtcc 2101 atcatgacca ccaagtcagt aagtttcaga cgtctcagtc acctgagaaa acttgtccca 2161 gggtttggaa aagcatatac catattcaac aaaaccttga tggaggctga tgctcactac 2221 aagtcagtcc ggacctggaa tgagatcatc ccctcaaaag ggtgtttgaa agttggagga 2281 aggtgccatc ctcatgtgaa cggggtgttt ttcaatggta taatattagg gcctgacgac 2341 catgtcctaa tcccagagat gcaatcatcc ctcctccagc aacatatgga gttgttggaa 2401 tcttcagtta tccccctgat gcaccccctg gcagaccctt ctacagtttt caaagaaggt 2461 gatgaggctg aggattttgt tgaagttcac ctccccgatg tgtacaaaca gatctcaggg 2521 gttgacctgg gtctcccgaa ctggggaaag tatgtattga tgactgcagg ggccatgatt 2581 ggcctggtgt tgatattttc cctaatgaca tggtgcagaa gagccaatcg accagaatcg 2641 aaacaacgca gttttggagg gacagggggg aatgtgtcag tcacttccca aagcggaaaa 2701 gtcatacctt catgggaatc atataagagt ggaggtgaga tcagactgtg a //