LOCUS Exported 1653 bp ds-DNA linear SYN 14-JUN-2021 DEFINITION Expresses channelrhodopsin fused to mCherry for monosynaptic circuit mapping and optogenetic activation. ACCESSION . VERSION . KEYWORDS N2c(dG)-hChR2-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1653) TITLE Jessell Rabies Virus CVS-N2c DG JOURNAL Unpublished REFERENCE 2 (bases 1 to 1653) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1653 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 4..13 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 10..936 /codon_start=1 /product="H134R mutant of an N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2005)" /label=ChR2(H134R) /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGAV" CDS 949..1653 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE GRHSTGGMDELYK" primer_bind complement(1091..1109) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1359..1378 /label=mCherry-F /note="mCherry, forward primer" ORIGIN 1 aaagccacca tggactatgg cggcgctttg tctgccgtcg gacgcgaact tttgttcgtt 61 actaatcctg tggtggtgaa cgggtccgtc ctggtccctg aggatcaatg ttactgtgcc 121 ggatggattg aatctcgcgg cacgaacggc gctcagaccg cgtcaaatgt cctgcagtgg 181 cttgcagcag gattcagcat tttgctgctg atgttctatg cctaccaaac ctggaaatct 241 acatgcggct gggaggagat ctatgtgtgc gccattgaaa tggttaaggt gattctcgag 301 ttcttttttg agtttaagaa tccctctatg ctctaccttg ccacaggaca ccgggtgcag 361 tggctgcgct atgcagagtg gctgctcact tgtcctgtca tccttatccg cctgagcaac 421 ctcaccggcc tgagcaacga ctacagcagg agaaccatgg gactccttgt ctcagacatc 481 gggactatcg tgtggggggc taccagcgcc atggcaaccg gctatgttaa agtcatcttc 541 ttttgtcttg gattgtgcta tggcgcgaac acattttttc acgccgccaa agcatatatc 601 gagggttatc atactgtgcc aaagggtcgg tgccgccagg tcgtgaccgg catggcatgg 661 ctgtttttcg tgagctgggg tatgttccca attctcttca ttttggggcc cgaaggtttt 721 ggcgtcctga gcgtctatgg ctccaccgta ggtcacacga ttattgatct gatgagtaaa 781 aattgttggg ggttgttggg acactacctg cgcgtcctga tccacgagca catattgatt 841 cacggagata tccgcaaaac caccaaactg aacatcggcg gaacggagat cgaggtcgag 901 actctcgtcg aagacgaagc cgaggccgga gccgtgccag cggccgccgt gagcaagggc 961 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc 1021 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 1081 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc 1141 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc 1201 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag 1261 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac 1321 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 1381 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag 1441 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 1501 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac 1561 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc 1621 cactccaccg gcggcatgga cgagctgtac aag //