LOCUS Exported 830 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Human OCT4 genomic targeting donor. ACCESSION . VERSION . KEYWORDS OCT4-2A-eGFP-PGK-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 830) AUTHORS Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R TITLE Genetic engineering of human pluripotent cells using TALE nucleases. JOURNAL Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927. PUBMED 21738127 REFERENCE 2 (bases 1 to 830) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..830 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 55..654 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(55..74) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" ORIGIN 1 tctccgggcc tttcgacctg catccatcta gatctcgagc agctgaagct taccatgacc 61 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 121 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 181 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 241 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 301 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 361 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 421 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 481 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 541 tccgcgcccc acaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 601 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg 661 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gatgatatca 721 gatccccggg atgcagaaat tgatgatcta ttaaacaata aagatgtcca ctaaaatgga 781 agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca //