LOCUS Exported 13661 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS 1136- Desmoplakin-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13661) AUTHORS Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ TITLE Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. JOURNAL J Cell Biol. 2005 Dec 19;171(6):1045-59. PUBMED 16365169 REFERENCE 2 (bases 1 to 13661) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13661 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 9608..9617 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 9614..10333 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 9614..10330 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(9659..9680) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(9920..9939) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 10267..10288 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 10456..10577 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10493..10512) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10547..10566 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(10584..11039) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10721..10742) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 10933..10952 /label=F1ori-R /note="F1 origin, reverse primer" promoter 11066..11170 /gene="bla" /label=AmpR promoter promoter 11172..11529 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(11195..11215) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 11380..11515 /label=SV40 ori /note="SV40 origin of replication" primer_bind 11442..11461 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 11564..12358 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(11618..11637) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 12228..12247 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(12546..12565) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 12590..12637 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 12966..13554 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 13455..13474 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgcggccgcg tcaacagccc 661 ctgctccttg ggcccctcca tgccatgccg taatctctcc cacccgacca acaccaacac 721 ccagctccga cgcagctcct ctgcgccctt gccgccctcc gagccacagc tttcctcccg 781 ctcctgcccc cggcccgtcg ccgtctccgc gctcgcagcg gcctcgggag ggcccaggta 841 gcgagcagcg acctcgcgag ccttccgcac tcccgcccgg ttccccggcc gtccgcctat 901 ccttggcccc ctccgctttc tccgcgccgg cccgcctcgc ttatgcctcg gcgctgagcc 961 gctctcccga ttgcccgccg acatgagctg caacggaggc tcccacccgc ggatcaacac 1021 tctgggccgc atgatccgcg ccgagtctgg cccggacctg cgctacgagg tgaccagcgg 1081 cggcgggggc accagcagga tgtactattc tcggcgcggc gtgatcaccg accagaactc 1141 ggacggctac tgtcaaaccg gcacgatgtc caggcaccag aaccagaaca ccatccagga 1201 gctgctgcag aactgctccg actgcttgat gcgagcagag ctcatcgtgc agcctgaatt 1261 gaagtatgga gatggaatac aactgactcg gagtcgagaa ttggatgagt gttttgccca 1321 ggccaatgac caaatggaaa tcctcgacag cttgatcaga gagatgcggc agatgggcca 1381 gccctgtgat gcttaccaga aaaggcttct tcagctccaa gagcaaatgc gagcccttta 1441 taaagccatc agtgtccctc gagtccgcag ggccagctcc aagggtggtg gaggctacac 1501 ttgtcagagt ggctctggct gggatgagtt caccaaacat gtcaccagtg aatgtttggg 1561 gtggatgagg cagcaaaggg cggagatgga catggtggcc tggggtgtgg acctggcctc 1621 agtggagcag cacattaaca gccaccgggg catccacaac tccatcggcg actatcgctg 1681 gcagctggac aaaatcaaag ccgacctgcg cgagaaatct gcgatctacc agttggagga 1741 ggagtatgaa aacctgctga aagcgtcctt tgagaggatg gatcacctgc gacagctgca 1801 gaacatcatt caggccacgt ccagggagat catgtggatc aatgactgcg aggaggagga 1861 gctgctgtac gactggagcg acaagaacac caacatcgct cagaaacagg aggccttctc 1921 catacgcatg agtcaactgg aagttaaaga aaaagagctc aataagctga aacaagaaag 1981 tgaccaactt gtcctcaatc agcatccagc ttcagacaaa attgaggcct atatggacac 2041 tctgcagacg cagtggagtt ggattcttca gatcaccaag tgcattgatg ttcatctgaa 2101 agaaaatgct gcctactttc agttttttga agaggcgcag tctactgaag catacctgaa 2161 ggggctccag gactccatca ggaagaagta cccctgcgac aagaacatgc ccctgcagca 2221 cctgctggaa cagatcaagg agctggagaa agaacgagag aaaatccttg aatacaagcg 2281 tcaggtgcag aacttggtaa acaagtctaa gaagattgta cagctgaagc ctcgtaaccc 2341 agactacaga agcaataaac ccattattct cagagctctc tgtgactaca aacaagatca 2401 gaaaatcgtg cataaggggg