LOCUS       Exported                 722 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2.
ACCESSION   .
VERSION     .
KEYWORDS    pX330-sgRNA_Ago2_1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, 
            Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, 
            Cohen-Tannoudji M, Ciaudo C
  TITLE     Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation
            of Mouse Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023.
  PUBMED    29396181
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        208..283
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        380..665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacaccgtac cctgcccccg tagaggagtt ttagagctag aaatagcaag ttaaaataag
      241 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
      301 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
      361 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      421 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
      481 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      541 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
      601 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      661 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      721 cc
//
