LOCUS Exported 9194 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pQCXI Neo DsRed-LC3-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9194) AUTHORS Sheen JH, Zoncu R, Kim D, Sabatini DM TITLE Defective Regulation of Autophagy upon Leucine Deprivation Reveals a Targetable Liability of Human Melanoma Cells In Vitro and In Vivo. JOURNAL Cancer Cell. 2011 May 17. 19(5):613-28. PUBMED 21575862 REFERENCE 2 (bases 1 to 9194) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9194 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 309..512 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 513..688 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 751..950 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1151..1567 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1497..1519 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1535..1551 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" enhancer 1604..1907 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1908..2111 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2108..2132 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 2253..2262 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2259..2933 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Strongin et al., 2007)" /label=DsRed-Monomer /note="mammalian codon-optimized" /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS GSQ" primer_bind complement(2439..2459) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 2657..2676 /label=mCherry-F /note="mCherry, forward primer" CDS 3366..4079 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 3366..4079 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(3408..3429) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3669..3688) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4016..4037 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4103..4675 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4270..4287) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4497..4516 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4689..5483 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4743..4762) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5353..5372 /label=Neo-F /note="Neomycin resistance gene, forward primer" LTR 5900..6325 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus" primer_bind complement(6428..6450) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6586..6606) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6591..6920 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6771..6906 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6833..6852 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 6942..6961 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(7042..7059) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7213..7801) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7293..7312) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7972..8832) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8595..8614 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8833..8937) /gene="bla" /label=AmpR promoter primer_bind 9005..9023 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" enhancer 9123..308 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1921 ggcagtacat caatgggcgt gaatagcggt ttgactcacg gggatttcca agtctccacc 1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 2221 cggacctgca ggaattgatc cgcggccgct gagccaccat ggacaacacc gaggacgtca 2281 tcaaggagtt catgcagttc aaggtgcgca tggagggctc cgtgaacggc cactacttcg 2341 agatcgaggg cgagggcgag ggcaagccct acgagggcac ccagaccgcc aagctgcagg 2401 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg 2461 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacatgaag ctgtccttcc 2521 ccgagggctt cacctgggag cgctccatga acttcgagga cggcggcgtg gtggaggtgc 2581 agcaggactc ctccctgcag gacggcacct tcatctacaa ggtgaagttc aagggcgtga 2641 acttccccgc cgacggcccc gtaatgcaga agaagactgc cggctgggag ccctccaccg 2701 agaagctgta cccccaggac ggcgtgctga agggcgagat ctcccacgcc ctgaagctga 2761 aggacggcgg ccactacacc tgcgacttca agaccgtgta caaggccaag aagcccgtgc 2821 agctgcccgg caaccactac gtggactcca agctggacat caccaaccac aacgaggact 2881 acaccgtggt ggagcagtac gagcacgccg aggcccgcca ctccggctcc cagtccggac 2941 tcagatctcg agctcaagct tcgaattctg gtggtggtcc gtccgagaag accttcaaac 3001 agcgccggag cttcgaacaa agagtggaag atgtccggct catccgggag cagcacccca 3061 ccaagatccc agtgattata gagcgataca agggtgagaa gcagctgccc gtcctggaca 3121 agaccaagtt ccttgtacct gatcacgtga atatgagcga actcatcaag ataattagaa 3181 ggcgcctgca gctcaatgct aaccaagcct tcttcctcct ggtgaatggg cacagcatgg 3241 tgagtgtgtc cacacccatc tctgaagtgt acgagagcga gagagatgaa gacggcttcc 3301 tgtacatggt ctatgcctcc caggagacgt tcgggacagc actggctgtg gtcgacggtg 3361 gtggtgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 3421 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 3481 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 3541 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 3601 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 3661 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 3721 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 3781 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 3841 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 3901 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 3961 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 4021 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga 4081 agctttagga tccggaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa 4141 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 4201 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 4261 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 4321 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 4381 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 4441 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 4501 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 4561 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg 4621 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg 4681 ccacaaccat ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 4741 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 4801 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 4861 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 4921 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 4981 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 5041 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 5101 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 5161 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca 5221 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 5281 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 5341 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 5401 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 5461 gccttcttga cgagttcttc tgagcgggat cggctagagt cgacccggac gggacgctcc 5521 tgcgcctgat acagaacgaa ttgcttgcag gcatctcatg agtgtgtctt cccgttttcc 5581 gcctgaggtc actgcgtgga tggagcgctg gcgcctgctg cgcgacggcg agctgctcac 5641 cacccactcg agggctgcag cgctgcagag gccgagtgca gaactgctcc aaagggacct 5701 caaggctttc cgagggacac taggctgact ccatcgagcc agtgtagaga taagcttatc 5761 gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac 5821 aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag attttattta 5881 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag 5941 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa 6001 ggtcaggaac agatggaaca gggtcgaccc tagagaacca tcagatgttt ccagggtgcc 6061 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct 6121 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg 6181 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg 6241 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac 6301 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca 6361 gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc ggtgatgacg 6421 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 6481 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 6541 ccatgaccca gtcacgtagc gatagcggag tgtagatccg gctgtggaat gtgtgtcagt 6601 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 6661 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 6721 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 6781 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 6841 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 6901 gaggcctagg cttttgcaaa aagcttactg gcttaactat gcggcatcag agcagattgt 6961 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 7021 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 7081 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7141 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7201 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7261 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7321 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7381 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7441 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7501 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7561 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7621 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 7681 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7741 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7801 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7861 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7921 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7981 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 8041 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 8101 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8161 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8221 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8281 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8341 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8401 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8461 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8521 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8581 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8641 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8701 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 8761 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 8821 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8881 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8941 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 9001 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttagcttggc cattgcatac 9061 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg 9121 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 9181 cccatatatg gagt //