LOCUS Exported 6367 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION JxOn-pre. ACCESSION . VERSION . KEYWORDS paavCAG-JxON-pre-mGRASP-mCerulean SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6367) AUTHORS Druckmann S, Feng L, Lee B, Yook C, Zhao T, Magee JC, Kim J TITLE Structured Synaptic Connectivity between Hippocampal Regions. JOURNAL Neuron. 2014 Feb 5;81(3):629-40. doi: 10.1016/j.neuron.2013.11.026. Epub 2014 Jan 9. PUBMED 24412418 REFERENCE 2 (bases 1 to 6367) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6367 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..278 /label=chicken beta-actin promoter primer_bind 557..579 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 641..660 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind 692..725 /label=lox66 /bound_moiety="Cre recombinase" /note="Right element (RE) mutant of loxP (Araki et al., 2010). Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(2325..2372) /codon_start=1 /product="11th beta-strand of superfolder GFP" /label=GFP11 /note="associates with GFP(1-10), consisting of the first 10 beta-strands, to reconstitute fluorescent GFP (Cabantous et al., 2005; Kamiyama et al., 2016)" /translation="RDHMVLHEYVNAAGIT" protein_bind complement(2472..2505) /label=lox71 /bound_moiety="Cre recombinase" /note="Left element (LE) mutant of loxP (Araki et al., 2010). Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 2518..3106 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2571..2591) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2989..3000) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3149..3270 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3160..3179) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3214..3233 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(3478..3495) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3649..4237) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3729..3748) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4408..5268) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5031..5050 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5269..5373) /gene="bla" /label=AmpR promoter rep_origin complement(5399..5854) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5536..5557) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5748..5767 /label=F1ori-R /note="F1 origin, reverse primer" repeat_region 5926..6055 /label=AAV2 ITR ORIGIN 1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa 61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg 121 ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg 181 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc 241 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc 301 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt 361 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg 421 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg 481 gccctttgtg cggggggagc ggctcggggc tgtccgcggg gggacggctg ccttcggggg 541 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct 601 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg 661 ctgtctcatc attttggcaa agaattggat cataacttcg tatagcatac attatacgaa 721 cggtaaagct tgatgtcaaa ctctcacttg tacagctcat ccatacccag ggttatgccg 781 gcagctgtga caaactccag cagtaccata tgatctctct tttcgttggg gtccttgctc 841 agcttgctct gtgtactaag gtagtgattg tcgggcaaca gcacaggtcc atcaccgatg 901 ggagtattct gttggtaatg gtcagccagc tgaacgcttc catcctctat gttgtgccgg 961 attttgaaat tggcctttat gccatttttc tgcttgtcgg cggttatgta aacattatca 1021 gatatggcgt tgtattccag cttgtggccg agaatgttcc catcttcttt gaagtcgatt 1081 cccttgagtt caatcctgtt caccaaggtg tcaccttcga atttcacctc cgcccttgtc 1141 ttgtagttac catcgtcctt gaaaaatatc gtcctttcct gaacataccc ctcgggcata 1201 gcggacttga agaagtcgtg ctgcttcatg tggtcagggt accgggcgaa gcactgtaca 1261 ccccatgtga gggtggtgac gagtgtgggc caaggcacgg ggagcttccc tgttgtacag 1321 atgaacttca gcgtcagttt gccgtaggtg gcatcccctt ctccctcgcc gcttacggag 1381 aacttgtgcc catttacatc tccgtcgagc tccaccagga taggcacgac ccctgtaaac 1441 aactcttcgc ccttgctcac catagatctt acgtagtact ctttatcctt gtttttcttg 1501 tttttgtttg ctgatttggc gctggagggc tgtttctctt taaccacggc gccattactc 1561 tgagcgctgt tactgatata gttcctagat tcgtcaacgt ggtaagatcc ctcatccctg 1621 ttgcgatact tgtagcgtac gcagaagaag atgcccaggc cgataaacag aagcaggccg 1681 gcgactcccc cgagtacgat cagggccatc ggctgtactg gtgttgacca agtaggaagc 1741 accttgatgt tgctctccag cagcacctgt ccagagtcgc taagaagaca ctgccacatc 1801 cctgcctccg ggttaaggac ccacacagcc ttctctcttt tactgacttt agcctcctta 1861 ttctccagct tcagagacag catcagttta ggtgaggtgg gaccccacac ttcgcaggtc 1921 aggttcttct gcagctgtgt tgccctcatc accacgaggt tgacctcctg atgcaacttt 1981 ccggtcttag cctccaaggc gagggtgagg ttcccggagc cagcatactg gggcagggct 2041 tgggggagtg tcaagtgcaa aggcaacttc ttgcccatct gcagcttagg gtcctgtgtg 2101 actctcttca cgctcacttc tttgtttttc agatcaaaag tgatccaact cttgctggag 2161 gaggctcttt cggcctgcca ccacaactct cctgatcctg tgagcttctc gactgtgaag 2221 gccaatggga aactgaactc cacctgttcc ccctccttct tataaacaat agagctagct 2281 ttctgaaagt cgacgctacc accgccgcca gatccgccac cgcctgtgat gccagcagcg 2341 ttcacatatt cgtgcagcac catgtggtcg cgggaagcgg tgacttctcc tgtcttccaa 2401 tctgaagcgg gaagagcgaa ggggagaagt gcagcaagca acaaaagact agtagaaggt 2461 ggcatggatc tataacttcg tataatgtat gctatacgaa cggtacagct tatcgataat 2521 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 2581 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 2641 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 2701 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 2761 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 2821 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 2881 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 2941 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 3001 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 3061 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga 3121 cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca 3181 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt 3241 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt 3301 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt 3361 aaaatcgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat 3421 cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc 3481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 3961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 4081 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 4141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 4201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 4741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 4801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 4861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 4921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 4981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 5041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa 5401 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt 5461 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata 5521 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 5581 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa 5641 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc 5701 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 5761 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 5821 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac 5881 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctgcg cgctcgctcg 5941 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca 6001 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag 6061 ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa gatcgtacca 6121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 6181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 6241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 6301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 6361 accatgg //