LOCUS Exported 13462 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation. ACCESSION . VERSION . KEYWORDS TetO-FUW-OSKM SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13462) AUTHORS Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, Jaenisch R TITLE Reprogramming of murine and human somatic cells using a single polycistronic vector. JOURNAL Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. PUBMED 19109433 REFERENCE 2 (bases 1 to 13462) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13462 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 128..147 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 222..326 /gene="bla" /label=AmpR promoter CDS 327..1187 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(545..564) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1358..1946 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1847..1866 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2100..2117 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2234..2255 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2270..2300 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2308..2324 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2313..2335 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2332..2348 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2332..2348 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" polyA_signal 2397..2518 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2408..2427) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2462..2481 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(2648..3022) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" promoter complement(3041..3088) /label=EM7 promoter /note="synthetic bacterial promoter " promoter complement(3136..3465) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3150..3285 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(3204..3223) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 3450..3470 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin complement(3479..3907) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3589..3610) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3801..3820 /label=F1ori-R /note="F1 origin, reverse primer" polyA_signal 3953..4177 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 4166..4183 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" LTR 4209..4389 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind 4894..4910 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 4895..4911 /label=KS primer /note="common sequencing primer, one of multiple similar variants" misc_feature 4914..5502 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS 5020..5031 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind 5429..5449 /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6909..6968) /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /label=E2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" CDS complement(8439..8492) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS complement(9471..9527) /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" primer_bind complement(10680..10704) /label=LNCX /note="Human CMV promoter, forward primer" promoter complement(10701..10739) /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" protein_bind 10772..11042 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" misc_feature 11099..11216 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" CDS complement(11514..11558) /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 11743..11976 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 12469..12594 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" LTR 12641..12821 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(12839..13037) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(12864..12884) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 13039..13418 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa 61 attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt 121 aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc 181 ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 541 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 1021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 1081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 1141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 1201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 1261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 1321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 1381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 1441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 1501 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 1561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 1621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 1681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 1741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 1801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 1861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 1921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 1981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 2101 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc 2161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 2221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 2281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 2341 gctatgacca tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag 2401 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg 2461 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg 2521 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc 2581 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta 2641 gcacgtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca 2701 gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt 2761 cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca 2821 cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca 