LOCUS Exported 11906 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Combined sgRNA/Cas9 vector with LEU2 marker. ACCESSION . VERSION . KEYWORDS pMZ377 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11906) TITLE sgRNA/Cas9 JOURNAL Unpublished REFERENCE 2 (bases 1 to 11906) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11906 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin 539..1320 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" primer_bind 1429..1445 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 1696..2103 /gene="S. cerevisiae LEU2" /label=LEU2 promoter CDS 2104..3198 /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" primer_bind 3870..3886 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_RNA complement(3936..3987) /label=sTRSV HHRz /note="hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)" promoter complement(4106..4462) /label=rrk1 promoter/leader /note="promoter and leader RNA for the Schizosaccharomyces pombe rrk1 gene encoding the K-RNA component of RNase P (Jacobs et al., 2014)" primer_bind complement(4499..4515) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4499..4515) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4512..4534) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4523..4539 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4547..4577) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4592..4613 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4730..4747) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4901..5489) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4981..5000) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5660..6520) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6283..6302 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6521..6625) /gene="bla" /label=AmpR promoter promoter 6654..7395 /label=adh1 promoter /note="promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase" CDS 7416..11519 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11532..11552 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 11585..11832 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(11601..11619) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" primer_bind 11879..11897 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataatcg atggccaacc ttccaattca ttaaatcgag tcctccatca gatgatttac 241 aaggttttgc atagaatccc taagtaagag aagttgttag tgatttcttc aagtttacta 301 aaatttacat acaaatggat gatctcttct ccattgtttc ctttcttctt gcaaacgcgt 361 tttacaaaga gatgacatgc tattttctga ttattttttt ctatagtttt ctattttgta 421 cgtccttggg agtccgaaat gtaaaatcgg ggtatggtta gtagcggcag cgttcattat 481 ggtatataaa agataaaaag taggggcaag cgagacagaa tgggatacaa gggcatcgtc 541 tataattata gctaaaaatt gtattttaat ttgtattttt tgtaatttta tttctcattg 601 ttttacttaa aatgaatgcg aattagagaa aacttcaacg aaatgtcaaa ataagctcag 661 caaaatatac aattttaggg aaagcgatca gcaacttatt ctccgttgta gattcttttt 721 cttcataaat tccaacaatg taactgccaa ttgatttttc tctagatgct aataaattga 781 aactaatgaa tataataaca caggttttga taaattgtaa actcttactc aaaaaaagaa 841 tgttaaaact aatttaacct ttttataaag gctcatggcg ttaaagagga tagtaaaaat 901 tctacaattt tttaatttaa tgtcacaatt gtcttgcgaa tataattcta ttaattttcg 961 ctaactgctg ttttagtact attactttca atatgcctta tatgcaactt taattcatga 1021 agaatgcaat atttttgcaa tctttcttgt actgagcgct ttttatcatt tagtactcat 1081 tatttaattt ttcagtaaag ggcaataaat aaatttgtag aagatgcaat gtaatctcct 1141 ctatcctttt gctcatatgt ttatgagtat acctagtcta gaaaggcttg tatttaaaat 1201 attattcaat aaaaattcac aatttttacg acatgtgcta ccattcactt aacttcctga 1261 ttataaaatt ggttcgttta tactaattac ttaagtacct ttaactaaac aaaatgccta 1321 tatatatatt aatttacaat gagtgtcaga taagtcacta tgtccgagtg gttaaggagt 1381 tagactcgcc atcgtgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 1441 ccagtgccaa gcttgtcgag gagaacttct agtatatcta catacctaat attattgcct 1501 tattaaaaat ggaatcccaa caattacatc aaaatccaca ttctcttcaa aatcaattgt 1561 cctgtacttc cttgttcatg tgtgttcaaa aacgttatat ttataggata attatactct 1621 atttctcaac aagtaattgg ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat 1681 atcttgaccg cagttaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc 1741 tcttagcaac cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac 1801 agaatcaaat tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata 1861 cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga gagccgcgga accggctttt 1921 catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca 1981 atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta 2041 acttttctta ccttttacat ttcagcaata tatatatata tatttcaagg atataccatt 2101 ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc 2161 acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc 2221 gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt tccacttcca 2281 gatgaggcgc tggaagcctc caagaaggtt gatgccgttt tgttaggtgc tgtgggtggt 2341 cctaaatggg gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa 2401 cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta 2461 tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgtcag agaattagtg 2521 ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt 2581 gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta 2641 caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt