LOCUS       Exported                1022 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  lentiviral vector expressing sgRNA targeting LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    pLCKO_LacZ_sgRNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod 
            G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, 
            Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
  TITLE     High-Resolution CRISPR Screens Reveal Fitness Genes and 
            Genotype-Specific Cancer Liabilities.
  JOURNAL   Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. 
            Epub 2015 Nov 25.
  PUBMED    26627737
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(78..677)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(161..181)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     658..677
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(762..780)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
       61 ttgtcgactt aacgcgttca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct
      121 tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg
      181 cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg
      241 gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg
      301 gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc
      361 tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc
      421 gcccccgctt cgacgctctc cggcgtggtc cagaccgcca cggcggcgcc gtcgtccgcg
      481 acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg
      541 acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac
      601 gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg
      661 ggcttgtact cggtcatggt aagctccggt ggatccccct ggggagagag gtcggtgatt
      721 cggtcaacga gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac
      781 cgcgcgggca ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct
      841 tcccggccgc tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc
      901 ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt
      961 gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg
     1021 gg
//