2881 gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc 2941 cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa 3001 cggcactggt caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga 3061 tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca 3121 agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg 3181 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc 3241 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg 3301 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga 3361 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct 3421 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa 3481 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa 3541 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac 3601 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag 3661 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt 3721 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg 3781 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca 3841 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag 3901 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag 3961 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc 4021 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca 4081 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag 4141 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa 4201 acgggccctg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc 4261 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg 4321 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca 4381 gagagaccca gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct 4441 gtcaaacctc cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg 4501 gtgtaacaag cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg 4561 tactagcttg tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta 4621 gttctgccaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg 4681 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag 4741 atctacagct gccttgtaag tcattggtct taaaggtacc tgaggtgtga ctggaaaacc 4801 cacctcctcc tcctcttgtg cttctagcca ggcacaatca gcattggtag ctgctgtatt 4861 gctacttgtg attgctccat gtttttctag gtctcgaggt cgacggtatc gatgcgggga 4921 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg 4981 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta 5041 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga 5101 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca 5161 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa 5221 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc 5281 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga 5341 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg 5401 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat 5461 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc 5521 gaattcggcg cgccttatgc accagagttt cgaagctgtt cgagtttgtg tttcaactgt 5581 tctcgtcgtt tcctcaataa gtccttttca gaggtgagct tgtgctcgtc tgcttgaatg 5641 gacaggatgt aggcggtggc ttttttgagg atcactacct tgggggcctt ttcgttgttt 5701 tccaattcag ggatctggtc acgcagggca aaaaagctgc gcttcagctc gttcctcctc 5761 tgacgttcca agacgttgtg tgtccgcctc ttgtcgtttt cctccgtgtc tgaggacctg 5821 gggctggagc acttgcggtt gttgctgatc tgcttcagga ccctgccact gtccaacttg 5881 gccctcttgg cagctggata gtccttcctt gtggaggggg gtgcggcgta gttgtgctgg 5941 tgagtggaga cgtggcacct cttgaggacc agtgggctgt gcggaggttt gctgtggcct 6001 cgggatggag atgagcccga ctccgacctc ttggcagggg tttgcctctt ctccacagac 6061 accacatcaa tttcttcctc atcttcttgc tcttcttcag agtcgctgct ggtggtgggc 6121 ggtgtctcct catgcagcac taggggctca gggctggccc gtggggagga ctcggaggac 6181 agcagcgagt ccgaggaagg agagaaggcc gtggaatcgg acgaggtaca ggatttgggc 6241 gagctgctgt cgttgagcgg gtagggaaag accactgagg ggtcaatgca ctcggacgcg 6301 gcggcggtga ggtcctgcag gtacaggctg gaggtggagc agacgctgtg cccgcgggcg 6361 gggctcaggc tggtgctgtc tttgcgcgca gcctggtagg aggccagctt ctccgagacc 6421 agcttggcag cggctgagaa accgctccac atacagtcct ggatgatgat gttcttgatg 6481 aaggtctcgt cgtcaggatc gcagatgaag ctctggttca ccatgtctcc tccaagtaac 6541 tcggtcatca tctccagctg atcggcggtg gagaagttgc caccgccgcc gtcatcgtct 6601 tcccttgggg agaaggacgt agcgaccgca acataggatg gagagcagag cccggagcgg 6661 cggctcgggg acaggggcgg ggtgggaagc agctcgaatt tcttccagat atcctcactg 6721 ggcgcgggcg gctgcagctc gctctgctgt tgctggtgat agaaattctc ttcctcgtcg 6781 cagatgaaat agggctgtac ggagtcgtag tcgaggtcat agttcctgtt ggtgaagttc 6841 acgttgaggg gcatcgtcgt ggctgtctgc ggggtttcca acgcccaaag gaaatccagt 6901 ggcgcgccgg gacctgggtt gctctcaaca tctccagcca atttcaagag agcataatta 6961 gtacactggc ccgagccctc gagaaagtgc ctcttcatgt gtaaggcaag gtggtccgac 7021 ctggaaaagg ccctgtcgca cttctggcac tgaaagggcc ggtgccctgt gtgtttgcgg 7081 tagtgcctgg tcagttcatc ggagcgggcg aatttccacc cacagccgtc ccagtcacag 7141 tggtaaggtt tctcgcctgt gtgagttcgc aggtgtgcct tgagatgaga actcttggta 7201 taggttttgc cacagcctgc atagtcacaa gtgtgggtgg ctgttctttt ccggggccac 7261 gaccttcttc ccctctttgg cttgggctcc tctggcaggc aggaacccgg tggcatgagc 7321 tcttgataat ggagagaggg gacttgtgac tgcatctggt ctggcaggaa aggagggtag 7381 ttgggccccg gatggggatg gaatcctggg ggaagaggca ggccagggtg acagtccctg 7441 ctgttcagca gttcctcggg actcagtgta ggggtagtcc tggtggggag ctgccgcccc 7501 agggggaagt cgtgtgtgtt gggccggtgg ccgttgctga gctggggtcc agcgctcaaa 7561 tgggcctcta gggaccggct gaccgtgcag gacgggaccg cctcttgctt aatcttgggg 7621 cacatgcgcg gcgggccacc gctgtagggc gccactacca cggggtggct gccgtctggg 7681 cttcctttgc taacactgat gaccgaaggg ctgctgtact cgctgccagg ggtggtcaga 7741 gacgccttca gcacaaactt gcccatcagc ccgccacctg gcggctgagg ctgctgtggc 7801 ggaatgtata ctgggtccaa ctccggccgc aggagctcag ccacgaagcc gcccgagggg 7861 ctcacgtcat tgatgtccgc caggttgaag ggggccgtgg gaggtggcgc agattctcgg 7921 ctgtagagga gccctccacc tgtgttgctg gcagccacgc ccgggtcacc cccggcccgg 7981 atcggatagc tgaagctgca ggtggagggc gcgctggcag ggccgctgct cgctggggaa 8041 gacgaggatg aagctgacgc cgaggtggtc acggtggcgg ccaccgattc ctggtgggtt 8101 agcgagttgg aaaggataaa gtctaggtcc aggaggtcgt tgaactcctc ggtctccctc 8161 cgggctagga gggccgggtt