LOCUS Exported 12141 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Human CaV2.2 expression in mammalian cells. ACCESSION . VERSION . KEYWORDS huCaV2.2 (pSAD442-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12141) AUTHORS Groen JL, Andrade A, Ritz K, Jalalzadeh H, Haagmans M, Bradley TE, Jongejan A, Verbeek DS, Nurnberg P, Denome S, Hennekam RC, Lipscombe D, Baas F, Tijssen MA TITLE CACNA1B mutation is linked to unique myoclonus-dystonia syndrome. JOURNAL Hum Mol Genet. 2015 Feb 15;24(4):987-993. Epub 2014 Oct 8. PUBMED 25296916 REFERENCE 2 (bases 1 to 12141) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12141 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 899..908 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8006..8047 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 8057..8074 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(8097..8114) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8103..8327 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8373..8801 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8460..8479) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8670..8691 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8810..8830) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8815..9144 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8995..9130 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9057..9076 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9192..9239 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9258..9656 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 9814..9935 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9851..9870) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9905..9924 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9984..10000) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9984..10000) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9997..10019) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10008..10024 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10032..10062) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10077..10098 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10215..10232) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10386..10974) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10466..10485) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11145..12005) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11768..11787 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12006..12110) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 caccatggtc cgcttcgggg acgagctggg cggccgctat gggggccccg gcggcggaga 961 gcgggcccgg ggcggcgggg ccggcggggc ggggggcccg ggtcccgggg ggctgcagcc 1021 cggccagcgg gtcctctaca agcaatcgat cgcgcagcgc gcgcggacca tggcgctgta 1081 caaccccatc ccggtcaagc agaactgctt caccgtcaac cgctcgctct tcgtcttcag 1141 cgaggacaac gtcgtccgca aatacgcgaa gcgcatcacc gagtggcctc cattcgagta 1201 tatgatcctg gccaccatca tcgccaactg catcgtgctg gccctggagc agcacctccc 1261 tgatggggac aaaacgccca tgtccgagcg gctggacgac acggagccct atttcatcgg 1321 gatcttttgc ttcgaggcag ggatcaaaat catcgctctg ggctttgtct tccacaaggg 1381 ctcttacctg cggaacggct ggaacgtcat ggacttcgtg gtcgtcctca cagggatcct 1441 tgccacggct ggaactgact tcgacctgcg aacactgagg gctgtgcgtg tgctgaggcc 1501 cctgaagctg gtgtctggga ttccaagttt gcaggtggtg ctcaagtcca tcatgaaggc 1561 catggttcca ctcctgcaga ttgggctgct tctcttcttt gccatcctca tgtttgccat 1621 cattggcctg gagttctaca tgggcaagtt ccacaaggcc tgtttcccca acagcacaga 1681 tgcggagccc gtgggtgact tcccctgtgg caaggaggcc ccagcccggc tgtgcgaggg 1741 cgacactgag tgccgggagt actggccagg acccaacttt ggcatcacca actttgacaa 1801 tatcctgttt gccatcttga cggtgttcca gtgcatcacc atggagggct ggactgacat 1861 cctctataat acaaacgatg cggccggcaa cacctggaac tggctctact tcatccctct 