ggcctcttca 2701 agattatgga gaaaaactgt ggaggaaacc atcaagaacg aattccctac attgaaggtt 2761 caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat 2821 ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc 2881 ccaggttcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc 2941 gcatttggtt tgtacgaacc atgccacggt tctgctccag atttgccaaa gaataaggtc 3001 aaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct 3061 gaagaaggta aggccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact 3121 ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt 3181 aagaaaatcc ttgcttaaaa agattctctt tttttatgat atttgtacat aaactttata 3241 aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga 3301 cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga ggatgtaaag 3361 gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa gaattgcact 3421 ttaacattaa tattgacaag gaggagggca ccacacaaaa agttaggtgt aacagaaaat 3481 catgaaacta tgattcctaa tttatatatt ggaggatttt ctctaaaaaa aaaaaaatac 3541 aacaaataaa aaacactcaa tgacctgacc atttgatgga gtttaagtca ataccttctt 3601 gaaccatttc ccataatggt gaaagttccc tcaagaattt tactctgtca gaaacggcct 3661 taacgacgta gtcgatcgac ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 3721 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 3781 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 3841 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg 3901 cctgcaggtc gactctagag gatccccacg gtacccctgt ttcgtcctca cggactcatc 3961 agaccggaaa gcacatccgg tgacagggca ccaccgactc ggtgccactc acttttttca 4021 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa cagagagata 4081 acccacaaga attgagttaa gcccattctt cggtacaggt tatgtttttt ggcaacagca 4141 actattcaaa tcaactgaaa gttttttaga attcaacaac ttccaatact caagtgtcca 4201 agctagcaaa ctgtttaata atcgtgaccc aacattcctt tcatgcgcac cctcagcgtc 4261 acataaatca acactttaca acacctattc aaagcttcta tatgccttcg tcatttaaat 4321 gtctaacatt tcaaaaaagt gtttgctaat ggtaaattct tgagttttct tggaagcaca 4381 tatataacga gtgtgactgt tctacgctcc ttgctgacta gtcatcagtc tacgcatgcc 4441 aactaccacc aacataagca aaaggtaccc ggggtaccga gctcgaattc gtaatcatgt 4501 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 4561 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 4621 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 4681 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 4741 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 4801 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 4861 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 4921 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 4981 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 5041 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 5101 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 5161 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 5221 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 5281 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 5341 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 5401 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 5461 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 5521 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 5581 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 5641 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 5701 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 5761 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 5821 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 5881 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 5941 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 6001 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 6061 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 6121 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 6181 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 6241 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 6301 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 6361 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 6421 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 6481 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 6541 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 6601 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgccctac 6661 aacaactaag aaaatggcta tcatgcggaa gctggtgaga agaacagcat cgggacaagg 6721 gaaggaagaa caaagacaaa gaaaacaaaa gaaagcaatt gaaaacaaaa caaaacaatt 6781 ttcattcctt ctcttatcat tccttttctt ttcttttctc tcattcaacg cactccatcg 6841 tatccgtatt cctcttattt tttctctttc tctatatcca tttctttctc tctaggtgtg 6901 tcctctctct ctcttcaatt tctctactcc gcattccaac gcatccttcc cccaacctcc 6961 catttcctcc ttacggcccg atagcgatcg tctttccctc gctatcactc gctaccggcc 7021 cctcctctgc accgtaacct cctacgtatt taccatatca taaagttttt tccgacgctt 7081 atcgctgacc ccctgtcgcc ctcctattgg cttccggatt atcttcttgt ccataaggtg 7141 atccatgctt cctgaagatt cccgaaatgt gtccactttg gcggggaatc attccatcca 7201 cttctttctc tctcgctttc ctcattcggc gctccccttc cgcgtctcat tggtcttccg 7261 ctccgttttt gctttgccga tgttacttgg ggagaggtgc gataatcctt tcgcaaaaac 7321 tcggtttgac gcctcccatg gtataaatag tgggtggtgg acaggtgcct tcgcttttct 7381 ttaagcaaga gaattgctgc aggtcgactc tagagatgga caagaagtac tccattgggc 7441 tcgatatcgg cacaaacagc gtcggttggg ccgtcattac ggacgagtac aaggtgccga 7501 gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg 7561 gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa agaacagcac 7621 ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg 7681 agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg 7741 aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc 7801 atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt actgataagg 7861 ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc 7921 tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg 7981 ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga gttgacgcca 8041 aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc atcgcacagc 8101 tccctgggga gaagaagaac ggcctgtttg gtaatcttat cgccctgtca ctcgggctga 8161 cccccaactt taaatctaac ttcgacctgg ccgaagatgc caagcttcaa ctgagcaaag 8221 acacctacga tgatgatctc gacaatctgc tggcccagat cggcgaccag tacgcagacc 8281 tttttttggc ggcaaagaac ctgtcagacg ccattctgct gagtgatatt ctgcgagtga 8341 acacggagat caccaaagct ccgctgagcg ctagtatgat caagcgctat gatgagcacc 8401 accaagactt gactttgctg aaggcccttg tcagacagca actgcctgag aagtacaagg 8461 aaattttctt cgatcagtct aaaaatggct acgccggata cattgacggc ggagcaagcc 8521 aggaggaatt ttacaaattt attaagccca tcttggaaaa aatggacggc accgaggagc 8581 tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca gcgcactttc gacaatggaa 8641 gcatccccca ccagattcac ctgggcgaac tgcacgctat cctcaggcgg caagaggatt 8701 tctacccctt tttgaaagat aacagggaaa agattgagaa aatcctcaca tttcggatac 8761 cctactatgt aggccccctc gcccggggaa attccagatt cgcgtggatg actcgcaaat 8821 cagaagagac catcactccc tggaacttcg aggaagtcgt ggataagggg gcctctgccc 8881 agtccttcat cgaaaggatg actaactttg ataaaaatct gcctaacgaa aaggtgcttc 8941 ctaaacactc tctgctgtac gagtacttca cagtttataa cgagctcacc aaggtcaaat 9001 acgtcacaga agggatgaga aagccagcat tcctgtctgg agagcagaag aaagctatcg 9061 tggacctcct cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa gaagactatt 9121 tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg agtggaggat cgcttcaacg 9181 catccctggg aacgtatcac gatctcctga aaatcattaa agacaaggac ttcctggaca 9241 atgaggagaa cgaggacatt cttgaggaca ttgtcctcac ccttacgttg tttgaagata 9301 gggagatgat tgaagaacgc ttgaaaactt acgctcatct cttcgacgac aaagtcatga 9361 aacagctcaa gaggcgccga tatacaggat gggggcggct gtcaagaaaa ctgatcaatg 9421 ggatccgaga taagcagtct ggaaagacaa tcctggattt tcttaagtcc gatggatttg 9481 ccaaccggaa cttcatgcag ttgatccatg atgactctct cacctttaag gaggacatcc 9541 agaaagcaca agtttctggc cagggggaca gtcttcacga gcacatcgct aatcttgcag 9601 gtagcccagc tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat gaactcgtca 9661 aagtaatggg aaggcataag cccgagaata tcgttatcga gatggcccga gagaaccaaa 9721 ctacccagaa gggacagaag aacagtaggg aaaggatgaa gaggattgaa gagggtataa 9781 aagaactggg gtcccaaatc cttaaggaac acccagttga aaacacccag cttcagaatg 9841 agaagctcta cctgtactac ctgcagaacg gcagggacat gtacgtggat caggaactgg 9901 acatcaatcg gctctccgac tacgacgtgg atcatatcgt gccccagtct tttctcaaag 9961 atgattctat tgataataaa gtgttgacaa gatccgataa aaatagaggg aagagtgata 10021 acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag ctgctgaacg 10081 ccaaactgat cacacaacgg aagttcgata atctgactaa ggctgaacga ggtggcctgt 10141 ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc cagatcacca 10201 agcacgtggc ccaaattctc gattcacgca tgaacaccaa gtacgatgaa aatgacaaac 10261 tgattcgaga ggtgaaagtt attactctga agtctaagct ggtctcagat ttcagaaagg 10321 actttcagtt ttataaggtg agagagatca acaattacca ccatgcgcat gatgcctacc 10381 tgaatgcagt ggtaggcact gcacttatca aaaaatatcc caagcttgaa tctgaatttg 10441 tttacggaga ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct gagcaggaaa 10501 taggcaaggc caccgctaag tacttctttt acagcaatat tatgaatttt ttcaagaccg 10561 agattacact ggccaatgga gagattcgga agcgaccact tatcgaaaca aacggagaaa 10621 caggagaaat cgtgtgggac aagggtaggg atttcgcgac agtccggaag gtcctgtcca 10681 tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac cggaggcttc tccaaggaaa 10741 gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat tgggacccca 10801 agaaatacgg cggattcgat tctcctacag tcgcttacag tgtactggtt gtggccaaag 10861 tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc atcacaatca 10921 tggagcgatc aagcttcgaa aaaaacccca tcgactttct cgaggcgaaa ggatataaag 10981 aggtcaaaaa agacctcatc attaagcttc ccaagtactc tctctttgag cttgaaaacg 11041 gccggaaacg aatgctcgct agtgcgggcg agctgcagaa aggtaacgag ctggcactgc 11101 cctctaaata cgttaatttc ttgtatctgg ccagccacta tgaaaagctc aaagggtctc 11161 ccgaagataa tgagcagaag cagctgttcg tggaacaaca caaacactac cttgatgaga 11221 tcatcgagca aataagcgaa ttctccaaaa gagtgatcct cgccgacgct aacctcgata 11281 aggtgctttc tgcttacaat aagcacaggg ataagcccat cagggagcag gcagaaaaca 11341 ttatccactt gtttactctg accaacttgg gcgcgcctgc agccttcaag tacttcgaca 11401 ccaccataga cagaaagcgg tacacctcta caaaggaggt cctggacgcc acactgattc 11461 atcagtcaat tacggggctc tatgaaacaa gaatcgacct ctctcagctc ggtggagaca 11521 gcagggctga ccccaagaag aagaggaagg tgtgattttg gacctcgagt cattggacct 11581 cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta 11641 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt 11701 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc 11761 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag 11821 gctttaattt gcgatccccg tctaagaaac cattattatc atgacattaa cctataaaaa 11881 taggcgtatc acgaggccct ttcgtc //