LOCUS       Exported                 880 bp ds-DNA     linear   SYN 01-JUN-2021
DEFINITION  Expresses mCherry and Cre for monosynaptic circuit mapping and 
            genetic manipulation.
ACCESSION   .
VERSION     .
KEYWORDS    RabV CVS-N2c(deltaG)-mCherry-P2A-Cre
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(92..148)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(158..865)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(433..452)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     702..720
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      862..871
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 acatgtccat caggttcttg cgaacctcat cactcgttgc atcgaccggt aatgcaggca
       61 aattttggtg tacggtcagt aaattggaca taggtccagg gttctcctcc acgtctccag
      121 cctgcttcag caggctgaag ttagtagctc cgcttccctt gtacagctcg tccatgccgc
      181 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
      241 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
      301 tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc
      361 tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct
      421 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
      481 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
      541 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
      601 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact
      661 gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc ttcagcttgg
      721 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
      781 cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt
      841 tatcctcctc gcccttgctc accatggtgg ctttcccggg
//