1921 catcatcatc ggctccttct tcatgctcaa cctggtgctg ggcgtgctct cgggggagtt 1981 tgccaaggag cgagagaggg tggagaaccg ccgcgccttc ctgaagctgc gccggcagca 2041 gcagatcgag cgagagctca acgggtacct ggagtggatc ttcaaggcgg aggaagtcat 2101 gctggccgag gaggacagga atgcagagga gaagtcccct ttggacgcag tgctgaagag 2161 agcggccacc aagaagagca gaaatgacct gatccacgca gaggagggag aggaccggtt 2221 tgcagatctc tgtgctgttg gatccccctt cgcccgcgcc agcctcaaga gcgggaagac 2281 agagagctcg tcatacttcc ggaggaagga gaagatgttc cggtttttta tccggcgcat 2341 ggtgaaggct cagagcttct actgggtggt gctgtgcgtg gtggccctga acacactgtg 2401 tgtggccatg gtgcattaca accagccgcg gcggcttacc acgaccctgt attttgcaga 2461 gtttgttttc ttgggtctct tcctcacaga gatgtccctg aagatgtatg gcctggggcc 2521 cagaagctac ttccggtcct ccttcaactg cttcgacttt ggggtcatcg tggggagcgt 2581 ctttgaagtg gtctgggcgg ccatcaagcc gggaagctcc tttgggatca gtgtgctgcg 2641 ggccctccgc ctgctgagga tcttcaaagt cacgaagtac tggagctccc tgcggaacct 2701 ggtggtgtcc ctgctgaact ccatgaagtc catcatcagc ctgctcttct tgctcttcct 2761 gttcattgtg gtcttcgccc tgctggggat gcagctgttt gggggacagt tcaacttcca 2821 ggatgagact cccacaacca acttcgacac cttccctgcc gccatcctca ctgtcttcca 2881 gatcctgacg ggagaggact ggaatgcagt gatgtatcac gggatcgaat cgcaaggcgg 2941 cgtcagcaaa ggcatgttct cgtcctttta cttcattgtc ctgacactgt tcggaaacta 3001 cactctgctg aatgtctttc tggccatcgc tgtggacaac ctggccaacg cccaagagct 3061 gaccaaggat gaagaggaga tggaagaagc agccaatcag aagcttgctc tgcaaaaggc 3121 caaagaagtg gctgaagtca gccccatgtc tgccgcgaac atctccatcg ccgcttttgt 3181 aaagcaagct cgaggtactg tatctcgcag ctcatctgtc tccagcgtga actcaccgca 3241 gcagaactcg gccaaggcgc gctcggtgtg ggagcagcgg gccagccagc tacggctgca 3301 gaacctgcgg gccagctgcg aggcgctgta cagcgagatg gaccccgagg agcggctgcg 3361 cttcgccact acgcgccacc tgcggcccga catgaagacg cacctggacc ggccgctggt 3421 ggtggagctg ggccgcgacg gcgcgcgggg gcccgtggga ggcaaagccc gacctgaggc 3481 tgcggaggcc cccgagggcg tcgaccctcc gcgcaggcac caccggcacc gcgacaagga 3541 caagaccccc gcggcggggg accaggaccg agcagaggcc ccgaaggcgg agagcgggga 3601 gcccggtgcc cgggaggagc ggccgcggcc gcaccgcagc cacagcaagg aggccgcggg 3661 gcccccggag gcgcggagcg agcgcggccg aggcccaggc cccgagggcg gccggcggca 3721 ccaccggcgc ggctccccgg aggaggcggc cgagcgggag ccccgacgcc accgcgcgca 3781 ccggcaccag gatccgagca aggagtgcgc cggcgccaag ggcgagcggc gcgcgcggca 3841 ccgcggcggc ccccgagcgg ggccccggga ggcggagagc ggggaggagc cggcgcggcg 3901 gcaccgggcc cggcacaagg cgcagcctgc tcacgaggct gtggagaagg agaccacgga 3961 gaaggaggcc acggagaagg aggctgagat agtggaagcc gacaaggaaa aggagctccg 4021 gaaccaccag ccccgggagc cacactgtga cctggagacc agtgggactg tgactgtggg 4081 tcccatgcac acactgccca gcacctgtct ccagaaggtg gaggaacagc cagaggatgc 4141 agacaatcag cggaacgtca ctcgcatggg cagtcagccc ccagacccga acactattgt 4201 acatatccca gtgatgctga cgggccctct tggggaagcc acggtcgttc ccagtggtaa 4261 cgtggacctg gaaagccaag cagaggggaa gaaggaggtg gaagcggatg acgtgatgag 4321 gagcggcccc cggcctatcg tcccatacag ctccatgttc tgtttaagcc ccaccaacct 4381 gctccgccgc ttctgccact acatcgtgac catgaggtac ttcgaggtgg tcattctcgt 4441 ggtcatcgcc ttgagcagca tcgccctggc tgctgaggac ccagtgcgca cagactcgcc 4501 caggaacaac gctctgaaat acctggatta cattttcact ggtgtcttta cctttgagat 4561 ggtgataaag atgatcgact tgggactgct gcttcaccct ggagcctatt tccgggactt 4621 gtggaacatt ctggacttca ttgtggtcag cggcgccctg gtggcgtttg ctttctcagg 4681 atccaaaggg aaagacatca ataccatcaa gtctctgaga gtccttcgtg tcctgcggcc 4741 cctcaagacc atcaaacggc tgcccaagct caaggctgtg tttgattgtg tggtgaactc 4801 cctgaagaat gtcctcaaca tcttgattgt ctacatgctc ttcatgttca tatttgccgt 4861 cattgcggtg cagctcttca aagggaagtt tttctactgc acagatgaat ccaaggagct 4921 ggagagggac tgcaggggtc agtatttgga ttatgagaag gaggaagtgg aagctcagcc 4981 caggcagtgg aagaaatacg actttcacta cgacaatgtg ctctgggctc tgctgacgct 5041 gttcacagtg tccacgggag aaggctggcc catggtgctg aaacactccg tggatgccac 5101 