LOCUS Exported 2201 bp ds-DNA linear SYN 22-JUN-2021 DEFINITION Mammalian expression of N-terminal fusion of mClover3 to Tubulin. ACCESSION . VERSION . KEYWORDS pKanCMV-mClover3-18aa-Tubulin SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2201) AUTHORS Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J TITLE Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. JOURNAL Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. PUBMED 26879144 REFERENCE 2 (bases 1 to 2201) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 22, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2201 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 22..31 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(73..94) /label=EGFP-N /note="EGFP, reverse primer" primer_bind 681..702 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gatccgctag cgctaccggt cgccaccatg gtgtctaagg gcgaggagct gttcaccggg 61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc 121 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc 181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc 241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 301 ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc 361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccactacgtc 481 tatatcacgg ccgacaagca gaagaactgc atcaaggcta acttcaagat ccgccacaac 541 gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 601 ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac 661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggattaca 721 catggcatgg acgagctgta caagtccgga ctcagatctg gcagcggtgg aggcagcgca 781 tccggcggaa gcggaagcgt gcgtgagtgc atctccatcc acgttggcca ggctggtgtc 841 cagattggca atgcctgctg ggagctctac tgcctggaac acggcatcca gcccgatggc 901 cagatgccaa gtgacaagac cattggggga ggagatgact ccttcaacac cttcttcagt 961 gagacgggcg ctggcaagca cgtgccccgg gctgtgtttg tagacttgga acccacagtc 1021 attgatgaag ttcgcactgg cacctaccgc cagctcttcc accctgagca gctcatcaca 1081 ggcaaggaag atgctgccaa taactatgcc cgagggcact acaccattgg caaggagatc 1141 attgaccttg tgttggaccg aattcgcaag ctggctgacc agtgcaccgg tcttcagggc 1201 ttcttggttt tccacagctt tggtggggga actggttctg ggttcacctc cctgctcatg 1261 gaacgtctct cagttgatta tggcaagaag tccaagctgg agttctccat ttacccagca 1321 ccccaggttt ccacagctgt agttgagccc tacaactcca tcctcaccac ccacaccacc 1381 ctggagcact ctgattgtgc cttcatggta gacaatgagg ccatctatga catctgtcgt 1441 agaaacctcg atatcgagcg cccaacctac actaacctta accgccttat tagccagatt 1501 gtgtcctcca tcactgcttc cctgagattt gatggagccc tgaatgttga cctgacagaa 1561 ttccagacca acctggtgcc ctacccccgc atccacttcc ctctggccac atatgcccct 1621 gtcatctctg ctgagaaagc ctaccatgaa cagctttctg tagcagagat caccaatgct 1681 tgctttgagc cagccaacca gatggtgaaa tgtgaccctc gccatggtaa atacatggct 1741 tgctgcctgt tgtaccgtgg tgacgtggtt cccaaagatg tcaatgctgc cattgccacc 1801 atcaaaacca agcgcagcat ccagtttgtg gattggtgcc ccactggctt caaggttggc 1861 atcaactacc agcctcccac tgtggtgcct ggtggagacc tggccaaggt acagagagct 1921 gtgtgcatgc tgagcaacac cacagccatt gctgaggcct gggctcgcct ggaccacaag 1981 tttgacctga tgtatgccaa gcgtgccttt gttcactggt acgtgggtga ggggatggag 2041 gaaggcgagt tttcagaggc ccgtgaagat atggctgccc ttgagaagga ttatgaggag 2101 gttggtgtgg attctgttga aggagagggt gaggaagaag gagaggaata ctaaggatcc 2161 accggatcta gataactgat cataatcagc cataccacat t //