atgagtgtat cctgaaggac aacaacgagc gcagcaagtg 2461 gtacgtgacg ggcccgggag gcgttgacat gcttgttccc tctgtggggc tgatcatccc 2521 tcctccgaac ccactggccg tggacctctc ttgcaagatt gagcagtact acgaagccat 2581 cttggctctg tggaaccagc tctacatcaa catgaagagc ctggtgtcct ggcactactg 2641 catgattgac atagagaaga tcagggccat gacaatcgcc aagctgaaaa caatgcggca 2701 ggaagattac atgaagacga tagccgacct tgagttacat taccaagagt tcatcagaaa 2761 tagccaaggc tcagagatgt ttggagatga tgacaagcgg aaaatacagt ctcagttcac 2821 cgatgcccag aagcattacc agaccctggt cattcagctc cctggctatc cccagcacca 2881 gacagtgacc acaactgaaa tcactcatca tggaacctgc caagatgtca accataataa 2941 agtaattgaa accaacagag aaaatgacaa gcaagaaaca tggatgctga tggagctgca 3001 gaagattcgc aggcagatag agcactgcga gggcaggatg actctcaaaa acctccctct 3061 agcagaccag gggtcttctc accacatcac agtgaaaatt aacgagctta agagtgtgca 3121 gaatgattca caagcaattg ctgaggttct caaccagctt aaagatatgc ttgccaactt 3181 cagaggttct gaaaagtact gctatttaca gaatgaagta tttggactat ttcagaaact 3241 ggaaaatatc aatggtgtta cagatggcta cttaaatagc ttatgcacag taagggcact 3301 gctccaggct attctccaaa cagaagacat gttaaaggtt tatgaagcca ggctcactga 3361 ggaggaaact gtctgcctgg acctggataa agtggaagct taccgctgtg gactgaagaa 3421 aataaaaaat gacttgaact tgaagaagtc gttgttggcc actatgaaga cagaactaca 3481 gaaagcccag cagatccact ctcagacttc acagcagtat ccactttatg atctggactt 3541 gggcaagttc ggtgaaaaag tcacacagct gacagaccgc tggcaaagga tagataaaca 3601 gatcgacttt agattatggg acctggagaa acaaatcaag caattgagga attatcgtga 3661 taactatcag gctttctgca agtggctcta tgatgctaaa cgccgccagg attccttaga 3721 atccatgaaa tttggagatt ccaacacagt catgcggttt ttgaatgagc agaagaactt 3781 gcacagtgaa atatctggca aacgagacaa atcagaggaa gtacaaaaaa ttgctgaact 3841 ttgcgccaat tcaattaagg attatgagct ccagctggcc tcatacacct caggactgga 3901 aactctgctg aacataccta tcaagaggac catgattcag tccccttctg gggtgattct 3961 gcaagaggct gcagatgttc atgctcggta cattgaacta cttacaagat ctggagacta 4021 ttacaggttc ttaagtgaga tgctgaagag tttggaagat ctgaagctga aaaataccaa 4081 gatcgaagtt ttggaagagg agctcagact ggcccgagat gccaactcgg aaaactgtaa 4141 taagaacaaa ttcctggatc agaacctgca gaaataccag gcagagtgtt cccagttcaa 4201 agcgaagctt gcgagcctgg aggagctgaa gagacaggct gagctggatg ggaagtcggc 4261 taagcaaaat ctagacaagt gctacggcca aataaaagaa ctcaatgaga agatcacccg 4321 actgacttat gagattgaag atgaaaagag aagaagaaaa tctgtggaag acagatttga 4381 ccaacagaag aatgactatg accaactgca gaaagcaagg caatgtgaaa aggagaacct 4441 tggttggcag aaattagagt ctgagaaagc catcaaggag aaggagtacg agattgaaag 4501 gttgagggtt ctactgcagg aagaaggcac ccggaagaga gaatatgaaa atgagctggc 4561 aaaggtaaga aaccactata atgaggagat gagtaattta aggaacaagt atgaaacaga 4621 gattaacatt acgaagacca ccatcaagga gatatccatg caaaaagagg atgattccaa 4681 aaatcttaga aaccagcttg atagactttc aagggaaaat cgagatctga aggatgaaat 4741 tgtcaggctc aatgacagca tcttgcaggc cactgagcag cgaaggcgag ctgaagaaaa 4801 cgcccttcag caaaaggcct gtggctctga gataatgcag aagaagcagc atctggagat 4861 agaactgaag caggtcatgc agcagcgctc tgaggacaat gcccggcaca agcagtccct 4921 ggaggaggct gccaagacca ttcaggacaa aaataaggag atcgagagac tcaaagctga 4981 gtttcaggag gaggccaagc gccgctggga atatgaaaat gaactgagta aggtaagaaa 5041 caattatgat gaggagatca ttagcttaaa aaatcagttt gagaccgaga tcaacatcac 5101 caagaccacc atccaccagc tcaccatgca gaaggaagag gataccagtg gctaccgggc 5161 tcagatagac aatctcaccc gagaaaacag gagcttatct gaagaaataa agaggctgaa 5221 gaacactcta acccagacca cagagaatct caggagggtg gaagaagaca tccaacagca 5281 aaaggccact ggctctgagg tgtctcagag gaaacagcag ctggaggttg agctgagaca 5341 agtcactcag atgcgaacag aggagagcgt aagatataag caatctcttg atgatgctgc 5401 caaaaccatc caggataaaa acaaggagat agaaaggtta aaacaactga tcgacaaaga 5461 aacaaatgac cggaaatgcc tggaagatga aaacgcgaga ttacaaaggg tccagtatga 5521 cctgcagaaa gcaaacagta gtgcgacgga gacaataaac aaactgaagg ttcaggagca 5581 agaactgaca cgcctgagga tcgactatga aagggtttcc caggagagga ctgtgaagga 5641 ccaggatatc acgcggttcc agaactctct gaaagagctg cagctgcaga agcagaaggt 5701 ggaagaggag ctgaatcggc tgaagaggac cgcgtcagaa gactcctgca agaggaagaa 5761 gctggaggaa gagctggaag gcatgaggag gtcgctgaag gagcaagcca tcaaaatcac 5821 caacctgacc cagcagctgg agcaggcatc cattgttaag aagaggagtg aggatgacct 5881 ccggcagcag agggacgtgc tggatggcca cctgagggaa aagcagagga cccaggaaga 5941 gctgaggagg ctctcttctg