LOCUS       Exported                 839 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Mammalian expression of N-terminal fusion of mRuby3 to H2B..
ACCESSION   .
VERSION     .
KEYWORDS    pKanCMV-mRuby3-10aa-H2B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      34..43
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             40..750
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgtctaa gggcgaagag
       61 ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa
      121 ttcaaatgca caggtgaagg agaaggcaga ccgtacgagg gagtgcaaac catgaggatc
      181 aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg
      241 tatggcagcc gtacctttat caagtacccg gccgacatcc ctgatttctt taaacagtcc
      301 tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc
      361 gtcacgcagg acaccagcct tgaggatggc gagctcgtct acaacgtcaa ggtcagaggg
      421 gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat
      481 acagagatga tgtatccagc agatggtggt ctgagaggat acactgacat cgcactgaaa
      541 gttgatggtg gtggccatct gcactgcaac ttcgtgacaa cttacaggtc aaaaaagacc
      601 gtcgggaaca tcaagatgcc cggtgtccat gccgttgatc accgcctgga aaggatcgag
      661 gagagtgaca atgaaaccta cgtagtgcaa agagaagtgg cagttgccaa atacagcaac
      721 cttggtggtg gcatggacga gctgtacaag tccggactca gatctggagg ctccggaggc
      781 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaa
//
