LOCUS       Exported                 840 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Mammalian expression of tandem fusion of mClover3-mRuby3.
ACCESSION   .
VERSION     .
KEYWORDS    pKanCMV-mClover3-mRuby3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(20..727)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     primer_bind     complement(782..803)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatagaata gggcccttac ttgtacagct cgtccatgcc accaccaagg ttgctgtatt
       61 tggcaactgc cacttctctt tgcactacgt aggtttcatt gtcactctcc tcgatccttt
      121 ccaggcggtg atcaacggca tggacaccgg gcatcttgat gttcccgacg gtcttttttg
      181 acctgtaagt tgtcacgaag ttgcagtgca gatggccacc accatcaact ttcagtgcga
      241 tgtcagtgta tcctctcaga ccaccatctg ctggatacat catctctgta ttaggctccc
      301 aacccttggt cttcttctgc atcacgggac cattggaggg aaagtttacc cctctgacct
      361 tgacgttgta gacgagctcg ccatcctcaa ggctggtgtc ctgcgtgacg gtgacgactc
      421 caccatcttc gtatctcgta actctttccc aagtaaaacc ctcaggaaag gactgtttaa
      481 agaaatcagg gatgtcggcc gggtacttga taaaggtacg gctgccatac atgaacgacg
      541 tggcaagaat gtcaaaggca aatggcaggg gtcctccctc gatgactttg atcctcatgg
      601 tttgcactcc ctcgtacggt ctgccttctc cttcacctgt gcatttgaat tggtggccgt
      661 tgaccgaacc ttccatgacc accttcatac gcatattttc cttgatcagc tcttcgccct
      721 tagacacttc ttcgcctttg ctcaccatcg gatcttcgcc gccgctttcc agggcggcgg
      781 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg
//
