LOCUS Exported 7644 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS c-myc promoter (Del 1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7644) AUTHORS He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B, Kinzler KW TITLE Identification of c-MYC as a target of the APC pathway. JOURNAL Science. 1998 Sep 4. 281(5382):1509-12. PUBMED 9727977 REFERENCE 2 (bases 1 to 7644) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7644 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 2908..2917 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2914..4566 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(2980..2998) /label=LucNrev /note="Firefly luciferase, reverse primer" polyA_signal 4607..4728 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4618..4637) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4672..4691 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(4976..4993) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5147..5735) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5227..5246) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5906..6766) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6529..6548 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6767..6871) /gene="bla" /label=AmpR promoter rep_origin 6898..7353 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6985..7004) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7195..7216 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 7484..7532 /note="synthetic polyadenylation signal" misc_feature 7546..7637 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 7586..7605 /label=RVprimer3 /note="pGL3 vector, forward primer" ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccgg gctcgacaga tttcacgttt gccattaccg 61 gttctccata gggtgatgtt cattagcagt ggtgataggt taattttcac catctcttat 121 gcggttgaat agtcacctct gaaccacttt ttcctccagt aactcctctt tcttcggacc 181 ttctgcagcc aacctgaaag aataacaagg aggtggctgg aaacttgttt taaggaaccg 241 cctgtccttc ccccgctgga aaccttgcac ctcggacgct cctgctcctg cccccacctg 301 acccccgccc tcgttgacat ccaggcgcga tgatctctgc tgccagtaga gggcacactt 361 actttacttt cgcaaacctg aacgcgggtg ctgcccagag agggggcgga gggaaagacg 421 ctttgcagca aaatccagca tagcgattgg ttgctccccg cgtttgcggc aaaggcctgg 481 aggcaggagt aatttgcaat ccttaaagct gaattgtgca gtgcatcgga tttggaagct 541 actatattca cttaacactt gaacgctgag ctgcaaactc aacgggtaat aacccatctt 601 gaacagcgta catgctatac acgcacccct ttcccccgaa ttgttttctc ttttggaggt 661 ggtggaggga gagaaaagtt tacttaaaat gcctttgggt gagggaccaa ggatgagaag 721 aatgtttttt gtttttcatg ccgtggaata acacaaaata aaaaatcccg agggaatata 781 cattatatat taaatataga tcatttcagg gagcaaacaa atcatgtgtg gggctgggca 841 actagctaag tcgaagcgta aataaaatgt gaatacacgt ttgcgggtta catacagtgc 901 actttcacta gtattcagaa aaaattgtga gtcagtgaac taggaaatta atgcctggaa 961 ggcagccaaa ttttaattag ctcaagactc cccccccccc aaaaaaaggc acggaagtaa 1021 tactcctctc ctcttctttg atcagaatcg atgcattttt tgtgcatgac cgcatttcca 1081 ataataaaag gggaaagagg acctggaaag gaattaaacg tccggtttgt ccggggagga 1141 aagagttaac ggtttttttc acaagggtct ctgctgactc ccccggctcg gtccacaagc 1201 tctccacttg ccccttttag gaagtccggt cccgcggttc gggtaccccc tgcccctccc 1261 atattctccc gtctagcacc tttgatttct cccaaacccg gcagcccgag actgttgcaa 1321 accggcgcca cagggcgcaa aggggatttg tctcttctga aacctggctg agaaattggg 1381 aactccgtgt gggaggcgtg ggggtgggac ggtggggtac agactggcag agagcaggca 1441 acctccctct cgccctagcc cagctctgga acaggcagac acatctcagg gctaaacaga 1501 cgcctcccgc acggggcccc acggaagcct gagcaggcgg ggcaggaggg gcggtatctg 1561 ctgctttggc agcaaattgg gggactcagt ctgggtggaa ggtatccaat ccagatagct 1621 gtgcatacat aatgcataat acatgactcc ccccaacaaa tgcaatggga gtttattcat 1681 aacgcgctct ccaagtatac gtggcaatgc gttgctgggt tattttaatc attctaggca 1741 tcgttttcct ccttatgcct ctatcattcc tccctatcta cactaacatc ccacgctctg 1801 aacgcgcgcc cattaatacc cttctttcct ccactctccc tgggactctt gatcaaagcg 1861 cggccctttc cccagcctta gcgaggcgcc ctgcagcctg gtacgcgcgt ggcgtggcgg 1921 tgggcgcgca gtgcgttctc ggtgtggagg gcagctgttc cgcctgcgat gatttatact 1981 cacaggacaa ggatgcggtt tgtcaaacag tactgctacg gaggagcagc agagaaaggg 2041 agagggtttg agagggagca aaagaaaatg gtaggcgcgc gtagttaatt catgcggctc 2101 tcttactctg tttacatcct agagctagag tgctcggctg cccggctgag tctcctcccc 2161 accttcccca ccctccccac cctccccata agcgcccctc ccgggttccc aaagcagagg 2221 gcgtggggga aaagaaaaaa gatcctctct cgctaatctc cgcccaccgg ccctttataa 2281 tgcgagggtc tggacggctg aggacccccg agctgtgctg ctcgcggccg ccaccgccgg 2341 gccccggccg tccctggctc ccctcctgcc tcgagaaggg cagggcttct cagaggcttg 2401 gcgggaaaaa gaacggaggg agggatcgcg ctgagtataa aagccggttt tcggggcttt 2461 atctaactcg ctgtagtaat tccagcgaga ggcagaggga gcgagcgggc ggccggctag 2521 ggtggaagag ccgggcgagc agagctgcgc tgcgggcgtc ctgggaaggg agatccggag 2581 cgaatagggg gcttcgcctc tggcccagcc ctcccgctga tcccccagcc agcggtccgc 2641 aacccttgcc gcatccacga aactttgccc atagcagcgg gcgggcactt tgcactggaa 2701 cttacaacac ccgagcaagg acgcgactct cccgacgcgg ggaggctatt ctgcccattt 2761 ggggacactt ccccgccgct gccaggaccc gcttctctga aaggctctcc ttgcagctgg 2821 acgtcgatat cgaattcggg tatataatgg atccggtatc gagatctgcg atctaagtaa 2881 gcttggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa cataaagaaa 2941 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct 3001 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg 3061 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga 3121 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt 3181 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat 3241 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc 3301 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt 3361 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca 3421 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac 3481 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct 3541 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat 3601 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg 3661 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa 3721 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca 3781 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta 3841 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag 3901 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt 3961 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt 4021 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa 4081 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac 4141 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac 4201 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg 4261 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc 4321 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac 4381 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg 4441 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa 4501 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc 4561 gtgtaattct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat 4621 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt 4681 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat 4741 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa 4801 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt 4861 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac 4921 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc 4981 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 5041 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 5101 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5161 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 5221 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 5281 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 5341 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 5401 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 5461 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 5521 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 5581 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 5641 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 5701 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5761 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5821 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 5881 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 5941 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6001 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6061 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6121 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6181 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6241 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6301 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6361 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6421 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6481 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6541 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6601 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6661 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6721 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6781 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6841 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 6901 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 6961 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 7021 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 7081 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 7141 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 7201 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 7261 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 7321 cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact 7381 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat 7441 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt 7501 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca 7561 tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg 7621 tgccagaaca tttctctatc gata //