LOCUS Exported 6461 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMXs-gw SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6461) AUTHORS Takahashi K, Yamanaka S TITLE Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. JOURNAL Cell. 2006 Aug 25. 126(4):663-76. PUBMED 16904174 REFERENCE 2 (bases 1 to 6461) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6461 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(100..204) /gene="bla" /label=AmpR promoter LTR 234..827 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 890..1247 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1306..1722 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1652..1674 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1690..1706 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1732..2106 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 2044..2060 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" protein_bind 2157..2281 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 2306..2336 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 2390..3049 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(2456..2475) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 3391..3696 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 3575..3594 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(3737..3861) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" LTR 4178..4647 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(4777..4794) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4948..5536) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5028..5047) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5700..99) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6323..6342 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 61 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cataatgaaa 241 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa 301 aatacataac tgagaataga aaagttcaga tcaaggtcag gaacagatgg aacagctgaa 361 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca 421 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc 481 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac 541 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta 601 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag 661 agcccacaac ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccgggtaccc 721 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga 781 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc 841 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag 901 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc 961 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg 1021 gaacacccgg ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc 1081 cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc ctaataggag 1141 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt 1201 ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg 1261 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc 1321 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag 1381 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt 1441 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc 1501 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg 1561 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg 1621 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct 1681 cgatcctccc tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag 1741 atcttatatg gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt 1801 actaacagcc cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc 1861 tggagacctc tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct 1921 taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc 1981 tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc 2041 gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct 2101 agactgccgg atctagctag ttaattaagg atcccagtgt ggtggtacgg gaaatcacaa 2161 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt 2221 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat 2281 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg 2341 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa 2401 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 2461 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 2521 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 2581 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 2641 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 2701 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 2761 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 2821 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 2881 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 2941 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat 3001 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc 3061 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag 3121 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt 3181 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata 3241 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct 3301 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct 3361 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga 3421 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga 3481 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt 3541 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt 3601 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc 3661 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc 3721 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg 3781 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc 3841 agctttcttg tacaaagtgg tgatattcca gctgagcgcc ggtcgctacc attaccagtt 3901 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag 3961 gaaatccatt atgtactatt taaactcgag cggccgccag cacagtggtc gacgataaaa 4021 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt 4081 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata 4141 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata 4201 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat 4261 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat 4321 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg 4381 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct 4441 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact 4501 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct 4561 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 4621 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt 4681 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg 4741 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 4801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 4861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 4921 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 4981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 5041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 5101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 5161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 5221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 5281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 5341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 5401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 5461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 5521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 5581 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 5641 ccttttgcgg ccggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 5701 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 5761 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 5821 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 5881 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 5941 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 6001 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 6061 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 6121 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 6181 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 6241 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 6301 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 6361 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 6421 agttcgatgt aacccactcg tgcacccaac tgatcttcag c //