aggtcgaggc cctgaggcgg cagttactcc aggaacagga 6001 aagtgtcaaa caagctcact tgaggaatga gcatttccag aaggcgatag aagataaaag 6061 cagaagctta aatgaaagca aaatagaaat tgagaggctg cagtctctca cagagaacct 6121 gaccaaggag cacttgatgt tagaagaaga actgcggaac ctgaggctgg agtacgatga 6181 cctgaggaga ggacgaagcg aagcggacag tgataaaaat gcaaccatct tggaactaag 6241 gagccagctg cagatcagca acaaccggac cctggaactg caggggctga ttaatgattt 6301 acagagagag agggaaaatt tgagacagga aattgagaaa ttccaaaagc aggctttaga 6361 ggcatctaat aggattcagg aatcaaagaa tcagtgtact caggtggtac aggaaagaga 6421 gagccttctg gtgaaaatca aagtcctgga gcaagacaag gcaaggctgc agaggctgga 6481 ggatgagctg aatcgtgcaa aatcaactct agaggcagaa accagggtga aacagcgcct 6541 ggagtgtgag aaacagcaaa ttcagaatga cctgaatcag tggaagactc aatattcccg 6601 caaggaggag gctattagga agatagaatc ggaaagagaa aagagtgaga gagagaagaa 6661 cagtcttagg agtgagatcg aaagactcca agcagagatc aagagaattg aagagaggtg 6721 caggcgtaag ctggaggatt ctaccaggga gacacagtca cagttagaaa cagaacgctc 6781 ccgatatcag agggagattg ataaactcag acagcgccca tatgggtccc atcgagagac 6841 ccagactgag tgtgagtgga ccgttgacac ctccaagctg gtgtttgatg ggctgaggaa 6901 gaaggtgaca gcaatgcagc tctatgagtg tcagctgatc gacaaaacaa ccttggacaa 6961 actattgaag gggaagaagt cagtggaaga agttgcttct gaaatccagc cattccttcg 7021 gggtgcagga tctatcgctg gagcatctgc ttctcctaag gaaaaatact ctttggtaga 7081 ggccaagaga aagaaattaa tcagcccaga atccacagtc atgcttctgg aggcccaggc 7141 agctacaggt ggtataattg atccccatcg gaatgagaag ctgactgtcg acagtgccat 7201 agctcgggac ctcattgact tcgatgaccg tcagcagata tatgcagcag aaaaagctat 7261 cactggtttt gatgatccat tttcaggcaa gacagtatct gtttcagaag ccatcaagaa 7321 aaatttgatt gatagagaaa ccggaatgcg cctgctggaa gcccagattg cttcaggggg 7381 tgtagtagac cctgtgaaca gtgtcttttt gccaaaagat gtcgccttgg cccgggggct 7441 gattgataga gatttgtatc gatccctgaa tgatccccga gatagtcaga aaaactttgt 7501 ggatccagtc accaaaaaga aggtcagtta cgtgcagctg aaggaacggt gcagaatcga 7561 accacatact ggtctgctct tgctttcagt acagaagaga agcatgtcct tccaaggaat 7621 cagacaacct gtgaccgtca ctgagctagt agattctggt atattgagac cgtccactgt 7681 caatgaactg gaatctggtc agatttctta tgacgaggtt ggtgagagaa ttaaggactt 7741 cctccagggt tcaagctgca tagcaggcat atacaatgag accacaaaac agaagcttgg 7801 catttatgag gccatgaaaa ttggcttagt ccgacctggt actgctctgg agttgctgga 7861 agcccaagca gctactggct ttatagtgga tcctgttagc aacttgaggt taccagtgga 7921 ggaagcctac aagagaggtc tggtgggcat tgagttcaaa gagaagctcc tgtctgcaga 7981 acgagctgtc actgggtata atgatcctga aacaggaaac atcatctctt tgttccaagc 8041 catgaataag gaactcatcg aaaagggcca cggtattcgc ttattagaag cacagatcgc 8101 aaccgggggg atcattgacc caaaggagag ccatcgttta ccagttgaca tagcatataa 8161 gaggggctat ttcaatgagg aactcagtga gattctctca gatccaagtg atgataccaa 8221 aggatttttt gaccccaaca ctgaagaaaa tcttacctat ctgcaactaa aagaaagatg 8281 cattaaggat gaggaaacag ggctctgtct tctgcctctg aaagaaaaga agaaacaggt 8341 gcagacatca caaaagaata ccctcaggaa gcgtagagtg gtcatagttg acccagaaac 8401 caataaagaa atgtctgttc aggaggccta caagaagggc ctaattgatt atgaaacctt 8461 caaagaactg tgtgagcagg aatgtgaatg ggaagaaata accatcacgg gatcagatgg 8521 ctccaccagg gtggtcctgg tagatagaaa gacaggcagt cagtatgata ttcaagatgc 8581 tattgacaag ggccttgttg acaggaagtt ctttgatcag taccgatccg gcagcctcag 8641 cctcactcaa tttgctgaca tgatctcctt gaaaaatggt gtcggcacca gcagcagcat 8701 gggcagtggt gtcagcgatg atgtttttag cagctcccga catgaatcag taagtaagat 8761 ttccaccata tccagcgtca ggaatttaac cataaggagc agctcttttt cagacaccct 8821 ggaagaatcg agccccattg cagccatctt tgacacagaa aacctggaga aaatctccat 8881 tacagaaggt atagagcggg gcatcgttga cagcatcacg ggtcagaggc ttctggaggc 8941 tcaggcctgc acaggtggca tcatccaccc aaccacgggc cagaagctgt cacttcagga 9001 cgcagtctcc cagggtgtga ttgaccaaga catggccacc aggctgaagc ctgctcagaa 9061 agccttcata ggcttcgagg gtgtgaaggg aaagaagaag atgtcagcag cagaggcagt 9121 gaaagaaaaa tggctcccgt atgaggctgg ccagcgcttc ctggagttcc agtacctcac 9181 gggaggtctt gttgacccgg aagtgcatgg gaggataagc accgaagaag ccatccggaa 9241 ggggttcata gatggccgcg ccgcacagag gctgcaagac accagcagct atgccaaaat 9301 cctgacctgc cccaaaacca aattaaaaat atcctataag gatgccataa atcgctccat 9361 ggtagaagat atcactgggc tgcgccttct ggaagccgcc tccgtgtcgt ccaagggctt 9421 acccagccct tacaacatgt cttcggctcc ggggtcccgc tccggctccc gctcgggatc 9481 tcgctccgga tctcgctccg ggtcccgcag tgggtcccgg agaggaagct ttgacgccac 9541 agggaattct tcctactctt attcctactc atttagcagt agttctattg ggcacgatcc 9601 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 9661 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 9721 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 9781 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 9841 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 9901 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 9961 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 10021 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 10081 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 10141 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 10201 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 10261 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 10321 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag 10381 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 10441 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 10501 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 10561 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 10621 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 10681 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 10741 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 10801 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 10861 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 10921 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 10981 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 11041 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 11101 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 11161 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 11221 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 11281 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 11341 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 11401 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 11461 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 11521 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 11581 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 11641 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 11701 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 11761 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 11821 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 11881 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 11941 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 12001 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 12061 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 12121 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 12181 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 12241 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 12301 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 12361 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 12421 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 12481 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 12541 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 12601 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 12661 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 12721 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 12781 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 12841 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 12901 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 12961 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 13021 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 13081 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 13141 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 13201 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 13261 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 13321 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 13381 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 13441 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 13501 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 13561 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 13621 cctgcgttat cccctgattc tgtggataac cgtattaccg c //