ctatgaggag cagggtccaa gccctgggta ccgcatggag ctgtccatct tctacgtggt 5161 ctactttgtg gtctttccct tcttcttcgt caacatcttt gtggctttga tcatcatcac 5221 cttccaggag cagggggaca aggtgatgtc tgaatgcagc ctggagaaga acgagagggc 5281 ttgcattgac ttcgccatca gcgccaaacc cctgacacgg tacatgcccc aaaaccggca 5341 gtcgttccag tataagacgt ggacatttgt ggtctccccg ccctttgaat acttcatcat 5401 ggccatgata gccctcaaca ctgtggtgct gatgatgaag ttctatgatg caccctatga 5461 gtacgagctg atgctgaaat gcctgaacat cgtgttcaca tccatgttct ccatggaatg 5521 cgtgctgaag atcatcgcct ttggggtgct gaactatttc agagatgcct ggaatgtctt 5581 tgactttgtc actgtgttgg gaagtattac tgatatttta gtaacagaga ttgcggaaac 5641 gaacaatttc atcaacctca gcttcctccg cctctttcga gctgcgcggc tgatcaagct 5701 gctccgccag ggctacacca tccgcatcct gctgtggacc tttgtccagt ccttcaaggc 5761 cctgccctac gtgtgtctgc tcattgccat gctgttcttc atctacgcca tcatcggcat 5821 gcaggtgttt gggaatattg ccctggatga tgacaccagc atcaaccgcc acaacaactt 5881 ccggacgttt ttgcaagccc tgatgctgct gttcaggagc gccacggggg aggcctggca 5941 cgagatcatg ctgtcctgcc tgagcaacca ggcctgtgat gagcaggcca atgccaccga 6001 gtgtggaagt gattttgcct acttctactt cgtctccttc atcttcctgt gctcctttct 6061 gatgttgaac ctctttgtgg ctgtgatcat ggacaatttt gagtacctca cgcgggactc 6121 ttccatccta ggtcctcacc acttggatga gttcatccgg gtctgggctg aatacgaccc 6181 ggctgcgtgt gggcgcatca gttacaatga catgtttgag atgctgaaac acatgtcccc 6241 gcctctgggg ctggggaaga aatgccctgc tcgagttgct tacaagcgcc tggttcgcat 6301 gaacatgccc atctccaacg aggacatgac tgttcacttc acgtccacgc tgatggccct 6361 catccggacg gcactggaga tcaagctggc cccagctggg acaaagcagc atcagtgtga 6421 cgcggagttg aggaaggaga tttccgttgt gtgggccaat ctgccccaga agactttgga 6481 cttgctggta ccaccccata agcctgatga gatgacagtg gggaaggttt atgcagctct 6541 gatgatattc gacttctaca agcagaacaa aaccaccaga gaccagatgc agcaggctcc 6601 tggaggcctc tcccagatgg gtcctgtgtc cctgttccac cctctgaagg ccaccctgga 6661 gcagacacag ccggctgtgc tccgaggagc ccgggttttc cttcgacaga agagttccac 6721 ctccctcagc aatggcgggg ccatacaaaa ccaagagagt ggcatcaaag agtctgtctc 6781 ctggggcact caaaggaccc aggatgcacc ccatgaggcc aggccacccc tggagcgtgg 6841 ccactccaca gagatccctg tggggcggtc aggagcactg gctgtggacg ttcagatgca 6901 gagcataacc cggaggggcc ctgatgggga gccccagcct gggctggaga gccagggtcg 6961 agcggcctcc atgccccgcc ttgcggccga gactcagccc gtcacagatg ccagccccat 7021 gaagcgctcc atctccacgc tggcccagcg gccccgtggg actcatcttt gcagcaccac 7081 cccggaccgc ccacccccta gccaggcgtc gtcgcaccac caccaccacc gctgccaccg 7141 ccgcagggac aggaagcaga ggtccctgga gaaggggccc agcctgtctg ccgatatgga 7201 tggcgcacca agcagtgctg tggggccggg gctgcccccg ggagaggggc ctacaggctg 7261 ccggcgggaa cgagagcgcc ggcaggagcg gggccggtcc caggagcgga ggcagccctc 7321 atcctcctcc tcggagaagc agcgcttcta ctcctgcgac cgctttgggg gccgtgagcc 7381 cccgaagccc aagccctccc tcagcagcca cccaacgtcg ccaacagctg gccaggagcc 7441 gggaccccac ccacagggca gtggttccgt gaatgggagc cccttgctgt caacatctgg 7501 tgctagcacc cccggccgcg gtgggcggag gcagctcccc cagacgcccc tgactccccg 7561 ccccagcatc acctacaaga cggccaactc ctcacccatc cacttcgccg gggctcagac 7621 cagcctccct gccttctccc caggccggct cagccgtggg ctttccgaac acaacgccct 7681 gctgcagaga gaccccctca gccagcccct ggcccctggc tctcgaattg gctctgaccc 7741 ttacctgggg cagcgtctgg acagtgaggc ctctgtccac gccctgcctg aggacacgct 7801 cactttcgag gaggctgtgg ccaccaactc gggccgctcc tccaggactt cctacgtgtc 7861 ctccctgacc tcccagtctc accctctccg ccgcgtgccc aacggttacc actgcaccct 7921 gggactcagc tcgggtggcc gagcacggca cagctaccac caccctgacc aagaccactg 7981 gtgctagtct agagggccct tcgaaggtaa gcctatccct aaccctctcc tcggtctcga 8041 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc 8101 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 8161 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 8221 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 8281 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 8341 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc 8401 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 8461 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 8521 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 8581 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 8641 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 8701 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 8761 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt 8821 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 8881 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 8941 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 9001 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 9061 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 9121 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 9181 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca 9241 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa 9301 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag 9361 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac 9421 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt 9481 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac 9541 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc 9601 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac 9661 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc 9721 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 9781 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 9841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 9901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 9961 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 10021 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 10081 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 10141 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 10201 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 10261 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 10321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 10381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 10441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 10501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 10561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 10621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 10681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 10741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 10801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 10861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 10921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 10981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 11041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 11101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 11161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 11221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 11281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 11341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 11401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 11461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 11521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 11581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 11641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 11701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 11761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 11821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 11881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 11941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 12001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 12061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 12121 cgaaaagtgc cacctgacgt c //