LOCUS Exported 6562 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner. ACCESSION . VERSION . KEYWORDS pAAV-CAG-fDIO-oG-WPRE-SV40pA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6562) AUTHORS Kim EJ, Jacobs MW, Ito-Cole T, Callaway EM TITLE Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies Virus Glycoproteins JOURNAL Cell Reports 2016 REFERENCE 2 (bases 1 to 6562) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6562 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(11..28) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(182..770) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(262..281) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(941..1801) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1564..1583 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1802..1906) /gene="bla" /label=AmpR promoter rep_origin complement(1932..2387) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2069..2090) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2281..2300 /label=F1ori-R /note="F1 origin, reverse primer" repeat_region 2459..2588 /label=AAV2 ITR promoter 2901..3178 /label=chicken beta-actin promoter primer_bind 3458..3480 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 3542..3561 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 5294..5303 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 5464..6052 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5517..5537) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5935..5946) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 6095..6216 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6106..6125) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 6160..6179 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 6378..6507 /label=AAV2 ITR ORIGIN 1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt 2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg 2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag 2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag 2641 gaagatcgta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 2701 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 2761 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 2821 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 2881 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 2941 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 3001 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg 3061 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 3121 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 3181 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc 3241 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc 3301 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa 3361 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg 3421 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc 3481 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg 3541 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tcctccgaag 3601 ttcctattct tcaaatagta taggaacttc gcagaatggt agctggattg tagctgctat 3661 tagcaatatg aaacctctta gaagttccta ttcttcaaaa ggtataggaa cttcggcgcg 3721 ccttagagcc gtgtctcgcc cccagattta tgactttccc aactgctgat gattttaccg 3781 ctctggggtg tgacagacac ttctctaccg gtccccctca gattgtgctg agttggctcg 3841 gagcggttca ctcgtcgcca gcaggtcatc aggaaaatga tcagcatgag agcagtcagt 3901 gctccagcgg agagcagcac gtacttgccc caattaggca gtccgagatc gacgccactg 3961 atccgttcgt gcacgtcggg caggtggacc tccacaaaat cttcggcctc gtcgccgttt 4021 ttgaacactg tgcttgggtc tgcgagagga tgcatcaggg gaatgacgct tgacacgagc 4081 agttccatgt gctgctggag cagactagac tgcatctcgg ggatcagcac gttaccatct 4141 ggccccagaa tgataccatt aaagaagacc ccgttcacat gtgggtgaca tcgacccccg 4201 actcgcaggc atcccttact aggaatgatt tcattccagg ttctcacaga tttataatgg 4261 gcgtctgcct ccatcagtgt cttgttaaag atggtgtatg cttttccgaa gccagggacg 4321 agcttcctca ggtggctcag cctcctgaag ctcacgctct tggtagtcat aattgactcc 4381 agagcatcga gacattcctc ccttttcttc acgagttcct cgaccaccag atgttcgatc 4441 tcatcgcttc ggaagtcgtg cagattcacg agctgcccag gggggcacca cttagtctcg 4501 ttagatgtct gcatagcgac ccaggttccg tccatcagcc ggagtcccag cacgccacag 4561 agtttcagct tgcaggcacc tttgaggctc ttatacagcc ctctttcatc cacaaatcca 4621 caagtctcgc tgccttttga ggccctcttg cccctgctgt tggtgaagat gtcgcaggac 4681 atccccagcc ttggattctc aggcatccag attgtataat catggttggt ggaacagtaa 4741 gtggagctga cggccactcc ggagcagttt ccgccgggaa acactggact gtggagagat 4801 cggtcgtagg ggtccaggtc ggcgacagat ggggaaatga tgaccaggct ctcttttgtg 4861 gtcttcacag tgcgcagcca atgatagtct gggtaaggat tgtgcaggct ttcctcatag 4921 cgggggtcgc cagccatctt ccagttgtaa gctgcccgac aggcgtctgg tgttggccga 4981 aagtgttttc tcttgaaagt tgtggtgaca tatcccacaa aatttgtgta ggtttctgcc 5041 tcggtgacca caccagtgca tgtgaacccg ttcatcttaa tagcagagat atagcccact 5101 ttcagctcca tgtaggagaa tccgctcagg ttggtacagc cttcgtcctc gaccacgaga 5161 ttgttagggc atgacagatg gtgaatatcg ataggactcc aggggcccag cttgtcgggg 5221 atagtgtaga tagggaattt accaaagcag agtgggaaga cgagcagagg gacgaagagg 5281 agagcctgtg ggaccatggt ggcggcgcta gcactagaga agttcctata ctatttgaag 5341 aataggaact tcttgcctta acccagaaat tatcactgtt attctttaga atggtgcaaa 5401 gagaagttcc tatacctttt gaagaatagg aacttcgaag aattcgatat caagcttatc 5461 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 5521 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 5581 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 5641 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 5701 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 5761 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 5821 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 5881 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 5941 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 6001 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac 6061 cgtcgacccg ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa 6121 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 6181 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 6241 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa 6301 tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg 6361 gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc 6421 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc 6481 ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa tcggccaacg cgcggggaga 6541 ggcggtttgc gtattgggcg ct //