gttactgctg caagctgcac cagctccgcc actctccagg 8221 tctgtggcca ccgtcgccgc caggtcgtag gggcggccgg gaagtggggg aagtcgcttc 8281 atgtgagaga gttcctcacg ccaacggtta gtcggggcac ctgctggacg cagtgtcttc 8341 tcccttcccg ccgggccgga cgcgaacgtg gagaaggacg ggagcagagc gtcgctgaca 8401 gccatgtcag actcgccagg tggctgcctc atctcgagtg ggccgggatt ttcctccacg 8461 tccccgcatg ttagaagact tcccctgccc tcgccggagc cgcatgccat gtgcgacagg 8521 ggcagtgtgc cgttaatggc cgtgccgggc accgggccgc tctggtagtg ctgggccatg 8581 tgcagtctac tgggcgcagc gggctccggc acctcggcgc cggggaggta catgctgatc 8641 atgtcccgga ggtccccggc ctggcagggc gccctggagt gggaggaaga ggtaaccacg 8701 ggggggctgg agctggcctc ggacttgacc acagagccca tggagcccag cgccataccg 8761 ggggtgccct gctgcgagta ggacatgctg taggtgggcg agccgttcat gtaggtctgc 8821 gagctggtca tggagttgta ctgcagggcg ctgacgtcgt agcggtgcat cggttgcatc 8881 tgtgccgcgc cgtgagcgtt gaggcccggg tgctgcgggt agcccagctg ctcctgcatc 8941 atgctgtagc tgccgttgct ccagccgttc atgtgcgcgt agctgtccat gcgctggttc 9001 acgcccgcac ccaggccggc gcccacccca accccgctcg ccatgctgtt cccgccgggg 9061 gccagcaagc ctccgggaag cgtgtactta tccttcttca tgagcgtctt ggttttccgc 9121 cgcggccggt atttataatc cgggtgctcc ttcatgtgca gagcgcgcag ccgcttggcc 9181 tcgtcgatga acggccgctt ctcggtctcg gacaaaagtt tccactccgc gcccaggcgc 9241 ttgctgatct ccgagttgtg catcttgggg ttctcctggg ccatcttacg ccgctgcccc 9301 cgggaccata ccatgaaggc gttcatgggc ctcttgacgc ggtccgggct gttcttctgg 9361 ttgccgccgg tcgccgccgc cgtggcgttg cctcctccgc cgccgccccc cgaagcttgc 9421 tgcgggcccg gcggcttcag ctccgtctcc atcatgttat acatgcatgc aggcccgggg 9481 ttttcttcaa catctcctgc ttgctttaac agagagaagt tcgtggcgcc gctgccgcta 9541 gcgtttgaat gcatgggaga gcccagagca gtgacgggaa cagagggaaa ggcctcgccc 9601 tcaggaaaag ggactgagta gagtgtggtg aagtgggggc ttccatagcc tggggtgcca 9661 aagtggggac ctgggggcag aggaaaggat acagcccccc ctgggaaagg tgtccctgta 9721 gcctcatact cttctcgttg ggaatactca atacttgatc ttttgccctt ctggcgccgg 9781 ttacagaacc atactcgaac cacatccttc tctagcccaa gctgattggc gatgtgagtg 9841 atctgctgta gggagggctt cgggcacttc agaaacatgg tctccagact ccacctcaca 9901 cggttctcaa tgctagttcg ctttctcttc cgggcctgca ccagggtctc cgatttgcat 9961 atctcctgaa ggttctcatt gttgtcggct tcctccaccc acttctccag caggggccgc 10021 agcttacaca tgttcttaag gctgagctgc aaggcctcga agcgacagat ggtggtctgg 10081 ctgaacacct ttccaaagag aacgcccagg gtgagcccca cgtcggcctg ggtgtacccc 10141 aaggtgatcc tcttctgctt cagcagcttg gcaaactgtt ctagctcctt ctgcagggct 10201 ttcatgtcct gggactcctc gggagttggt tccaccttct ccaacttcac ggcattgggg 10261 cggtcggcac agggctcaga ggaggttccc tctgagttgc tttccactcg tgctcctgcc 10321 tggccctcag gctgcaaagt ctccacgcca acttggggga ctaggcccag tccaacctga 10381 ggtccacagt atgccatccc tccgcagaac tcgtatgcgg gcggacatgg ggagatcccc 10441 aatacctctg agcctggtcc gattccaggc ccacctggag gcccttggaa gcttagccag 10501 gttcgaggat ccacccagcc cggctccagc cctgctgacc catcaccccc acctggtggg 10561 ggtgagaagg cgaagtctga agccaggtgt ccagccatgg cgaattcggg gccgcggagg 10621 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg 10681 atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacct 10741 cgacccgggt accgagctcg actttcactt ttctctatca ctgataggga gtggtaaact 10801 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct 10861 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg 10921 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt 10981 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg 11041 gagtggtaaa ctcgagatct aaccaaactg gatctctgct gtccctgtaa taaacccgaa 11101 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta 11161 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt 11221 ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat 11281 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg 11341 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact 11401 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc 11461 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt 11521 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct 11581 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag 11641 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca 11701 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt 11761 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt 11821 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata 11881 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt 11941 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc 12001 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact 12061 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag 12121 atcagcggcc gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc 12181 taaagcttcc ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac 12241 tgtattatat aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc 12301 cagtatttgt ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc 12361 tagctccctg cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct 12421 taaccgaatt ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca 12481 cccatctctc tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg 12541 cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc 12601 tggtttccct ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact 12661 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact 12721 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca 12781 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct 12841 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt 12901 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc 12961 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta 13021 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt 13081 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt 13141 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt 13201 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg 13261 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc 13321 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt 13381 aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac 13441 atcgcgaagc agcgcaaaac gc //