LOCUS       Exported                 809 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Photoactivatable transcription system. Expression of genomic anchor 
            probe, containing dCas9 and CIB1.
ACCESSION   .
VERSION     .
KEYWORDS    NLS-dCas9-trCIB1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             97..606
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acggtaccgg aggaagtggc
       61 agctctggcg gcagtggagg gtctggtggc agcggaatga atggagctat aggaggtgac
      121 cttttgctca attttcctga catgtcggtc ctagagcgcc aaagggctca cctcaagtac
      181 ctcaatccca cctttgattc tcctctcgcc ggcttctttg ccgattcttc aatgattacc
      241 ggcggcgaga tggacagcta tctttcgact gccggtttga atcttccgat gatgtacggt
      301 gagacgacgg tggaaggtga ttcaagactc tcaatttcgc cggaaacgac gcttgggact
      361 ggaaatttca agaaacggaa gtttgataca gagactaagg attgtaatga gaagaagaag
      421 aagatgacga tgaacagaga tgacctagta gaagaaggag aagaagagaa gtcgaaaata
      481 acagagcaaa acaatgggag cacaaaaagc atcaagaaga tgaaacacaa agccaagaaa
      541 gaagagaaca atttctctaa tgattcatct aaagtgacga aggaattgga gaaaacggat
      601 tatattcatg ttcgtgcacg acgaggccaa gccactgata gtcacagcat agcagaacga
      661 gttagaagag aaaagatcag tgagagaatg aagtttctac aagatttggt tcctggatgc
      721 gacaagatca caggcaaagc agggatgctt gatgaaatca ttaactatgt tcagtctctt
      781 cagagacaaa tcgagttctt atcgatgaa
//
