LOCUS Exported 755 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.. ACCESSION . VERSION . KEYWORDS lentiGuide-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 755) AUTHORS Sanjana NE, Shalem O, Zhang F TITLE Improved vectors and genome-wide libraries for CRISPR screening. JOURNAL Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PUBMED 25075903 REFERENCE 2 (bases 1 to 755) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..755 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 6..246 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 6..26 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 177..196 /label=LKO.1 5' /note="Human U6 promoter, forward primer" ORIGIN 1 ccagagaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta 61 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac 121 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact 181 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga 241 aaggacgaaa caccggagac ggttgtaaat gagcacacaa aatacacatg ctaaaatatt 301 atattctatg acctttataa aatcaaccaa aatcttcttt ttaataactt tagtatcaat 361 aattagaatt tttatgttcc tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa 421 cgctagtttt agtaactcgc gttgttttct tcacctttaa taatagctac tccaccactt 481 gttcctaagc ggtcagctcc tgcttcaatc attttttgag catcttcaaa tgttctaact 541 ccaccagctg ctttaactaa agcattgtct ttaacaactg acttcattag tttaacatct 601 tcaaatgttg cacctgattt tgaaaatcct gttgatgttt taacaaattc taatccagct 661 tcaacagcta tttcacaagc tttcatgatt tcttcttttg ttaataaaca attttccata 721 atacatttaa caacatgtga tccagctgct ttttt //