LOCUS Exported 841 bp ds-DNA linear SYN 12-JUN-2021 DEFINITION Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2. ACCESSION . VERSION . KEYWORDS pX330-sgRNA_Ago2_1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 841) AUTHORS Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, Cohen-Tannoudji M, Ciaudo C TITLE Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. JOURNAL Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023. PUBMED 29396181 REFERENCE 2 (bases 1 to 841) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..841 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(3..50) /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" ORIGIN 1 tactttttct tttttgcctg gccggccttt ttcgtggccg ccggcctttt gtcgcctccc 61 agctgagaca ggtcgatccg tgtctcgtac aggccggtga tgctctggtg gatcagggtg 121 gcgtccagca cctctttggt gctggtgtac ctcttccggt cgatggtggt gtcaaagtac 181 ttgaaggcgg caggggctcc cagattggtc agggtaaaca ggtggatgat attctcggcc 241 tgctctctga tgggcttatc ccggtgcttg ttgtaggcgg acagcacttt gtccagatta 301 gcgtcggcca ggatcactct cttggagaac tcgctgatct gctcgatgat ctcgtccagg 361 tagtgcttgt gctgttccac aaacagctgt ttctgctcat tatcctcggg ggagcccttc 421 agcttctcat agtggctggc caggtacagg aagttcacat atttggaggg cagggccagt 481 tcgtttccct tctgcagttc gccggcagag gccagcattc tcttccggcc gttttccagc 541 tcgaacaggg agtacttagg cagcttgatg atcaggtcct ttttcacttc tttgtagccc 601 ttggcttcca gaaagtcgat gggattcttc tcgaagctgc ttctttccat gatggtgatc 661 cccagcagct ctttcacact cttcagtttc ttggacttgc ccttttccac tttggccacc 721 accagcacag aataggccac ggtggggctg tcgaagccgc cgtacttctt agggtcccag 781 tccttctttc tggcgatcag cttatcgctg ttcctcttgg gcaggataga ctctttgctg 841 a //